Role and mechanism of pumilio homolog 1 in hypoxia/reoxygenation induced injury of human renal tubular epithelial cells
Objective To explore the role and mechanism of pumilio homolog 1 （PUM1） in hypoxia/reoxygenation induced cell injury in human renal tubular epithelial cells （HK-2）. Methods A hypoxia/reoxygenation （H/R） model of HK-2 cells was established in vitro. PUM1 expression was knocked down through small inte...
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Editorial Department of Journal of Clinical Nephrology
2024-10-01
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| Series: | Linchuang shenzangbing zazhi |
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| Online Access: | http://www.lcszb.com/cn/article/doi/10.3969/j.issn.1671-2390.2024.10.007 |
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| author | Sheng-guo Hu Yi Guo Chao Yuan You-kong Li Min Wang Min Zhu |
| author_facet | Sheng-guo Hu Yi Guo Chao Yuan You-kong Li Min Wang Min Zhu |
| author_sort | Sheng-guo Hu |
| collection | DOAJ |
| description | Objective To explore the role and mechanism of pumilio homolog 1 （PUM1） in hypoxia/reoxygenation induced cell injury in human renal tubular epithelial cells （HK-2）. Methods A hypoxia/reoxygenation （H/R） model of HK-2 cells was established in vitro. PUM1 expression was knocked down through small interfering RNA （siRNA）. Cells were randomized into three groups of control， hypoxia/reoxygenation （H/R） and H/R+siRNA. Western blot （WB） method was used for detecting the expression level of PUM1 protein. Cell Count Kit 8 （CCK-8） was employed for detecting cell viability. Hydrogen peroxide （H2O2）， malondialdehyde （MDA） and superoxide dismutase （SOD） were used for evaluating the levels of oxidative stress. Flow cytometry was utilized for detecting the level of cell apoptosis. Results As compared with control group， protein expression level of PUM1 in H/R 3 h group （1.76 ± 0.11 vs 0.98 ± 0.05）， H/R 6 h group （2.89 ± 0.14 vs 0.98 ± 0.05） and H/R 12 h group （3.78 ± 0.08 vs 0.98 ± 0.05） gradually spiked with the prolongation of hypoxic time. As compared with H/R group， knocking down the expression of PUM1 significantly improved the cell viability （73.67 ± 3.42 vs 29.60 ± 2.94）， oxidative stress [H2O2:（13.53 ± 0.85）μmol/L vs （22.43 ± 1.12）μmol/L， MDA: （16.03 ± 0.70）μmol/L vs （31.20 ± 1.50）μmol/L， SOD: （34670 ± 1800）U/L vs （5730 ± 1220）U/L] and apoptotic level [（14.89 ± 1.65）% vs （39.71 ± 1.94）%] after H/R in H/R+si-PUM1 group. Conclusion PUM1 is up-regulated in H/R induced HK-2 cells and its inhibition may alleviate H/R injury through reducing oxidative stress and lowering cell apoptosis levels. |
| format | Article |
| id | doaj-art-e8c022a3527f468ab1f819a59ab9245d |
| institution | OA Journals |
| issn | 1671-2390 |
| language | zho |
| publishDate | 2024-10-01 |
| publisher | Editorial Department of Journal of Clinical Nephrology |
| record_format | Article |
| series | Linchuang shenzangbing zazhi |
| spelling | doaj-art-e8c022a3527f468ab1f819a59ab9245d2025-08-20T01:47:54ZzhoEditorial Department of Journal of Clinical NephrologyLinchuang shenzangbing zazhi1671-23902024-10-01241084184610.3969/j.issn.1671-2390.2024.10.00720230447Role and mechanism of pumilio homolog 1 in hypoxia/reoxygenation induced injury of human renal tubular epithelial cellsSheng-guo Hu0Yi Guo1Chao Yuan2You-kong Li3Min Wang4Min Zhu5Department of Urology, Jingzhou Hospital Affiliated to Yangtze University, Jingzhou 434020, ChinaDepartment of Urology, Jingzhou Hospital Affiliated to