EGCG and Taxifolin Modulate Secretory Activity and Expression of Dentinogenesis Markers in Odontoblast-Like Cells
Odontoblasts are cells specialized in dentin matrix deposition and the first line of defense when the dentin–pulp complex is injured by pathological processes, such as dental caries and trauma. Natural compounds, such as flavonoids, could be useful to stimulate odontoblast activity and reparative de...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2025-01-01
|
| Series: | Scientifica |
| Online Access: | http://dx.doi.org/10.1155/sci5/3233536 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849729634002796544 |
|---|---|
| author | Cristiane Duque Rafaela Laruzo Rabelo Gabriela Pacheco de Almeida Braga Igor Paulino Mendes Soares Maria Eduarda de Souza Daniela Alvim Chrisostomo Josimeri Hebling Carlos Alberto de Souza Costa Anuradha Prakki Luís Octavio Regasini |
| author_facet | Cristiane Duque Rafaela Laruzo Rabelo Gabriela Pacheco de Almeida Braga Igor Paulino Mendes Soares Maria Eduarda de Souza Daniela Alvim Chrisostomo Josimeri Hebling Carlos Alberto de Souza Costa Anuradha Prakki Luís Octavio Regasini |
| author_sort | Cristiane Duque |
| collection | DOAJ |
| description | Odontoblasts are cells specialized in dentin matrix deposition and the first line of defense when the dentin–pulp complex is injured by pathological processes, such as dental caries and trauma. Natural compounds, such as flavonoids, could be useful to stimulate odontoblast activity and reparative dentinogenesis in vital pulp therapies, especially in immature permanent teeth. This study evaluated the effect of flavonoids on odontoblast secretory activity and the expression of dentinogenesis markers. The effect of flavonoids was evaluated on phenotypic mineralization markers (alkaline phosphatase (ALP) activity and mineralized nodule deposition) by colorimetric assays and on the expression of Alpl, Mmp2, Mmp9, Dmp1, and Dspp genes in odontoblast-like cells by quantitative polymerase chain reaction. Most of the flavonoids did not show toxicity between 100 and 25 μM. In distinct concentrations, epigallocatechin gallate (EGCG), taxifolin, myricetin, quercetin, and kaempferol stimulated the activity of ALP and increased mineralized nodule deposition. However, the highest effect on those phenotypic markers was observed after EGCG and taxifolin treatments. Then, they were selected for evaluation of gene expression. mRNA levels of Dmp1 and Dspp highly increased with taxifolin treatment, and Alpl expression was increased for both taxifolin and EGCG groups, without difference between them. Mmp2 and Mmp9 expression was not affected by these flavonoids. In conclusion, EGCG and taxifolin positively affect phenotypic mineralization markers; in particular, taxifolin highly stimulates early- and late-stage dentinogenesis genes. |
| format | Article |
| id | doaj-art-e8bb67af6ed844fcbafc1b40dfa6b932 |
| institution | DOAJ |
| issn | 2090-908X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Scientifica |
| spelling | doaj-art-e8bb67af6ed844fcbafc1b40dfa6b9322025-08-20T03:09:09ZengWileyScientifica2090-908X2025-01-01202510.1155/sci5/3233536EGCG and Taxifolin Modulate Secretory Activity and Expression of Dentinogenesis Markers in Odontoblast-Like CellsCristiane Duque0Rafaela Laruzo Rabelo1Gabriela Pacheco de Almeida Braga2Igor Paulino Mendes Soares3Maria Eduarda de Souza4Daniela Alvim Chrisostomo5Josimeri Hebling6Carlos Alberto de Souza Costa7Anuradha Prakki8Luís Octavio Regasini9Universidade Católica Portuguesa (UCP)São Paulo State University (UNESP)São Paulo State University (UNESP)São Paulo State University (UNESP)São Paulo State University (UNESP)São Paulo State University (UNESP)São Paulo State University (UNESP)São Paulo State University (UNESP)University of TorontoSão Paulo State University (UNESP)Odontoblasts are cells specialized in dentin matrix deposition and the first line of defense when the dentin–pulp complex is injured by pathological