Phenotypic and Genotypic Antibiotic Resistance of Bacteria Isolated from Ready-to-eat Salted Seafood

Safe food production faces significant challenges from both disease-causing bacteria and antibiotic resistant bacteria, as both pose serious risks to public health and food safety. This study investigated the presence of antibiotic-resistant bacteria in salted seafood (lakerda) samples obtained from...

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Main Authors: Dilek Kahraman Yılmaz, Nermin Berik
Format: Article
Language:English
Published: Istanbul University Press 2025-01-01
Series:Aquatic Sciences and Engineering
Subjects:
Online Access:https://cdn.istanbul.edu.tr/file/JTA6CLJ8T5/5835AFD777D748DBAFDA2BE30BEC52CB
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author Dilek Kahraman Yılmaz
Nermin Berik
author_facet Dilek Kahraman Yılmaz
Nermin Berik
author_sort Dilek Kahraman Yılmaz
collection DOAJ
description Safe food production faces significant challenges from both disease-causing bacteria and antibiotic resistant bacteria, as both pose serious risks to public health and food safety. This study investigated the presence of antibiotic-resistant bacteria in salted seafood (lakerda) samples obtained from fishermen and fish markets. Phenotypic analysis revealed that lakerda samples collected from fishermen contained bacteria with multi-antibiotic resistance, including Pseudomonas fluorescens, Staphylococcus haemolyticus, and Staphylococcus equorum. Carnobacterium maltaromaticum, Carnobacterium mobile, and Vibrio hibernica species were isolated in larkerda samples sold by fish markets. It was determined that among isolated bacteria, V. rumoiensis did not contain any of the genotypically tested genes. However, P. fluorescens carried blaTEM, qnrB, qnrS, blaZ, and msrA; S. haemolyticus harbored blaTEM, tetK, dfrD, blaZ, msrA, msrB, and mecA; C. maltaromaticum possessed blaTEM, qnrA, qnrB, qnrS, strA-strB, aphAI-IAB, and mecA; C. mobile included blaTEM, blaZ, msrA, dfrD, and mecA; and V. hibernica carried blaTEM, blaZ, mecA, and VanA. In addition, S. pasteuri and S. equorum had the mecA resistance gene. In conclusion, public health needs to provide hygiene conditions in the preparation of lakerda, determine the ways of transmission, take precautions, and raise awareness of producers and consumers.
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spelling doaj-art-e8dada16a3b141e38704a4134a688de92025-08-20T03:52:52ZengIstanbul University PressAquatic Sciences and Engineering2602-473X2025-01-0140191710.26650/ASE20241589821123456Phenotypic and Genotypic Antibiotic Resistance of Bacteria Isolated from Ready-to-eat Salted SeafoodDilek Kahraman Yılmaz0https://orcid.org/0000-0002-9626-5446Nermin Berik1https://orcid.org/0000-0003-3015-8688Çanakkale Onsekiz Mart Üniversitesi, Canakkale, TurkiyeÇanakkale Onsekiz Mart Üniversitesi, Canakkale, TurkiyeSafe food production faces significant challenges from both disease-causing bacteria and antibiotic resistant bacteria, as both pose serious risks to public health and food safety. This study investigated the presence of antibiotic-resistant bacteria in salted seafood (lakerda) samples obtained from fishermen and fish markets. Phenotypic analysis revealed that lakerda samples collected from fishermen contained bacteria with multi-antibiotic resistance, including Pseudomonas fluorescens, Staphylococcus haemolyticus, and Staphylococcus equorum. Carnobacterium maltaromaticum, Carnobacterium mobile, and Vibrio hibernica species were isolated in larkerda samples sold by fish markets. It was determined that among isolated bacteria, V. rumoiensis did not contain any of the genotypically tested genes. However, P. fluorescens carried blaTEM, qnrB, qnrS, blaZ, and msrA; S. haemolyticus harbored blaTEM, tetK, dfrD, blaZ, msrA, msrB, and mecA; C. maltaromaticum possessed blaTEM, qnrA, qnrB, qnrS, strA-strB, aphAI-IAB, and mecA; C. mobile included blaTEM, blaZ, msrA, dfrD, and mecA; and V. hibernica carried blaTEM, blaZ, mecA, and VanA. In addition, S. pasteuri and S. equorum had the mecA resistance gene. In conclusion, public health needs to provide hygiene conditions in the preparation of lakerda, determine the ways of transmission, take precautions, and raise awareness of producers and consumers.https://cdn.istanbul.edu.tr/file/JTA6CLJ8T5/5835AFD777D748DBAFDA2BE30BEC52CBfood safetylakerdaantibiotic resistanceresistance genesmicrobiology
spellingShingle Dilek Kahraman Yılmaz
Nermin Berik
Phenotypic and Genotypic Antibiotic Resistance of Bacteria Isolated from Ready-to-eat Salted Seafood
Aquatic Sciences and Engineering
food safety
lakerda
antibiotic resistance
resistance genes
microbiology
title Phenotypic and Genotypic Antibiotic Resistance of Bacteria Isolated from Ready-to-eat Salted Seafood
title_full Phenotypic and Genotypic Antibiotic Resistance of Bacteria Isolated from Ready-to-eat Salted Seafood
title_fullStr Phenotypic and Genotypic Antibiotic Resistance of Bacteria Isolated from Ready-to-eat Salted Seafood
title_full_unstemmed Phenotypic and Genotypic Antibiotic Resistance of Bacteria Isolated from Ready-to-eat Salted Seafood
title_short Phenotypic and Genotypic Antibiotic Resistance of Bacteria Isolated from Ready-to-eat Salted Seafood
title_sort phenotypic and genotypic antibiotic resistance of bacteria isolated from ready to eat salted seafood
topic food safety
lakerda
antibiotic resistance
resistance genes
microbiology
url https://cdn.istanbul.edu.tr/file/JTA6CLJ8T5/5835AFD777D748DBAFDA2BE30BEC52CB
work_keys_str_mv AT dilekkahramanyılmaz phenotypicandgenotypicantibioticresistanceofbacteriaisolatedfromreadytoeatsaltedseafood
AT nerminberik phenotypicandgenotypicantibioticresistanceofbacteriaisolatedfromreadytoeatsaltedseafood