Gyne production is regulated by the brood in a social bee (Bombus impatiens)

Sexual production in social insects marks the peak of colony development, yet the mechanisms regulating it remain unclear. We investigated the role of brood in colony development, worker reproduction and sexual production in Bombus impatiens. While larvae are known to reduce worker egg laying and en...

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Main Authors: Etya Amsalem, Priscila K. F. Santos, Ella Messner, Cameron Murray
Format: Article
Language:English
Published: The Royal Society 2025-08-01
Series:Royal Society Open Science
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Online Access:https://royalsocietypublishing.org/doi/10.1098/rsos.241906
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author Etya Amsalem
Priscila K. F. Santos
Ella Messner
Cameron Murray
author_facet Etya Amsalem
Priscila K. F. Santos
Ella Messner
Cameron Murray
author_sort Etya Amsalem
collection DOAJ
description Sexual production in social insects marks the peak of colony development, yet the mechanisms regulating it remain unclear. We investigated the role of brood in colony development, worker reproduction and sexual production in Bombus impatiens. While larvae are known to reduce worker egg laying and enhance the queen’s reproductive inhibition, these effects were previously tested only in small groups. We manipulated brood size in full-sized, young colonies by doubling or removing brood and monitored development. Colonies with doubled brood produced significantly more gynes, independent of the number of workers, while reduced-brood colonies exhibited a non-significant increase in male production that was driven by colony size. Worker ovary activation was lower in double-brood colonies, with no change in aggression. A follow-up experiment directly testing the effect of colony size showed that higher worker density led to higher ovary activation in workers but did not affect sexual production. These results suggest that brood strongly influences colony development and sexual production, possibly reflecting an extended phenotype of the queen, whereas worker ovary activation appears to be a more flexible process influenced by either brood presence or colony size. Understanding brood dynamics may be key to understanding the evolution of female castes in social insects.
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spelling doaj-art-e8d48fec4a524b90aca52ef3830854122025-08-20T04:02:12ZengThe Royal SocietyRoyal Society Open Science2054-57032025-08-0112810.1098/rsos.241906Gyne production is regulated by the brood in a social bee (Bombus impatiens)Etya Amsalem0Priscila K. F. Santos1Ella Messner2Cameron Murray3Department of Entomology, Penn State University, University Park, PA, USADepartment of Entomology, Penn State University, University Park, PA, USADepartment of Entomology, Penn State University, University Park, PA, USADepartment of Entomology, Penn State University, University Park, PA, USASexual production in social insects marks the peak of colony development, yet the mechanisms regulating it remain unclear. We investigated the role of brood in colony development, worker reproduction and sexual production in Bombus impatiens. While larvae are known to reduce worker egg laying and enhance the queen’s reproductive inhibition, these effects were previously tested only in small groups. We manipulated brood size in full-sized, young colonies by doubling or removing brood and monitored development. Colonies with doubled brood produced significantly more gynes, independent of the number of workers, while reduced-brood colonies exhibited a non-significant increase in male production that was driven by colony size. Worker ovary activation was lower in double-brood colonies, with no change in aggression. A follow-up experiment directly testing the effect of colony size showed that higher worker density led to higher ovary activation in workers but did not affect sexual production. These results suggest that brood strongly influences colony development and sexual production, possibly reflecting an extended phenotype of the queen, whereas worker ovary activation appears to be a more flexible process influenced by either brood presence or colony size. Understanding brood dynamics may be key to understanding the evolution of female castes in social insects.https://royalsocietypublishing.org/doi/10.1098/rsos.241906broodsocialitycaste determinationreproductiongynesdensity
spellingShingle Etya Amsalem
Priscila K. F. Santos
Ella Messner
Cameron Murray
Gyne production is regulated by the brood in a social bee (Bombus impatiens)
Royal Society Open Science
brood
sociality
caste determination
reproduction
gynes
density
title Gyne production is regulated by the brood in a social bee (Bombus impatiens)
title_full Gyne production is regulated by the brood in a social bee (Bombus impatiens)
title_fullStr Gyne production is regulated by the brood in a social bee (Bombus impatiens)
title_full_unstemmed Gyne production is regulated by the brood in a social bee (Bombus impatiens)
title_short Gyne production is regulated by the brood in a social bee (Bombus impatiens)
title_sort gyne production is regulated by the brood in a social bee bombus impatiens
topic brood
sociality
caste determination
reproduction
gynes
density
url https://royalsocietypublishing.org/doi/10.1098/rsos.241906
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AT priscilakfsantos gyneproductionisregulatedbythebroodinasocialbeebombusimpatiens
AT ellamessner gyneproductionisregulatedbythebroodinasocialbeebombusimpatiens
AT cameronmurray gyneproductionisregulatedbythebroodinasocialbeebombusimpatiens