Effect of pro- and metabiotic <em>Lactobacillus delbrueckii D5</em> strain on myocardial resistance to ischemia – reperfusion injury in the rat model of systemic inflammatory response
Aim. To study the effect of lyophilized L. delbrueckii D5, as well as its inactivated culture, during intragastric administration on myocardial resistance to ischemia – reperfusion injury (IRI), markers of inflammation, and intestinal epithelial permeability. Materials and methods. The experiments w...
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Siberian State Medical University (Tomsk)
2024-07-01
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| Series: | Бюллетень сибирской медицины |
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| Online Access: | https://bulletin.ssmu.ru/jour/article/view/5659 |
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| author | Yu. Yu. Borshchev S. M. Minasean N. Yu. Semenova I. Yu. Burovenko O. V. Borshcheva E. Yu. Gritsenko V. A. Sheptitsky A. N. Suvorov M. M. Galagudza |
| author_facet | Yu. Yu. Borshchev S. M. Minasean N. Yu. Semenova I. Yu. Burovenko O. V. Borshcheva E. Yu. Gritsenko V. A. Sheptitsky A. N. Suvorov M. M. Galagudza |
| author_sort | Yu. Yu. Borshchev |
| collection | DOAJ |
| description | Aim. To study the effect of lyophilized L. delbrueckii D5, as well as its inactivated culture, during intragastric administration on myocardial resistance to ischemia – reperfusion injury (IRI), markers of inflammation, and intestinal epithelial permeability. Materials and methods. The experiments were performed on male Wistar rats with a model of systemic inflammatory response syndrome (SIRS). Myocardial IRI was reproduced on an isolated Langendorff heart. Results. A significant increase in the levels of tumor necrosis factor (TNF)α, interleukin (IL)-1β, IL-6, and lactoferrin in SIRS was revealed. The introduction of both inactivated and lyophilized culture of L. delbrueckii D5 resulted in normalization of these changes. Normalization of the increased blood level of lipopolysaccharide in SIRS was also noted with the introduction of both inactivated and lyophilized L. delbrueckii D5. However, the inactivated culture had no effect on the myocardial infarct size, which was increased in the SIRS group compared to the controls, whereas the introduction of the lyophilized strain led to a significant decrease in this parameter. Conclusion. The inactivated culture of Lactobacillus delbrueckii D5 has a pronounced anti-inflammatory effect, but does not impact myocardial resistance to IRI, unlike the lyophilized strain, which requires further research. |
| format | Article |
| id | doaj-art-e8e87cf96dba4f56b7fa34758aab197e |
| institution | DOAJ |
| issn | 1682-0363 1819-3684 |
| language | English |
| publishDate | 2024-07-01 |
| publisher | Siberian State Medical University (Tomsk) |
| record_format | Article |
| series | Бюллетень сибирской медицины |
| spelling | doaj-art-e8e87cf96dba4f56b7fa34758aab197e2025-08-20T03:02:04ZengSiberian State Medical University (Tomsk)Бюллетень сибирской медицины1682-03631819-36842024-07-01232283610.20538/1682-0363-2024-2-28-363069Effect of pro- and metabiotic <em>Lactobacillus delbrueckii D5</em> strain on myocardial resistance to ischemia – reperfusion injury in the rat model of systemic inflammatory responseYu. Yu. Borshchev0S. M. Minasean1N. Yu. Semenova2I. Yu. Burovenko3O. V. Borshcheva4E. Yu. Gritsenko5V. A. Sheptitsky6A. N. Suvorov7M. M. Galagudza8Almazov National Medical Research Center; N.N. Petrov National Medical Research Center (NMRC) of OncologyAlmazov National Medical Research Center; Pavlov First Saint Petersburg State Medical UniversityAlmazov National Medical Research CenterAlmazov National Medical Research CenterAlmazov National Medical Research CenterShevchenko Transnistria State UniversityShevchenko Transnistria State UniversityInstitute of Experimental Medicine; St. Petersburg UniversityAlmazov National Medical Research Center; Pavlov First Saint Petersburg State Medical UniversityAim. To study the effect of lyophilized L. delbrueckii D5, as well as its inactivated culture, during intragastric administration on myocardial resistance to ischemia – reperfusion injury (IRI), markers of inflammation, and intestinal epithelial permeability. Materials and methods. The experiments were performed on male Wistar rats with a model of systemic inflammatory response syndrome (SIRS). Myocardial IRI was reproduced on an isolated Langendorff heart. Results. A significant increase in the levels of tumor necrosis factor (TNF)α, interleukin (IL)-1β, IL-6, and lactoferrin in SIRS was revealed. The introduction of both inactivated and lyophilized culture of L. delbrueckii D5 resulted in normalization of these changes. Normalization of the increased blood level of lipopolysaccharide in SIRS was also noted with the introduction of both inactivated and lyophilized L. delbrueckii D5. However, the inactivated culture had no effect on the myocardial infarct size, which was increased in the SIRS group compared to the controls, whereas the introduction of the lyophilized strain led to a significant decrease in this parameter. Conclusion. The inactivated culture of Lactobacillus delbrueckii D5 has a pronounced anti-inflammatory effect, but does not impact myocardial resistance to IRI, unlike the lyophilized strain, which requires further research.https://bulletin.ssmu.ru/jour/article/view/5659myocardiumischemia – reperfusion injuryinfarct sizelactobacillus delbrueckii d5systemic inflammatory response syndromeleukocytescytokinesprobiotics |
| spellingShingle | Yu. Yu. Borshchev S. M. Minasean N. Yu. Semenova I. Yu. Burovenko O. V. Borshcheva E. Yu. Gritsenko V. A. Sheptitsky A. N. Suvorov M. M. Galagudza Effect of pro- and metabiotic <em>Lactobacillus delbrueckii D5</em> strain on myocardial resistance to ischemia – reperfusion injury in the rat model of systemic inflammatory response Бюллетень сибирской медицины myocardium ischemia – reperfusion injury infarct size lactobacillus delbrueckii d5 systemic inflammatory response syndrome leukocytes cytokines probiotics |
| title | Effect of pro- and metabiotic <em>Lactobacillus delbrueckii D5</em> strain on myocardial resistance to ischemia – reperfusion injury in the rat model of systemic inflammatory response |
| title_full | Effect of pro- and metabiotic <em>Lactobacillus delbrueckii D5</em> strain on myocardial resistance to ischemia – reperfusion injury in the rat model of systemic inflammatory response |
| title_fullStr | Effect of pro- and metabiotic <em>Lactobacillus delbrueckii D5</em> strain on myocardial resistance to ischemia – reperfusion injury in the rat model of systemic inflammatory response |
| title_full_unstemmed | Effect of pro- and metabiotic <em>Lactobacillus delbrueckii D5</em> strain on myocardial resistance to ischemia – reperfusion injury in the rat model of systemic inflammatory response |
| title_short | Effect of pro- and metabiotic <em>Lactobacillus delbrueckii D5</em> strain on myocardial resistance to ischemia – reperfusion injury in the rat model of systemic inflammatory response |
| title_sort | effect of pro and metabiotic em lactobacillus delbrueckii d5 em strain on myocardial resistance to ischemia reperfusion injury in the rat model of systemic inflammatory response |
| topic | myocardium ischemia – reperfusion injury infarct size lactobacillus delbrueckii d5 systemic inflammatory response syndrome leukocytes cytokines probiotics |
| url | https://bulletin.ssmu.ru/jour/article/view/5659 |
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