processes, such as dental caries and trauma. Natural compounds, such as flavonoids, could be useful to stimulate odontoblast activity and reparative dentinogenesis in vital pulp therapies, especially in immature permanent teeth. This study evaluated the effect of flavonoids on odontoblast secretory activity and the expression of dentinogenesis markers. The effect of flavonoids was evaluated on phenotypic mineralization markers (alkaline phosphatase (ALP) activity and mineralized nodule deposition) by colorimetric assays and on the expression of Alpl, Mmp2, Mmp9, Dmp1, and Dspp genes in odontoblast-like cells by quantitative polymerase chain reaction. Most of the flavonoids did not show toxicity between 100 and 25 μM. In distinct concentrations, epigallocatechin gallate (EGCG), taxifolin, myricetin, quercetin, and kaempferol stimulated the activity of ALP and increased mineralized nodule deposition. However, the highest effect on those phenotypic markers was observed after EGCG and taxifolin treatments. Then, they were selected for evaluation of gene expression. mRNA levels of Dmp1 and Dspp highly increased with taxifolin treatment, and Alpl expression was increased for both taxifolin and EGCG groups, without difference between them. Mmp2 and Mmp9 expression was not affected by these flavonoids. In conclusion, EGCG and taxifolin positively affect phenotypic mineralization markers; in particular, taxifolin highly stimulates early- and late-stage dentinogenesis genes.http://dx.doi.org/10.1155/sci5/3233536 |
| spellingShingle | Cristiane Duque Rafaela Laruzo Rabelo Gabriela Pacheco de Almeida Braga Igor Paulino Mendes Soares Maria Eduarda de Souza Daniela Alvim Chrisostomo Josimeri Hebling Carlos Alberto de Souza Costa Anuradha Prakki Luís Octavio Regasini EGCG and Taxifolin Modulate Secretory Activity and Expression of Dentinogenesis Markers in Odontoblast-Like Cells Scientifica |
| title | EGCG and Taxifolin Modulate Secretory Activity and Expression of Dentinogenesis Markers in Odontoblast-Like Cells |
| title_full | EGCG and Taxifolin Modulate Secretory Activity and Expression of Dentinogenesis Markers in Odontoblast-Like Cells |
| title_fullStr | EGCG and Taxifolin Modulate Secretory Activity and Expression of Dentinogenesis Markers in Odontoblast-Like Cells |
| title_full_unstemmed | EGCG and Taxifolin Modulate Secretory Activity and Expression of Dentinogenesis Markers in Odontoblast-Like Cells |
| title_short | EGCG and Taxifolin Modulate Secretory Activity and Expression of Dentinogenesis Markers in Odontoblast-Like Cells |
| title_sort | egcg and taxifolin modulate secretory activity and expression of dentinogenesis markers in odontoblast like cells |
| url | http://dx.doi.org/10.1155/sci5/3233536 |
| work_keys_str_mv | AT cristianeduque egcgandtaxifolinmodulatesecretoryactivityandexpressionofdentinogenesismarkersinodontoblastlikecells AT rafaelalaruzorabelo egcgandtaxifolinmodulatesecretoryactivityandexpressionofdentinogenesismarkersinodontoblastlikecells AT gabrielapachecodealmeidabraga egcgandtaxifolinmodulatesecretoryactivityandexpressionofdentinogenesismarkersinodontoblastlikecells AT igorpaulinomendessoares egcgandtaxifolinmodulatesecretoryactivityandexpressionofdentinogenesismarkersinodontoblastlikecells AT mariaeduardadesouza egcgandtaxifolinmodulatesecretoryactivityandexpressionofdentinogenesismarkersinodontoblastlikecells AT danielaalvimchrisostomo egcgandtaxifolinmodulatesecretoryactivityandexpressionofdentinogenesismarkersinodontoblastlikecells AT josimerihebling egcgandtaxifolinmodulatesecretoryactivityandexpressionofdentinogenesismarkersinodontoblastlikecells AT carlosalbertodesouzacosta egcgandtaxifolinmodulatesecretoryactivityandexpressionofdentinogenesismarkersinodontoblastlikecells AT anuradhaprakki egcgandtaxifolinmodulatesecretoryactivityandexpressionofdentinogenesismarkersinodontoblastlikecells AT luisoctavioregasini egcgandtaxifolinmodulatesecretoryactivityandexpressionofdentinogenesismarkersinodontoblastlikecells |