Assessing banana stalk susceptibility to pathogens and their virulence
The purpose of this protocol is to assess (a) the virulence of fungi on banana stalks and (b) the susceptibility of a banana stalk cutting modality/cultivar to a pathogen. The principle, plant material used, duration and expected results are presented. The materials and the five procedural steps—sta...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-06-01
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| Series: | MethodsX |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2215016125000901 |
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| author | T. Desmarez P. Brat L. Lassois B. Barral O. Hubert |
| author_facet | T. Desmarez P. Brat L. Lassois B. Barral O. Hubert |
| author_sort | T. Desmarez |
| collection | DOAJ |
| description | The purpose of this protocol is to assess (a) the virulence of fungi on banana stalks and (b) the susceptibility of a banana stalk cutting modality/cultivar to a pathogen. The principle, plant material used, duration and expected results are presented. The materials and the five procedural steps—stalk sampling, inoculum and plant material preparation, pathogen inoculation, incubation, and evaluation of stalk necrosis—are detailed. Inoculum virulence and banana stalk susceptibility to pathogenic fungi are determined by measuring the proportion of necrosis. |
| format | Article |
| id | doaj-art-e8cc4b0066634c42a2445f77ea036f49 |
| institution | Kabale University |
| issn | 2215-0161 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | MethodsX |
| spelling | doaj-art-e8cc4b0066634c42a2445f77ea036f492025-08-20T03:24:07ZengElsevierMethodsX2215-01612025-06-011410324410.1016/j.mex.2025.103244Assessing banana stalk susceptibility to pathogens and their virulenceT. Desmarez0P. Brat1L. Lassois2B. Barral3O. Hubert4Liege University, Gembloux Agro Bio-Tech, Gembloux 5030, Belgium; Corresponding author.CIRAD, Neufchâteau station, Capesterre-Belle-Eau, Sainte-Marie 97130, GuadeloupeLiege University, Gembloux Agro Bio-Tech, Gembloux 5030, BelgiumCIRAD, Neufchâteau station, Capesterre-Belle-Eau, Sainte-Marie 97130, GuadeloupeCIRAD, Neufchâteau station, Capesterre-Belle-Eau, Sainte-Marie 97130, GuadeloupeThe purpose of this protocol is to assess (a) the virulence of fungi on banana stalks and (b) the susceptibility of a banana stalk cutting modality/cultivar to a pathogen. The principle, plant material used, duration and expected results are presented. The materials and the five procedural steps—stalk sampling, inoculum and plant material preparation, pathogen inoculation, incubation, and evaluation of stalk necrosis—are detailed. Inoculum virulence and banana stalk susceptibility to pathogenic fungi are determined by measuring the proportion of necrosis.http://www.sciencedirect.com/science/article/pii/S2215016125000901Assessing banana stalk susceptibility to pathogens and their virulence |
| spellingShingle | T. Desmarez P. Brat L. Lassois B. Barral O. Hubert Assessing banana stalk susceptibility to pathogens and their virulence MethodsX Assessing banana stalk susceptibility to pathogens and their virulence |
| title | Assessing banana stalk susceptibility to pathogens and their virulence |
| title_full | Assessing banana stalk susceptibility to pathogens and their virulence |
| title_fullStr | Assessing banana stalk susceptibility to pathogens and their virulence |
| title_full_unstemmed | Assessing banana stalk susceptibility to pathogens and their virulence |
| title_short | Assessing banana stalk susceptibility to pathogens and their virulence |
| title_sort | assessing banana stalk susceptibility to pathogens and their virulence |
| topic | Assessing banana stalk susceptibility to pathogens and their virulence |
| url | http://www.sciencedirect.com/science/article/pii/S2215016125000901 |
| work_keys_str_mv | AT tdesmarez assessingbananastalksusceptibilitytopathogensandtheirvirulence AT pbrat assessingbananastalksusceptibilitytopathogensandtheirvirulence AT llassois assessingbananastalksusceptibilitytopathogensandtheirvirulence AT bbarral assessingbananastalksusceptibilitytopathogensandtheirvirulence AT ohubert assessingbananastalksusceptibilitytopathogensandtheirvirulence |