Ecological and Phytochemical Studies on Euphorbia retusa (Forssk.) from Egyptian Habitat
This study deals with the ecology, phytochemistry, and biological activity investigation of Euphorbia retusa, belonging to Euphorbiaceae family, obtained from Egypt. Ecologically, Euphorbia retusa secretes white sap inhibiting the growth of the other species, so Euphorbia retusa is forming complete...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2018/9143683 |
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| author | Mohamed Shaaban Mahmoud Ali Michel Feussi Tala Abdelaaty Hamed Amal Zaki Hassan |
| author_facet | Mohamed Shaaban Mahmoud Ali Michel Feussi Tala Abdelaaty Hamed Amal Zaki Hassan |
| author_sort | Mohamed Shaaban |
| collection | DOAJ |
| description | This study deals with the ecology, phytochemistry, and biological activity investigation of Euphorbia retusa, belonging to Euphorbiaceae family, obtained from Egypt. Ecologically, Euphorbia retusa secretes white sap inhibiting the growth of the other species, so Euphorbia retusa is forming complete patches. Phytochemical study of the plant was visualized intensively based on its extraction with a protic organic solvent, working up and purifying its entire bioactive compounds using a series of different chromatographic techniques. A broad range of diverse compounds were isolated, namely, 1-hexacosanol (1), 3β-hydroxy-24-methylene-9,19-cyclolanostane; 24-methylenecycloartanol (2), 3β-hydroxy-9,19-cyclolanostane; cyclolaudanol (3), 3β,24S-Ergost-5-en-ol (4), and methyllinoleate. Additionally, GC-MS analysis of the unpolar fractions detected the existence of n-dodecane, methyllaurate, 6,10,14-trimethyl-pentadecan-2-one (5), 6,10-dimethyl-undecan-2-one (6), 2-methyl-hexadecanal (7), methylpalmitate, methyl-9,12,15-octadecatrienoate (8), and n-heneicosane (9). A full assignment for compounds 2 and 3 using 1 and 2 DNMR was carried out herein for the first time. The antimicrobial activity of the strain extract and obtained compounds was studied using a panel of pathogenic bacterial strains. The in vitro cytotoxicity of the compounds as well as the crude extract was studied against the human cervix carcinoma cell line (KB-3-1). |
| format | Article |
| id | doaj-art-e8e660cbf0c3433c9eee40e3c582900d |
| institution | DOAJ |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-e8e660cbf0c3433c9eee40e3c582900d2025-08-20T03:21:09ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732018-01-01201810.1155/2018/91436839143683Ecological and Phytochemical Studies on Euphorbia retusa (Forssk.) from Egyptian HabitatMohamed Shaaban0Mahmoud Ali1Michel Feussi Tala2Abdelaaty Hamed3Amal Zaki Hassan4Chemistry of Natural Compounds Department, Division of Pharmaceutical Industries, National Research Centre, El-Behoos St. 33, Dokki, Cairo 12622, EgyptPlant Ecology and Range Management Department, Desert Research Centre, Cairo, EgyptDepartment of Organic and Biomolecular Chemistry, University of Göttingen, Tammannstrasse 2, 37077 Göttingen, GermanyOrganic and Bioorganic Chemistry, Department of Chemistry, Bielefeld University, Universitätsstraße 25, 33501 Bielefeld, GermanyChemistry of Natural Compounds Department, Division of Pharmaceutical Industries, National Research Centre, El-Behoos St. 33, Dokki, Cairo 12622, EgyptThis study deals with the ecology, phytochemistry, and biological activity investigation of Euphorbia retusa, belonging to Euphorbiaceae family, obtained from Egypt. Ecologically, Euphorbia retusa secretes white sap inhibiting the growth of the other species, so Euphorbia retusa is forming complete patches. Phytochemical study of the plant was visualized intensively based on its extraction with a protic organic solvent, working up and purifying its entire bioactive compounds using a series of different chromatographic techniques. A broad range of diverse compounds were isolated, namely, 1-hexacosanol (1), 3β-hydroxy-24-methylene-9,19-cyclolanostane; 24-methylenecycloartanol (2), 3β-hydroxy-9,19-cyclolanostane; cyclolaudanol (3), 3β,24S-Ergost-5-en-ol (4), and methyllinoleate. Additionally, GC-MS analysis of the unpolar fractions detected the existence of n-dodecane, methyllaurate, 6,10,14-trimethyl-pentadecan-2-one (5), 6,10-dimethyl-undecan-2-one (6), 2-methyl-hexadecanal (7), methylpalmitate, methyl-9,12,15-octadecatrienoate (8), and n-heneicosane (9). A full assignment for compounds 2 and 3 using 1 and 2 DNMR was carried out herein for the first time. The antimicrobial activity of the strain extract and obtained compounds was studied using a panel of pathogenic bacterial strains. The in vitro cytotoxicity of the compounds as well as the crude extract was studied against the human cervix carcinoma cell line (KB-3-1).http://dx.doi.org/10.1155/2018/9143683 |
| spellingShingle | Mohamed Shaaban Mahmoud Ali Michel Feussi Tala Abdelaaty Hamed Amal Zaki Hassan Ecological and Phytochemical Studies on Euphorbia retusa (Forssk.) from Egyptian Habitat Journal of Analytical Methods in Chemistry |
| title | Ecological and Phytochemical Studies on Euphorbia retusa (Forssk.) from Egyptian Habitat |
| title_full | Ecological and Phytochemical Studies on Euphorbia retusa (Forssk.) from Egyptian Habitat |
| title_fullStr | Ecological and Phytochemical Studies on Euphorbia retusa (Forssk.) from Egyptian Habitat |
| title_full_unstemmed | Ecological and Phytochemical Studies on Euphorbia retusa (Forssk.) from Egyptian Habitat |
| title_short | Ecological and Phytochemical Studies on Euphorbia retusa (Forssk.) from Egyptian Habitat |
| title_sort | ecological and phytochemical studies on euphorbia retusa forssk from egyptian habitat |
| url | http://dx.doi.org/10.1155/2018/9143683 |
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