Effects of N-Carbamylglutamate supplementation on cecal morphology, microbiota composition, and short-chain fatty acids contents of broiler breeder roosters
Abstract The objective of this study was to assess the effects of N-Carbamylglutamate (NCG) supplementation on cecal morphology, microbiota composition, and short-chain fatty acids (SCFAs) contents in broiler breeder roosters. A total of 72 11-week-old Zhuanghe Dagu broiler breeder roosters with a s...
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Nature Portfolio
2025-03-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-025-91577-w |
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| author | Na Liu Zhe Zhang Jiehui Zhang Wei Ma Chunqiang Wang |
| author_facet | Na Liu Zhe Zhang Jiehui Zhang Wei Ma Chunqiang Wang |
| author_sort | Na Liu |
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| description | Abstract The objective of this study was to assess the effects of N-Carbamylglutamate (NCG) supplementation on cecal morphology, microbiota composition, and short-chain fatty acids (SCFAs) contents in broiler breeder roosters. A total of 72 11-week-old Zhuanghe Dagu broiler breeder roosters with a similar initial body weight (1.53 ± 0.06 kg) were randomly allocated into two groups. Each group had 3 replicates with 12 birds per replicate. The experimental period lasted 42 days. All birds underwent the same production practices, except for the dietary conditions. It was found that an increase in cecal muscularis thickness and villi epithelium thickness. The NCG supplementation was found to have regulatory effects on the composition of cecal microbiota. Additionally, the study observed an increase in the content of butyric acid in the cecum of broiler breeder roosters fed with the NCG-containing control diet compared to those fed with the basal diet. Spearman correlation analysis showed that the variation of cecal microbiota was closely related to the production of butyric acid as well as the improvement of muscularis and villi epithelium thickness in cecum. The increase of butyric acid content in cecum was positively correlated with the improvement of cecal muscularis and villi epithelium thickness. In conclusion, the findings of this study indicate that dietary supplementation of NCG in broiler breeder roosters can positively influence cecal morphology, microbiota composition, and butyric production. |
| format | Article |
| id | doaj-art-e8c18c184fcb4a00bf2fa77772100b18 |
| institution | DOAJ |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-03-01 |
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| spelling | doaj-art-e8c18c184fcb4a00bf2fa77772100b182025-08-20T02:59:20ZengNature PortfolioScientific Reports2045-23222025-03-0115111210.1038/s41598-025-91577-wEffects of N-Carbamylglutamate supplementation on cecal morphology, microbiota composition, and short-chain fatty acids contents of broiler breeder roostersNa Liu0Zhe Zhang1Jiehui Zhang2Wei Ma3Chunqiang Wang4College of Animal Science and Veterinary Medicine, Jinzhou Medical UniversityCollege of Animal Science and Veterinary Medicine, Jinzhou Medical UniversityCollege of Animal Science and Veterinary Medicine, Jinzhou Medical UniversityCollege of Animal Science and Veterinary Medicine, Jinzhou Medical UniversityCollege of Animal Science and Veterinary Medicine, Jinzhou Medical UniversityAbstract The objective of this study was to assess the effects of N-Carbamylglutamate (NCG) supplementation on cecal morphology, microbiota composition, and short-chain fatty acids (SCFAs) contents in broiler breeder roosters. A total of 72 11-week-old Zhuanghe Dagu broiler breeder roosters with a similar initial body weight (1.53 ± 0.06 kg) were randomly allocated into two groups. Each group had 3 replicates with 12 birds per replicate. The experimental period lasted 42 days. All birds underwent the same production practices, except for the dietary conditions. It was found that an increase in cecal muscularis thickness and villi epithelium thickness. The NCG supplementation was found to have regulatory effects on the composition of cecal microbiota. Additionally, the study observed an increase in the content of butyric acid in the cecum of broiler breeder roosters fed with the NCG-containing control diet compared to those fed with the basal diet. Spearman correlation analysis showed that the variation of cecal microbiota was closely related to the production of butyric acid as well as the improvement of muscularis and villi epithelium thickness in cecum. The increase of butyric acid content in cecum was positively correlated with the improvement of cecal muscularis and villi epithelium thickness. In conclusion, the findings of this study indicate that dietary supplementation of NCG in broiler breeder roosters can positively influence cecal morphology, microbiota composition, and butyric production.https://doi.org/10.1038/s41598-025-91577-wN-CarbamylglutamateBroiler chickIntestinal healthIntestinal microbiotaHindgut fermentation |
| spellingShingle | Na Liu Zhe Zhang Jiehui Zhang Wei Ma Chunqiang Wang Effects of N-Carbamylglutamate supplementation on cecal morphology, microbiota composition, and short-chain fatty acids contents of broiler breeder roosters Scientific Reports N-Carbamylglutamate Broiler chick Intestinal health Intestinal microbiota Hindgut fermentation |
| title | Effects of N-Carbamylglutamate supplementation on cecal morphology, microbiota composition, and short-chain fatty acids contents of broiler breeder roosters |
| title_full | Effects of N-Carbamylglutamate supplementation on cecal morphology, microbiota composition, and short-chain fatty acids contents of broiler breeder roosters |
| title_fullStr | Effects of N-Carbamylglutamate supplementation on cecal morphology, microbiota composition, and short-chain fatty acids contents of broiler breeder roosters |
| title_full_unstemmed | Effects of N-Carbamylglutamate supplementation on cecal morphology, microbiota composition, and short-chain fatty acids contents of broiler breeder roosters |
| title_short | Effects of N-Carbamylglutamate supplementation on cecal morphology, microbiota composition, and short-chain fatty acids contents of broiler breeder roosters |
| title_sort | effects of n carbamylglutamate supplementation on cecal morphology microbiota composition and short chain fatty acids contents of broiler breeder roosters |
| topic | N-Carbamylglutamate Broiler chick Intestinal health Intestinal microbiota Hindgut fermentation |
| url | https://doi.org/10.1038/s41598-025-91577-w |
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