Geographical mapping and seroprevalence of Burkholderia pseudomallei amongst livestock species in Lao People's Democratic Republic.

The Gram-negative bacterium Burkholderia pseudomallei causes a severe infectious disease known as melioidosis in humans and animals. It is considered endemic in tropical countries, including Thailand, Lao PDR (Laos), and Northern Australia. B. pseudomallei is a saprophyte found in contaminated soil...

Full description

Saved in:
Bibliographic Details
Main Authors: Suwei Zheng, James R Young, Syseng Khounsy, Phouvong Phommachanh, Peter Christensen, Watthana Theppangna, Tom Hughes, Adisone Temmerath, Alex Inthavong, Phoummavanh Inthapanya, Sivone Panyasith, Koukeo Phommasone, Direk Limmathurotsakul, Elizabeth A Ashley, Stuart D Blacksell, Michael P Ward
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2025-02-01
Series:PLoS Neglected Tropical Diseases
Online Access:https://doi.org/10.1371/journal.pntd.0012711
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1850040105041920000
author Suwei Zheng
James R Young
Syseng Khounsy
Phouvong Phommachanh
Peter Christensen
Watthana Theppangna
Tom Hughes
Adisone Temmerath
Alex Inthavong
Phoummavanh Inthapanya
Sivone Panyasith
Koukeo Phommasone
Direk Limmathurotsakul
Elizabeth A Ashley
Stuart D Blacksell
Michael P Ward
author_facet Suwei Zheng
James R Young
Syseng Khounsy
Phouvong Phommachanh
Peter Christensen
Watthana Theppangna
Tom Hughes
Adisone Temmerath
Alex Inthavong
Phoummavanh Inthapanya
Sivone Panyasith
Koukeo Phommasone
Direk Limmathurotsakul
Elizabeth A Ashley
Stuart D Blacksell
Michael P Ward
author_sort Suwei Zheng
collection DOAJ
description The Gram-negative bacterium Burkholderia pseudomallei causes a severe infectious disease known as melioidosis in humans and animals. It is considered endemic in tropical countries, including Thailand, Lao PDR (Laos), and Northern Australia. B. pseudomallei is a saprophyte found in contaminated soil and surface water. Humans and animals can become infected via direct exposure to contaminated water or soil and inhalation of dust or water droplets. Despite the high morbidity and mortality rates of melioidosis, there is a lack of knowledge of its geographical distribution and seroprevalence, even within endemic countries, raising a significant public health concern. For a better understanding of melioidosis in livestock in Laos, both as an animal health concern and as an indicator of human risk, we collected serum samples from an abattoir monitoring program for B. pseudomallei antibody testing using the Indirect Haemagglutination Assay (IHA). Out of the 917 sera collected, major findings included the identification of a significant cluster (p = 0.041) in the southwest border region adjoining northeastern Thailand, in the province of Savannakhet in Laos. Sera collected in January 2020 had the highest B. pseudomallei seroprevalence (17.0%), and cattle had the highest seroprevalence (22.8%), followed by buffalo (19.7%) and swine (4.0%). The B. pseudomallei seroprevalence results among the common livestock species and the maps generated can assist with future monitoring, prevention, and detection of melioidosis in Laos.
format Article
id doaj-art-e8cfaad6e9b942819f35a421244a2773
institution DOAJ
issn 1935-2727
1935-2735
language English
publishDate 2025-02-01
publisher Public Library of Science (PLoS)
record_format Article
series PLoS Neglected Tropical Diseases
spelling doaj-art-e8cfaad6e9b942819f35a421244a27732025-08-20T02:56:09ZengPublic Library of Science (PLoS)PLoS Neglected Tropical Diseases1935-27271935-27352025-02-01192e001271110.1371/journal.pntd.0012711Geographical mapping and seroprevalence of Burkholderia pseudomallei amongst livestock species in Lao People's Democratic Republic.Suwei ZhengJames R YoungSyseng KhounsyPhouvong PhommachanhPeter ChristensenWatthana TheppangnaTom HughesAdisone TemmerathAlex InthavongPhoummavanh InthapanyaSivone PanyasithKoukeo PhommasoneDirek LimmathurotsakulElizabeth A AshleyStuart D BlacksellMichael P WardThe Gram-negative bacterium Burkholderia pseudomallei causes a severe infectious disease known as melioidosis in humans and animals. It is considered endemic in tropical countries, including Thailand, Lao PDR (Laos), and Northern Australia. B. pseudomallei is a saprophyte found in contaminated soil and surface water. Humans and animals can become infected via direct exposure to contaminated water or soil and inhalation of dust or water droplets. Despite the high morbidity and mortality rates of melioidosis, there is a lack of knowledge of