Di (2-ethylhexyl) phthalate reduces sperm motility by decreasing sperm tail energy supply
Di (2-ethylhexyl) phthalate (DEHP) is widespread in the environment. It can impair sperm function through damaging the sperm development process. However, few studies have focused on the sperm tail that is directly related to sperm motility. In this study, we demonstrate that DEHP impedes the conver...
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Elsevier
2025-01-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651325001472 |
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| author | Xiao-lu Li Qi-meng Li Yuan-zhuo Zheng Die Hu Xiao-yue Cai Ke Yin Yin-yin Qi Zi yu Cheng Xia Ning Yang Cai Wei Wu Ting-yuan Lin Tao Xu Ling-li Zhao |
| author_facet | Xiao-lu Li Qi-meng Li Yuan-zhuo Zheng Die Hu Xiao-yue Cai Ke Yin Yin-yin Qi Zi yu Cheng Xia Ning Yang Cai Wei Wu Ting-yuan Lin Tao Xu Ling-li Zhao |
| author_sort | Xiao-lu Li |
| collection | DOAJ |
| description | Di (2-ethylhexyl) phthalate (DEHP) is widespread in the environment. It can impair sperm function through damaging the sperm development process. However, few studies have focused on the sperm tail that is directly related to sperm motility. In this study, we demonstrate that DEHP impedes the conversion of riboflavin in the mitochondrial sheath in the midsection of the sperm tail, resulting in reduced ATP (adenosine triphosphate) synthesis, thereby inhibiting sperm motility. The results of HPLC-MS/MS showed that DEHP metabolites were transported to the testes and epididium, indicating that MEHP (Mono-2-ethylexyl phthalate) could directly affect the espermatozoa of mature. Sperm motility analysis determined that sperm motility decreased with increasing DEHP concentration. The movement of sperm is mainly dependent on the tail motility, which is largely determined by tail structure and energy supply. Electron microscopy images illustrate that there are no observable changes in the basic kinematic structure of the sperm tail. However, DEHP causes a decrease in complex II activity of mitochondrial respiratory chain by interfering with the synthesis of the cofactor FAD (Flavin adenine dinucleotide), which leads to a decrease in ATP concentration. Therefore, DEHP exposure can reduce sperm motility by decreasing sperm tail energy supply. This study exemplifies the importance of the sperm tail in sperm dysfunction caused by environmental pollutants. |
| format | Article |
| id | doaj-art-e8c4c2f3ac08482a9cffd2cd38e324a7 |
| institution | Kabale University |
| issn | 0147-6513 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj-art-e8c4c2f3ac08482a9cffd2cd38e324a72025-02-12T05:30:22ZengElsevierEcotoxicology and Environmental Safety0147-65132025-01-01290117811Di (2-ethylhexyl) phthalate reduces sperm motility by decreasing sperm tail energy supplyXiao-lu Li0Qi-meng Li1Yuan-zhuo Zheng2Die Hu3Xiao-yue Cai4Ke Yin5Yin-yin Qi6Zi yu Cheng7Xia Ning8Yang Cai9Wei Wu10Ting-yuan Lin11Tao Xu12Ling-li Zhao13Department of Toxicology, School of Public Health; Suzhou Hospital of Anhui Medical University, Hefei 230032, China; Key Laboratory of Environmental Toxicology of Anhui Higher Education Institutes, Hefei 230032, ChinaDepartment of Toxicology, School of Public Health; Suzhou Hospital of Anhui Medical University, Hefei 230032, China; Key Laboratory of Environmental Toxicology of Anhui Higher Education Institutes, Hefei 230032, ChinaDepartment of Toxicology, School of Public Health; Suzhou Hospital of Anhui Medical University, Hefei 230032, China; Key Laboratory of Environmental Toxicology of Anhui Higher Education Institutes, Hefei 230032, ChinaDepartment of Toxicology, School of Public Health; Suzhou Hospital of Anhui Medical University, Hefei 230032, China; Key Laboratory of Environmental Toxicology of Anhui Higher Education Institutes, Hefei 230032, ChinaDepartment of Toxicology, School of Public Health; Suzhou Hospital of Anhui Medical University, Hefei 230032, China; Key Laboratory of Environmental Toxicology of Anhui Higher Education Institutes, Hefei 230032, ChinaDepartment of Toxicology, School of Public Health; Suzhou Hospital of Anhui Medical University, Hefei 230032, China; Key Laboratory of Environmental Toxicology of Anhui Higher Education Institutes, Hefei 230032, ChinaDepartment of Toxicology, School of Public Health; Suzhou Hospital of Anhui Medical