MicroRNA editing facilitates immune elimination of HCMV infected cells.
The human cytomegalovirus (HCMV) is extremely prevalent in the human population. Infection by HCMV is life threatening in immune compromised individuals and in immune competent individuals it can cause severe birth defects, developmental retardation and is even associated with tumor development. Whi...
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| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-02-01
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| Series: | PLoS Pathogens |
| Online Access: | https://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.1003963&type=printable |
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| _version_ | 1850022918485966848 |
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| author | Daphna Nachmani Albert Zimmermann Esther Oiknine Djian Yiska Weisblum Yoav Livneh Vu Thuy Khanh Le Eithan Galun Vaclav Horejsi Ofer Isakov Noam Shomron Dana G Wolf Hartmut Hengel Ofer Mandelboim |
| author_facet | Daphna Nachmani Albert Zimmermann Esther Oiknine Djian Yiska Weisblum Yoav Livneh Vu Thuy Khanh Le Eithan Galun Vaclav Horejsi Ofer Isakov Noam Shomron Dana G Wolf Hartmut Hengel Ofer Mandelboim |
| author_sort | Daphna Nachmani |
| collection | DOAJ |
| description | The human cytomegalovirus (HCMV) is extremely prevalent in the human population. Infection by HCMV is life threatening in immune compromised individuals and in immune competent individuals it can cause severe birth defects, developmental retardation and is even associated with tumor development. While numerous mechanisms were developed by HCMV to interfere with immune cell activity, much less is known about cellular mechanisms that operate in response to HCMV infection. Here we demonstrate that in response to HCMV infection, the expression of the short form of the RNA editing enzyme ADAR1 (ADAR1-p110) is induced. We identified the specific promoter region responsible for this induction and we show that ADAR1-p110 can edit miR-376a. Accordingly, we demonstrate that the levels of the edited-miR-376a (miR-376a(e)) increase during HCMV infection. Importantly, we show that miR-376a(e) downregulates the immune modulating molecule HLA-E and that this consequently renders HCMV infected cells susceptible to elimination by NK cells. |
| format | Article |
| id | doaj-art-e8c1418cb98640ef9ed5e405b2cb6b43 |
| institution | DOAJ |
| issn | 1553-7366 1553-7374 |
| language | English |
| publishDate | 2014-02-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Pathogens |
| spelling | doaj-art-e8c1418cb98640ef9ed5e405b2cb6b432025-08-20T03:01:31ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742014-02-01102e100396310.1371/journal.ppat.1003963MicroRNA editing facilitates immune elimination of HCMV infected cells.Daphna NachmaniAlbert ZimmermannEsther Oiknine DjianYiska WeisblumYoav LivnehVu Thuy Khanh LeEithan GalunVaclav HorejsiOfer IsakovNoam ShomronDana G WolfHartmut HengelOfer MandelboimThe human cytomegalovirus (HCMV) is extremely prevalent in the human population. Infection by HCMV is life threatening in immune compromised individuals and in immune competent individuals it can cause severe birth defects, developmental retardation and is even associated with tumor development. While numerous mechanisms were developed by HCMV to interfere with immune cell activity, much less is known about cellular mechanisms that operate in response to HCMV infection. Here we demonstrate that in response to HCMV infection, the expression of the short form of the RNA editing enzyme ADAR1 (ADAR1-p110) is induced. We identified the specific promoter region responsible for this induction and we show that ADAR1-p110 can edit miR-376a. Accordingly, we demonstrate that the levels of the edited-miR-376a (miR-376a(e)) increase during HCMV infection. Importantly, we show that miR-376a(e) downregulates the immune modulating molecule HLA-E and that this consequently renders HCMV infected cells susceptible to elimination by NK cells.https://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.1003963&type=printable |
| spellingShingle | Daphna Nachmani Albert Zimmermann Esther Oiknine Djian Yiska Weisblum Yoav Livneh Vu Thuy Khanh Le Eithan Galun Vaclav Horejsi Ofer Isakov Noam Shomron Dana G Wolf Hartmut Hengel Ofer Mandelboim MicroRNA editing facilitates immune elimination of HCMV infected cells. PLoS Pathogens |
| title | MicroRNA editing facilitates immune elimination of HCMV infected cells. |
| title_full | MicroRNA editing facilitates immune elimination of HCMV infected cells. |
| title_fullStr | MicroRNA editing facilitates immune elimination of HCMV infected cells. |
| title_full_unstemmed | MicroRNA editing facilitates immune elimination of HCMV infected cells. |
| title_short | MicroRNA editing facilitates immune elimination of HCMV infected cells. |
| title_sort | microrna editing facilitates immune elimination of hcmv infected cells |
| url | https://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.1003963&type=printable |
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