Yangtze University, Jingzhou 434020, ChinaDepartment of Urology, Jingzhou Hospital Affiliated to Yangtze University, Jingzhou 434020, ChinaDepartment of Urology, Jingzhou Hospital Affiliated to Yangtze University, Jingzhou 434020, ChinaDepartment of Urology, Renmin Hospital of Wuhan University, Wuhan 430060, ChinaDepartment of Urology, Jingzhou Hospital Affiliated to Yangtze University, Jingzhou 434020, ChinaObjective To explore the role and mechanism of pumilio homolog 1 （PUM1） in hypoxia/reoxygenation induced cell injury in human renal tubular epithelial cells （HK-2）. Methods A hypoxia/reoxygenation （H/R） model of HK-2 cells was established in vitro. PUM1 expression was knocked down through small interfering RNA （siRNA）. Cells were randomized into three groups of control， hypoxia/reoxygenation （H/R） and H/R+siRNA. Western blot （WB） method was used for detecting the expression level of PUM1 protein. Cell Count Kit 8 （CCK-8） was employed for detecting cell viability. Hydrogen peroxide （H2O2）， malondialdehyde （MDA） and superoxide dismutase （SOD） were used for evaluating the levels of oxidative stress. Flow cytometry was utilized for detecting the level of cell apoptosis. Results As compared with control group， protein expression level of PUM1 in H/R 3 h group （1.76 ± 0.11 vs 0.98 ± 0.05）， H/R 6 h group （2.89 ± 0.14 vs 0.98 ± 0.05） and H/R 12 h group （3.78 ± 0.08 vs 0.98 ± 0.05） gradually spiked with the prolongation of hypoxic time. As compared with H/R group， knocking down the expression of PUM1 significantly improved the cell viability （73.67 ± 3.42 vs 29.60 ± 2.94）， oxidative stress [H2O2:（13.53 ± 0.85）μmol/L vs （22.43 ± 1.12）μmol/L， MDA: （16.03 ± 0.70）μmol/L vs （31.20 ± 1.50）μmol/L， SOD: （34670 ± 1800）U/L vs （5730 ± 1220）U/L] and apoptotic level [（14.89 ± 1.65）% vs （39.71 ± 1.94）%] after H/R in H/R+si-PUM1 group. Conclusion PUM1 is up-regulated in H/R induced HK-2 cells and its inhibition may alleviate H/R injury through reducing oxidative stress and lowering cell apoptosis levels.http://www.lcszb.com/cn/article/doi/10.3969/j.issn.1671-2390.2024.10.007pumilio homolog 1human kidney-2hypoxia/reoxygenationoxidative stressapoptosis |
| spellingShingle | Sheng-guo Hu Yi Guo Chao Yuan You-kong Li Min Wang Min Zhu Role and mechanism of pumilio homolog 1 in hypoxia/reoxygenation induced injury of human renal tubular epithelial cells Linchuang shenzangbing zazhi pumilio homolog 1 human kidney-2 hypoxia/reoxygenation oxidative stress apoptosis |
| title | Role and mechanism of pumilio homolog 1 in hypoxia/reoxygenation induced injury of human renal tubular epithelial cells |
| title_full | Role and mechanism of pumilio homolog 1 in hypoxia/reoxygenation induced injury of human renal tubular epithelial cells |
| title_fullStr | Role and mechanism of pumilio homolog 1 in hypoxia/reoxygenation induced injury of human renal tubular epithelial cells |
| title_full_unstemmed | Role and mechanism of pumilio homolog 1 in hypoxia/reoxygenation induced injury of human renal tubular epithelial cells |
| title_short | Role and mechanism of pumilio homolog 1 in hypoxia/reoxygenation induced injury of human renal tubular epithelial cells |
| title_sort | role and mechanism of pumilio homolog 1 in hypoxia reoxygenation induced injury of human renal tubular epithelial cells |
| topic | pumilio homolog 1 human kidney-2 hypoxia/reoxygenation oxidative stress apoptosis |
| url | http://www.lcszb.com/cn/article/doi/10.3969/j.issn.1671-2390.2024.10.007 |
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