Calanquinone A suppresses glioma progression via STAT3-mediated regulation of c-Myc and MMP9
Abstract Calanthe arisanensis, a traditional medicinal orchid, contains Calanquinone A, a quinone-type compound with reported anticancer properties. Glioblastoma multiforme (GBM) is a highly aggressive brain tumor with limited treatment options and a poor prognosis. This study investigates the molec...
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| Format: | Article |
| Language: | English |
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Springer
2025-08-01
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| Series: | Discover Oncology |
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| Online Access: | https://doi.org/10.1007/s12672-025-03279-4 |
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| author | Wen-Chi Hsieh Chun-Yu Lin Hsuan‐Cheng Wu Huang-Wei Lo Chiung-Yuan Ko Jian-Ying Chuang Tsung-I. Hsu Tsui-Hwa Tseng Teng-Wei Huang Shao-Ming Wang |
| author_facet | Wen-Chi Hsieh Chun-Yu Lin Hsuan‐Cheng Wu Huang-Wei Lo Chiung-Yuan Ko Jian-Ying Chuang Tsung-I. Hsu Tsui-Hwa Tseng Teng-Wei Huang Shao-Ming Wang |
| author_sort | Wen-Chi Hsieh |
| collection | DOAJ |
| description | Abstract Calanthe arisanensis, a traditional medicinal orchid, contains Calanquinone A, a quinone-type compound with reported anticancer properties. Glioblastoma multiforme (GBM) is a highly aggressive brain tumor with limited treatment options and a poor prognosis. This study investigates the molecular mechanisms underlying the antiproliferative and antimigratory effects of Calanquinone A in GBM. Human GBM cell lines (U87MG) and patient-derived primary GBM cells (Pt#3) were treated with Calanquinone A (1–2.5 µM), and effects on cell viability, proliferation, and migration were assessed using CCK-8, trypan blue exclusion, colony formation, wound healing, transwell migration, and F-actin staining assays. Calanquinone A significantly reduced GBM cell viability and migration. Western blot analysis revealed that Calanquinone A inhibited STAT3 phosphorylation at Tyr705, leading to downregulation of its downstream targets c-Myc and MMP9. STAT3 overexpression reversed these effects, confirming the involvement of STAT3-dependent pathways. In a mouse xenograft model, Calanquinone A suppressed tumor formation in vivo. Molecular docking analysis further revealed a potential direct interaction between Calanquinone A and STAT3, supporting a direct mechanism of action. These findings demonstrate that Calanquinone A exerts potent antitumor effects in glioblastoma by targeting the STAT3/c-Myc and STAT3/MMP9 signaling axes, supporting its potential as a natural STAT3-targeting agent for GBM therapy. Graphical abstract |
| format | Article |
| id | doaj-art-e8bbc0cba6324424b62eaea78744dfbb |
| institution | Kabale University |
| issn | 2730-6011 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Springer |
| record_format | Article |
| series | Discover Oncology |
| spelling | doaj-art-e8bbc0cba6324424b62eaea78744dfbb2025-08-20T03:43:11ZengSpringerDiscover Oncology2730-60112025-08-0116111910.1007/s12672-025-03279-4Calanquinone A suppresses glioma progression via STAT3-mediated regulation of c-Myc and MMP9Wen-Chi Hsieh0Chun-Yu Lin1Hsuan‐Cheng Wu2Huang-Wei Lo3Chiung-Yuan Ko4Jian-Ying Chuang5Tsung-I. Hsu6Tsui-Hwa Tseng7Teng-Wei Huang8Shao-Ming Wang9Neuroscience and Brain Disease Center, China Medical UniversityNeuroscience and Brain Disease Center, China Medical UniversityNeuroscience and Brain Disease Center, China Medical UniversityNeuroscience and Brain Disease Center, China Medical UniversitySchool of Medicine, College of Medicine, National Sun Yat-sen UniversityPh.D. Program in Medical Neuroscience, College of Medical Science and Technology, Taipei Medical UniversityPh.D. Program in Medical Neuroscience, College of Medical Science and Technology, Taipei Medical UniversityDepartment of Medical Applied Chemistry, Chung Shan Medical UniversityNeuroscience and Brain Disease Center, China Medical UniversityNeuroscience and Brain Disease Center, China Medical UniversityAbstract Calanthe arisanensis, a traditional medicinal orchid, contains Calanquinone A, a quinone-type compound with reported anticancer properties. Glioblastoma multiforme (GBM) is a highly aggressive brain tumor with limited treatment options and a poor prognosis. This study investigates the molecular mechanisms underlying the antiproliferative and antimigratory effects of Calanquinone A in GBM. Human GBM cell lines (U87MG) and patient-derived primary GBM cells (Pt#3) were treated with Calanquinone A (1–2.5 µM), and effects on cell viability, proliferation, and migration were assessed using CCK-8, trypan blue exclusion, colony formation, wound healing, transwell migration, and F-actin staining assays. Calanquinone A significantly reduced GBM cell viability and migration. Western blot analysis revealed that Calanquinone A inhibited STAT3 phosphorylation at Tyr705, leading to downregulation of its downstream targets c-Myc and MMP9. STAT3 overexpression reversed these effects, confirming the involvement of STAT3-dependent pathways. In a mouse xenograft model, Calanquinone A suppressed tumor formation in vivo. Molecular docking analysis further revealed a potential direct interaction between Calanquinone A and STAT3, supporting a direct mechanism of action. These findings demonstrate that Calanquinone A exerts potent antitumor effects in glioblastoma by targeting the STAT3/c-Myc and STAT3/MMP9 signaling axes, supporting its potential as a natural STAT3-targeting agent for GBM therapy. Graphical abstracthttps://doi.org/10.1007/s12672-025-03279-4Calanthe arisanensisCalanquinone aPhytochemicalGlioblastoma multiformeSTAT3Migration |
| spellingShingle | Wen-Chi Hsieh Chun-Yu Lin Hsuan‐Cheng Wu Huang-Wei Lo Chiung-Yuan Ko Jian-Ying Chuang Tsung-I. Hsu Tsui-Hwa Tseng Teng-Wei Huang Shao-Ming Wang Calanquinone A suppresses glioma progression via STAT3-mediated regulation of c-Myc and MMP9 Discover Oncology Calanthe arisanensis Calanquinone a Phytochemical Glioblastoma multiforme STAT3 Migration |
| title | Calanquinone A suppresses glioma progression via STAT3-mediated regulation of c-Myc and MMP9 |
| title_full | Calanquinone A suppresses glioma progression via STAT3-mediated regulation of c-Myc and MMP9 |
| title_fullStr | Calanquinone A suppresses glioma progression via STAT3-mediated regulation of c-Myc and MMP9 |
| title_full_unstemmed | Calanquinone A suppresses glioma progression via STAT3-mediated regulation of c-Myc and MMP9 |
| title_short | Calanquinone A suppresses glioma progression via STAT3-mediated regulation of c-Myc and MMP9 |
| title_sort | calanquinone a suppresses glioma progression via stat3 mediated regulation of c myc and mmp9 |
| topic | Calanthe arisanensis Calanquinone a Phytochemical Glioblastoma multiforme STAT3 Migration |
| url | https://doi.org/10.1007/s12672-025-03279-4 |
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