its geographical distribution and seroprevalence, even within endemic countries, raising a significant public health concern. For a better understanding of melioidosis in livestock in Laos, both as an animal health concern and as an indicator of human risk, we collected serum samples from an abattoir monitoring program for B. pseudomallei antibody testing using the Indirect Haemagglutination Assay (IHA). Out of the 917 sera collected, major findings included the identification of a significant cluster (p = 0.041) in the southwest border region adjoining northeastern Thailand, in the province of Savannakhet in Laos. Sera collected in January 2020 had the highest B. pseudomallei seroprevalence (17.0%), and cattle had the highest seroprevalence (22.8%), followed by buffalo (19.7%) and swine (4.0%). The B. pseudomallei seroprevalence results among the common livestock species and the maps generated can assist with future monitoring, prevention, and detection of melioidosis in Laos.https://doi.org/10.1371/journal.pntd.0012711
spellingShingle Suwei Zheng
James R Young
Syseng Khounsy
Phouvong Phommachanh
Peter Christensen
Watthana Theppangna
Tom Hughes
Adisone Temmerath
Alex Inthavong
Phoummavanh Inthapanya
Sivone Panyasith
Koukeo Phommasone
Direk Limmathurotsakul
Elizabeth A Ashley
Stuart D Blacksell
Michael P Ward
Geographical mapping and seroprevalence of Burkholderia pseudomallei amongst livestock species in Lao People's Democratic Republic.
PLoS Neglected Tropical Diseases
title Geographical mapping and seroprevalence of Burkholderia pseudomallei amongst livestock species in Lao People's Democratic Republic.
title_full Geographical mapping and seroprevalence of Burkholderia pseudomallei amongst livestock species in Lao People's Democratic Republic.
title_fullStr Geographical mapping and seroprevalence of Burkholderia pseudomallei amongst livestock species in Lao People's Democratic Republic.
title_full_unstemmed Geographical mapping and seroprevalence of Burkholderia pseudomallei amongst livestock species in Lao People's Democratic Republic.
title_short Geographical mapping and seroprevalence of Burkholderia pseudomallei amongst livestock species in Lao People's Democratic Republic.
title_sort geographical mapping and seroprevalence of burkholderia pseudomallei amongst livestock species in lao people s democratic republic
url https://doi.org/10.1371/journal.pntd.0012711
work_keys_str_mv AT suweizheng geographicalmappingandseroprevalenceofburkholderiapseudomalleiamongstlivestockspeciesinlaopeoplesdemocraticrepublic
AT jamesryoung geographicalmappingandseroprevalenceofburkholderiapseudomalleiamongstlivestockspeciesinlaopeoplesdemocraticrepublic
AT sysengkhounsy geographicalmappingandseroprevalenceofburkholderiapseudomalleiamongstlivestockspeciesinlaopeoplesdemocraticrepublic
AT phouvongphommachanh geographicalmappingandseroprevalenceofburkholderiapseudomalleiamongstlivestockspeciesinlaopeoplesdemocraticrepublic
AT peterchristensen geographicalmappingandseroprevalenceofburkholderiapseudomalleiamongstlivestockspeciesinlaopeoplesdemocraticrepublic
AT watthanatheppangna geographicalmappingandseroprevalenceofburkholderiapseudomalleiamongstlivestockspeciesinlaopeoplesdemocraticrepublic
AT tomhughes geographicalmappingandseroprevalenceofburkholderiapseudomalleiamongstlivestockspeciesinlaopeoplesdemocraticrepublic
AT adisonetemmerath geographicalmappingandseroprevalenceofburkholderiapseudomalleiamongstlivestockspeciesinlaopeoplesdemocraticrepublic
AT alexinthavong geographicalmappingandseroprevalenceofburkholderiapseudomalleiamongstlivestockspeciesinlaopeoplesdemocraticrepublic
AT phoummavanhinthapanya geographicalmappingandseroprevalenceofburkholderiapseudomalleiamongstlivestockspeciesinlaopeoplesdemocraticrepublic
AT sivonepanyasith geographicalmappingandseroprevalenceofburkholderiapseudomalleiamongstlivestockspeciesinlaopeoplesdemocraticrepublic
AT koukeophommasone geographicalmappingandseroprevalenceofburkholderiapseudomalleiamongstlivestockspeciesinlaopeoplesdemocraticrepublic
AT direklimmathurotsakul geographicalmappingandseroprevalenceofburkholderiapseudomalleiamongstlivestockspeciesinlaopeoplesdemocraticrepublic
AT elizabethaashley geographicalmappingandseroprevalenceofburkholderiapseudomalleiamongstlivestockspeciesinlaopeoplesdemocraticrepublic
AT stuartdblacksell geographicalmappingandseroprevalenceofburkholderiapseudomalleiamongstlivestockspeciesinlaopeoplesdemocraticrepublic
AT michaelpward geographicalmappingandseroprevalenceofburkholderiapseudomalleiamongstlivestockspeciesinlaopeoplesdemocraticrepublic