Antimicrobial and anti-quorum sensing activities of giant fennel (ferula elaeochytris korovin) from the hatay region
<b>Aim: </b>Today, increasing antibiotic resistance has focused the science world on the discovery of new antimicrobial molecules or the development of alternative methods of struggle. One of the alternative methods is thought to be inhibition of bacterial quorum sensing (QS). Because th...
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| Format: | Article |
| Language: | English |
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Selcuk University Press
2020-10-01
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| Series: | Eurasian Journal of Veterinary Sciences |
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| Online Access: | http://eurasianjvetsci.org/pdf.php3?id=1300 |
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| author | Enis Fuat Tüfekci Anfal Alkateeb Sarah Akar Orhan Çorum Yasemin Çelik Altunoğlu Mehmet Cengiz Baloğlu Muammer Kiraz Nilay Çöplü |
| author_facet | Enis Fuat Tüfekci Anfal Alkateeb Sarah Akar Orhan Çorum Yasemin Çelik Altunoğlu Mehmet Cengiz Baloğlu Muammer Kiraz Nilay Çöplü |
| author_sort | Enis Fuat Tüfekci |
| collection | DOAJ |
| description | <b>Aim: </b>Today, increasing antibiotic resistance has focused the science world on
the discovery of new antimicrobial molecules or the development of alternative
methods of struggle. One of the alternative methods is thought to be inhibition
of bacterial quorum sensing (QS). Because the QS system performs a
crucial part in the synthesis of virulence factors in bacteria. Numerous medicinal
plants are known to have promising antimicrobial and anti-QS activities.
One of these plants may be giant fennel (Ferula elaeochytris Korovin), which
has been extensively used in Anatolia for years. Therefore, it was aimed to investigate
the antimicrobial and anti-QS activities of the root extract of F. elaeochytris
Korovin in this study. <p>
<b>Materials and Methods:</b> The antimicrobial activity of the extract was screened
by disc diffusion assay against various microorganisms. Minimum inhibitory
concentration (MIC) and minimum bactericidal concentration (MBC) of
the extract against sensitive strain(s) were determined using broth microdilution
assay. Anti-QS activity investigated on inhibition of violacein and pyocyanin
production in Chromobacterium violaceum and Pseudomonas aeruginosa
bioreporter strains, respectively.<p>
<b>Results:</b> The extract exhibited the antimicrobial activity against only Staphylococcus
aureus (zone of inhibition (ZOI) = 9.3±0.6 mm). The MIC and the
MBC values were determined as 4.4±1.9 mg/mL and >105 mg/mL, respectively.
No inhibition of violacein production was detected. Pyocyanin production
was reduced by 60% and 82% at sub-MIC concentrations (at 1.64 mg/mL and
3.28 mg/mL, respectively) as against the control (p<0.05) without suppressing
bacterial growth.<p>
<b>Conclusion:</b> This study shows that the root extract of F. elaeochytris Korovin
may be a good candidate to develop antistaphylococcal and anti-QS agents. |
| format | Article |
| id | doaj-art-e8de423f444b40f88553a15cd5800779 |
| institution | DOAJ |
| issn | 1309-6958 2146-1953 |
| language | English |
| publishDate | 2020-10-01 |
| publisher | Selcuk University Press |
| record_format | Article |
| series | Eurasian Journal of Veterinary Sciences |
| spelling | doaj-art-e8de423f444b40f88553a15cd58007792025-08-20T02:52:06ZengSelcuk University PressEurasian Journal of Veterinary Sciences1309-69582146-19532020-10-013632142201300Antimicrobial and anti-quorum sensing activities of giant fennel (ferula elaeochytris korovin) from the hatay regionEnis Fuat Tüfekci0Anfal Alkateeb1Sarah Akar2Orhan Çorum3Yasemin Çelik Altunoğlu4Mehmet Cengiz Baloğlu5Muammer Kiraz6Nilay Çöplü7Kastamonu University, Medicine Faculty, Department of Medical Microbiology, Kastamonu, TurkeyKastamonu University, Engineering and Architecture Faculty, Department of Genetics and