University, Hefei 230032, China; Key Laboratory of Environmental Toxicology of Anhui Higher Education Institutes, Hefei 230032, ChinaSchool of Biology, Food and Environment, Hefei University, Hefei 230601, ChinaDepartment of Toxicology, School of Public Health; Suzhou Hospital of Anhui Medical University, Hefei 230032, China; Key Laboratory of Environmental Toxicology of Anhui Higher Education Institutes, Hefei 230032, ChinaDepartment of Toxicology, School of Public Health; Suzhou Hospital of Anhui Medical University, Hefei 230032, China; Key Laboratory of Environmental Toxicology of Anhui Higher Education Institutes, Hefei 230032, ChinaDepartment of Toxicology, School of Public Health; Suzhou Hospital of Anhui Medical University, Hefei 230032, China; Key Laboratory of Environmental Toxicology of Anhui Higher Education Institutes, Hefei 230032, ChinaDepartment of Toxicology, School of Public Health; Suzhou Hospital of Anhui Medical University, Hefei 230032, China; Key Laboratory of Environmental Toxicology of Anhui Higher Education Institutes, Hefei 230032, ChinaSchool of Biology, Food and Environment, Hefei University, Hefei 230601, China; Corresponding author.Department of Toxicology, School of Public Health; Suzhou Hospital of Anhui Medical University, Hefei 230032, China; Key Laboratory of Environmental Toxicology of Anhui Higher Education Institutes, Hefei 230032, China; Corresponding author at: Department of Toxicology, School of Public Health; Suzhou Hospital of Anhui Medical University, Hefei 230032, China.Di (2-ethylhexyl) phthalate (DEHP) is widespread in the environment. It can impair sperm function through damaging the sperm development process. However, few studies have focused on the sperm tail that is directly related to sperm motility. In this study, we demonstrate that DEHP impedes the conversion of riboflavin in the mitochondrial sheath in the midsection of the sperm tail, resulting in reduced ATP (adenosine triphosphate) synthesis, thereby inhibiting sperm motility. The results of HPLC-MS/MS showed that DEHP metabolites were transported to the testes and epididium, indicating that MEHP (Mono-2-ethylexyl phthalate) could directly affect the espermatozoa of mature. Sperm motility analysis determined that sperm motility decreased with increasing DEHP concentration. The movement of sperm is mainly dependent on the tail motility, which is largely determined by tail structure and energy supply. Electron microscopy images illustrate that there are no observable changes in the basic kinematic structure of the sperm tail. However, DEHP causes a decrease in complex II activity of mitochondrial respiratory chain by interfering with the synthesis of the cofactor FAD (Flavin adenine dinucleotide), which leads to a decrease in ATP concentration. Therefore, DEHP exposure can reduce sperm motility by decreasing sperm tail energy supply. This study exemplifies the importance of the sperm tail in sperm dysfunction caused by environmental pollutants.http://www.sciencedirect.com/science/article/pii/S0147651325001472Di (2-ethylhexyl) phthalate (DEHP)Motility of spermSperm tailFADRiboflavin |
| spellingShingle | Xiao-lu Li Qi-meng Li Yuan-zhuo Zheng Die Hu Xiao-yue Cai Ke Yin Yin-yin Qi Zi yu Cheng Xia Ning Yang Cai Wei Wu Ting-yuan Lin Tao Xu Ling-li Zhao Di (2-ethylhexyl) phthalate reduces sperm motility by decreasing sperm tail energy supply Ecotoxicology and Environmental Safety Di (2-ethylhexyl) phthalate (DEHP) Motility of sperm Sperm tail FAD Riboflavin |
| title | Di (2-ethylhexyl) phthalate reduces sperm motility by decreasing sperm tail energy supply |
| title_full | Di (2-ethylhexyl) phthalate reduces sperm motility by decreasing sperm tail energy supply |
| title_fullStr | Di (2-ethylhexyl) phthalate reduces sperm motility by decreasing sperm tail energy supply |
| title_full_unstemmed | Di (2-ethylhexyl) phthalate reduces sperm motility by decreasing sperm tail energy supply |
| title_short | Di (2-ethylhexyl) phthalate reduces sperm motility by decreasing sperm tail energy supply |
| title_sort | di 2 ethylhexyl phthalate reduces sperm motility by decreasing sperm tail energy supply |
| topic | Di (2-ethylhexyl) phthalate (DEHP) Motility of sperm Sperm tail FAD Riboflavin |
| url | http://www.sciencedirect.com/science/article/pii/S0147651325001472 |
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