Sesamum Indicum Gel: A Natural Foe to Candida Albicans

Background: Oral candidiasis is a common fungal infection typically treated with antifungal drugs, which may cause side effects. Herbal treatments have emerged as a potential natural alternative with fewer adverse effects. Objective: This exploratory study aimed to evaluate the antifungal potential...

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Main Authors: Keerthana Selvam, Suman J. Lakshmi, Senthil Kumar Balasubramanian
Format: Article
Language:English
Published: Wolters Kluwer Medknow Publications 2025-04-01
Series:Journal of Indian Academy of Oral Medicine and Radiology
Subjects:
Online Access:https://journals.lww.com/10.4103/jiaomr.jiaomr_35_25
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author Keerthana Selvam
Suman J. Lakshmi
Senthil Kumar Balasubramanian
author_facet Keerthana Selvam
Suman J. Lakshmi
Senthil Kumar Balasubramanian
author_sort Keerthana Selvam
collection DOAJ
description Background: Oral candidiasis is a common fungal infection typically treated with antifungal drugs, which may cause side effects. Herbal treatments have emerged as a potential natural alternative with fewer adverse effects. Objective: This exploratory study aimed to evaluate the antifungal potential of a gel formulated using the most effective extract of Sesamum indicum root. Objectives included identifying the effective solvent, assessing antifungal activity via the well diffusion method, analyzing active compounds through GC-MS, and evaluating the antifungal activity of the formulated gel. Methods: Root extracts were prepared by macerating Sesamum indicum powder in ethanol, water, and hexane. Different amounts of root powder (10, 15, 20, and 25 grams) were mixed with 100 mL of ethanol to determine the most effective concentration. The extract showing the highest antifungal activity was analyzed using GC-MS to identify bioactive compounds. A gel was then formulated and tested in vitro for antifungal efficacy. Due to the exploratory nature of the study, a limited sample size was used to generate preliminary findings. Results: The ethanol extract exhibited the highest antifungal activity. GC-MS identified key compounds including (S)-(+)-2-Amino-3-methyl-1-butanol, 1,3-Propanediol, 2-(hydroxymethyl)-2-nitro, and 2,4-Di-tert-butyl-phenol. The herbal gel showed a 10.03 mm zone of inhibition, compared to 11.25 mm for clotrimazole, with statistically significant results. Conclusion: The formulated herbal gel demonstrated promising antifungal activity comparable to clotrimazole. However, the small sample size and preliminary nature of this study warrant further research to confirm efficacy, safety, and clinical applicability.
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institution Kabale University
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spelling doaj-art-e8c59725dcbb4b7d9ea8f65632c3108a2025-08-20T03:28:55ZengWolters Kluwer Medknow PublicationsJournal of Indian Academy of Oral Medicine and Radiology0972-13630975-15722025-04-0137215616210.4103/jiaomr.jiaomr_35_25Sesamum Indicum Gel: A Natural Foe to Candida AlbicansKeerthana SelvamSuman J. LakshmiSenthil Kumar BalasubramanianBackground: Oral candidiasis is a common fungal infection typically treated with antifungal drugs, which may cause side effects. Herbal treatments have emerged as a potential natural alternative with fewer adverse effects. Objective: This exploratory study aimed to evaluate the antifungal potential of a gel formulated using the most effective extract of Sesamum indicum root. Objectives included identifying the effective solvent, assessing antifungal activity via the well diffusion method, analyzing active compounds through GC-MS, and evaluating the antifungal activity of the formulated gel. Methods: Root extracts were prepared by macerating Sesamum indicum powder in ethanol, water, and hexane. Different amounts of root powder (10, 15, 20, and 25 grams) were mixed with 100 mL of ethanol to determine the most effective concentration. The extract showing the highest antifungal activity was analyzed using GC-MS to identify bioactive compounds. A gel was then formulated and tested in vitro for antifungal efficacy. Due to the exploratory nature of the study, a limited sample size was used to generate preliminary findings. Results: The ethanol extract exhibited the highest antifungal activity. GC-MS identified key compounds including (S)-(+)-2-Amino-3-methyl-1-butanol, 1,3-Propanediol, 2-(hydroxymethyl)-2-nitro, and 2,4-Di-tert-butyl-phenol. The herbal gel showed a 10.03 mm zone of inhibition, compared to 11.25 mm for clotrimazole, with statistically significant results. Conclusion: The formulated herbal gel demonstrated promising antifungal activity comparable to clotrimazole. However, the small sample size and preliminary nature of this study warrant further research to confirm efficacy, safety, and clinical applicability.https://journals.lww.com/10.4103/jiaomr.jiaomr_35_25antifungal agentscandida albicanschromatographygelsmass spectrometrysesamum indicum
spellingShingle Keerthana Selvam
Suman J. Lakshmi
Senthil Kumar Balasubramanian
Sesamum Indicum Gel: A Natural Foe to Candida Albicans
Journal of Indian Academy of Oral Medicine and Radiology
antifungal agents
candida albicans
chromatography
gels
mass spectrometry
sesamum indicum
title Sesamum Indicum Gel: A Natural Foe to Candida Albicans
title_full Sesamum Indicum Gel: A Natural Foe to Candida Albicans
title_fullStr Sesamum Indicum Gel: A Natural Foe to Candida Albicans
title_full_unstemmed Sesamum Indicum Gel: A Natural Foe to Candida Albicans
title_short Sesamum Indicum Gel: A Natural Foe to Candida Albicans
title_sort sesamum indicum gel a natural foe to candida albicans
topic antifungal agents
candida albicans
chromatography
gels
mass spectrometry
sesamum indicum
url https://journals.lww.com/10.4103/jiaomr.jiaomr_35_25
work_keys_str_mv AT keerthanaselvam sesamumindicumgelanaturalfoetocandidaalbicans
AT sumanjlakshmi sesamumindicumgelanaturalfoetocandidaalbicans
AT senthilkumarbalasubramanian sesamumindicumgelanaturalfoetocandidaalbicans