Bioengineering, Kastamonu, TurkeyKastamonu University, Engineering and Architecture Faculty, Department of Genetics and Bioengineering, Kastamonu, TurkeyKastamonu University, Veterinary Faculty, Department of Pharmacology and Toxicology, Kastamonu, TurkeyKastamonu University, Engineering and Architecture Faculty, Department of Genetics and Bioengineering, Kastamonu, TurkeyKastamonu University, Engineering and Architecture Faculty, Department of Genetics and Bioengineering, Kastamonu, TurkeyKastamonu University, Medicine Faculty, Department of Medical Microbiology, Kastamonu, TurkeyKastamonu University, Medicine Faculty, Department of Medical Microbiology, Kastamonu, Turkey<b>Aim: </b>Today, increasing antibiotic resistance has focused the science world on the discovery of new antimicrobial molecules or the development of alternative methods of struggle. One of the alternative methods is thought to be inhibition of bacterial quorum sensing (QS). Because the QS system performs a crucial part in the synthesis of virulence factors in bacteria. Numerous medicinal plants are known to have promising antimicrobial and anti-QS activities. One of these plants may be giant fennel (Ferula elaeochytris Korovin), which has been extensively used in Anatolia for years. Therefore, it was aimed to investigate the antimicrobial and anti-QS activities of the root extract of F. elaeochytris Korovin in this study. <p> <b>Materials and Methods:</b> The antimicrobial activity of the extract was screened by disc diffusion assay against various microorganisms. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the extract against sensitive strain(s) were determined using broth microdilution assay. Anti-QS activity investigated on inhibition of violacein and pyocyanin production in Chromobacterium violaceum and Pseudomonas aeruginosa bioreporter strains, respectively.<p> <b>Results:</b> The extract exhibited the antimicrobial activity against only Staphylococcus aureus (zone of inhibition (ZOI) = 9.3±0.6 mm). The MIC and the MBC values were determined as 4.4±1.9 mg/mL and >105 mg/mL, respectively. No inhibition of violacein production was detected. Pyocyanin production was reduced by 60% and 82% at sub-MIC concentrations (at 1.64 mg/mL and 3.28 mg/mL, respectively) as against the control (p<0.05) without suppressing bacterial growth.<p> <b>Conclusion:</b> This study shows that the root extract of F. elaeochytris Korovin may be a good candidate to develop antistaphylococcal and anti-QS agents.http://eurasianjvetsci.org/pdf.php3?id=1300antimicrobialextractferula elaeochytris korovinpyocyaninquorum sensing |
| spellingShingle | Enis Fuat Tüfekci Anfal Alkateeb Sarah Akar Orhan Çorum Yasemin Çelik Altunoğlu Mehmet Cengiz Baloğlu Muammer Kiraz Nilay Çöplü Antimicrobial and anti-quorum sensing activities of giant fennel (ferula elaeochytris korovin) from the hatay region Eurasian Journal of Veterinary Sciences antimicrobial extract ferula elaeochytris korovin pyocyanin quorum sensing |
| title | Antimicrobial and anti-quorum sensing activities of giant fennel (ferula elaeochytris korovin) from the hatay region |
| title_full | Antimicrobial and anti-quorum sensing activities of giant fennel (ferula elaeochytris korovin) from the hatay region |
| title_fullStr | Antimicrobial and anti-quorum sensing activities of giant fennel (ferula elaeochytris korovin) from the hatay region |
| title_full_unstemmed | Antimicrobial and anti-quorum sensing activities of giant fennel (ferula elaeochytris korovin) from the hatay region |
| title_short | Antimicrobial and anti-quorum sensing activities of giant fennel (ferula elaeochytris korovin) from the hatay region |
| title_sort | antimicrobial and anti quorum sensing activities of giant fennel ferula elaeochytris korovin from the hatay region |
| topic | antimicrobial extract ferula elaeochytris korovin pyocyanin quorum sensing |
| url | http://eurasianjvetsci.org/pdf.php3?id=1300 |
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