Robust genome editing activity and the applications of enhanced miniature CRISPR-Cas12f1
Abstract With recent advancements in gene editing technology using the CRISPR/Cas system, there is a demand for more effective gene editors. A key factor facilitating efficient gene editing is effective CRISPR delivery into cells, which is known to be associated with the size of the CRISPR system. A...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-01-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-025-56048-w |
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| author | Soo-Ji Park Sungjin Ju Won Jun Jung Tae Yeong Jeong Da Eun Yoon Jang Hyeon Lee Jiyun Yang Hojin Lee Jungmin Choi Hyeon Soo Kim Kyoungmi Kim |
| author_facet | Soo-Ji Park Sungjin Ju Won Jun Jung Tae Yeong Jeong Da Eun Yoon Jang Hyeon Lee Jiyun Yang Hojin Lee Jungmin Choi Hyeon Soo Kim Kyoungmi Kim |
| author_sort | Soo-Ji Park |
| collection | DOAJ |
| description | Abstract With recent advancements in gene editing technology using the CRISPR/Cas system, there is a demand for more effective gene editors. A key factor facilitating efficient gene editing is effective CRISPR delivery into cells, which is known to be associated with the size of the CRISPR system. Accordingly, compact CRISPR-Cas systems derived from various strains are discovered, among which Un1Cas12f1 is 2.6 times smaller than SpCas9, providing advantages for gene therapy research. Despite extensive engineering efforts to improve Un1Cas12f1, the editing efficiency of Un1Cas12f1 is still shown to be low depending on the target site. To overcome this limitation, we develop enhanced Cas12f1 (eCas12f1), which exhibits gene editing activity similar to SpCas9 and AsCpf1, even in gene targets where previously improved Un1Cas12f1 variants showed low gene editing efficiency. Furthermore, we demonstrate that eCas12f1 efficiently induces apoptosis in cancer cells and is compatible with base editing and regulation of gene expression, verifying its high utility and applicability in gene therapy research. |
| format | Article |
| id | doaj-art-e8dc0c404a8b4842975241f58eaca0df |
| institution | Kabale University |
| issn | 2041-1723 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-e8dc0c404a8b4842975241f58eaca0df2025-01-19T12:31:30ZengNature PortfolioNature Communications2041-17232025-01-0116111210.1038/s41467-025-56048-wRobust genome editing activity and the applications of enhanced miniature CRISPR-Cas12f1Soo-Ji Park0Sungjin Ju1Won Jun Jung2Tae Yeong Jeong3Da Eun Yoon4Jang Hyeon Lee5Jiyun Yang6Hojin Lee7Jungmin Choi8Hyeon Soo Kim9Kyoungmi Kim10Department of Physiology, Korea University College of MedicineDepartment of Physiology, Korea University College of MedicineDepartment of Physiology, Korea University College of MedicineDepartment of Physiology, Korea University College of MedicineDepartment of Physiology, Korea University College of MedicineDepartment of Physiology, Korea University College of MedicineDepartment of Physiology, Korea University College of MedicineDepartment of Biomedical Sciences, Korea University College of MedicineDepartment of Biomedical Sciences, Korea University College of MedicineDepartment of Biomedical Sciences, Korea University College of MedicineDepartment of Physiology, Korea University College of MedicineAbstract With recent advancements in gene editing technology using the CRISPR/Cas system, there is a demand for more effective gene editors. A key factor facilitating efficient gene editing is effective CRISPR delivery into cells, which is known to be associated with the size of the CRISPR system. Accordingly, compact CRISPR-Cas systems derived from various strains are discovered, among which Un1Cas12f1 is 2.6 times smaller than SpCas9, providing advantages for gene therapy research. Despite extensive engineering efforts to improve Un1Cas12f1, the editing efficiency of Un1Cas12f1 is still shown to be low depending on the target site. To overcome this limitation, we develop enhanced Cas12f1 (eCas12f1), which exhibits gene editing activity similar to SpCas9 and AsCpf1, even in gene targets where previously improved Un1Cas12f1 variants showed low gene editing efficiency. Furthermore, we demonstrate that eCas12f1 efficiently induces apoptosis in cancer cells and is compatible with base editing and regulation of gene expression, verifying its high utility and applicability in gene therapy research.https://doi.org/10.1038/s41467-025-56048-w |
| spellingShingle | Soo-Ji Park Sungjin Ju Won Jun Jung Tae Yeong Jeong Da Eun Yoon Jang Hyeon Lee Jiyun Yang Hojin Lee Jungmin Choi Hyeon Soo Kim Kyoungmi Kim Robust genome editing activity and the applications of enhanced miniature CRISPR-Cas12f1 Nature Communications |
| title | Robust genome editing activity and the applications of enhanced miniature CRISPR-Cas12f1 |
| title_full | Robust genome editing activity and the applications of enhanced miniature CRISPR-Cas12f1 |
| title_fullStr | Robust genome editing activity and the applications of enhanced miniature CRISPR-Cas12f1 |
| title_full_unstemmed | Robust genome editing activity and the applications of enhanced miniature CRISPR-Cas12f1 |
| title_short | Robust genome editing activity and the applications of enhanced miniature CRISPR-Cas12f1 |
| title_sort | robust genome editing activity and the applications of enhanced miniature crispr cas12f1 |
| url | https://doi.org/10.1038/s41467-025-56048-w |
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