Preparation, in vitro release and antibacterial activity evaluation of rifampicin and moxifloxacin-loaded poly(D,L-lactide-co-glycolide) microspheres
Osteomyelitis is difficult to treat because infective bone is poorly accessible for intravenously administering antibiotics and biofilm formation increases bacterial resistance. In this study, microspheres prepared using poly(lactide-co-glycolide) (PLGA) and embedded with moxifloxacin (MOX–PLGA micr...
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Taylor & Francis Group
2019-12-01
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| Series: | Artificial Cells, Nanomedicine, and Biotechnology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/21691401.2019.1581792 |
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| author | ZeWen Qiao Zhi Yuan Wenping Zhang Daihao Wei Ningmin Hu |
| author_facet | ZeWen Qiao Zhi Yuan Wenping Zhang Daihao Wei Ningmin Hu |
| author_sort | ZeWen Qiao |
| collection | DOAJ |
| description | Osteomyelitis is difficult to treat because infective bone is poorly accessible for intravenously administering antibiotics and biofilm formation increases bacterial resistance. In this study, microspheres prepared using poly(lactide-co-glycolide) (PLGA) and embedded with moxifloxacin (MOX–PLGA microspheres) and rifampicin/moxifloxacin (RIF/MOX–PLGA microspheres) using the water-in-oil-in-water double emulsion solvent evaporation technique were used for local delivery. Shape of MOX–PLGA microspheres and RIF/MOX–PLGA microspheres were spherical, mean particle size of them were 20.52 μm and 16.62 μm, respectively. Encapsulation efficiency of the MOX-PLGA microspheres was 17.35% ± 2.42%. However, the encapsulation efficiency for MOX and RIF in RIF/MOX–PLGA microspheres was 33.25% ± 7.51% and 49.0% ± 11.25%, respectively. Moxifloxacin and rifampicin were released slowly from microspheres. Both microspheres can efficiently release antibiotics in vitro. Antibacterial and bacterial biofilm-inhibition properties of the released solution were investigated from RIF/MOX–PLGA, MOX–PLGA, and blank PLGA microspheres at varying time points in vitro. RIF/MOX–PLGA microspheres demonstrated the strongest antibacterial activity and bacterial biofilm-inhibition property than the other two microspheres (p < .05). This study suggests that the novel RIF/MOX–PLGA microspheres can be used as a promising carrier for osteomyelitis treatment. |
| format | Article |
| id | doaj-art-e8b9112a67934310af0da37bd99494bb |
| institution | Kabale University |
| issn | 2169-1401 2169-141X |
| language | English |
| publishDate | 2019-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Artificial Cells, Nanomedicine, and Biotechnology |
| spelling | doaj-art-e8b9112a67934310af0da37bd99494bb2025-08-20T03:51:25ZengTaylor & Francis GroupArtificial Cells, Nanomedicine, and Biotechnology2169-14012169-141X2019-12-0147179079810.1080/21691401.2019.1581792Preparation, in vitro release and antibacterial activity evaluation of rifampicin and moxifloxacin-loaded poly(D,L-lactide-co-glycolide) microspheresZeWen Qiao0Zhi Yuan1Wenping Zhang2Daihao Wei3Ningmin Hu4Department of Orthopedics, Xijing Hospital, Fourth Military Medical University, Xi'an, ChinaDepartment of Orthopedics, Xijing Hospital, Fourth Military Medical University, Xi'an, ChinaDepartment of Pharmacy, Institute of Clinical Pharmacology, General Hospital of Ningxia Medical University, Yinchuan, ChinaGeneral Hospital of Ningxia Medical University, Yinchuan, ChinaAffiliated General Hospital, Ningxia Medical University, Yinchuan, ChinaOsteomyelitis is difficult to treat because infective bone is poorly accessible for intravenously administering antibiotics and biofilm formation increases bacterial resistance. In this study, microspheres prepared using poly(lactide-co-glycolide) (PLGA) and embedded with moxifloxacin (MOX–PLGA microspheres) and rifampicin/moxifloxacin (RIF/MOX–PLGA microspheres) using the water-in-oil-in-water double emulsion solvent evaporation technique were used for local delivery. Shape of MOX–PLGA microspheres and RIF/MOX–PLGA microspheres were spherical, mean particle size of them were 20.52 μm and 16.62 μm, respectively. Encapsulation efficiency of the MOX-PLGA microspheres was 17.35% ± 2.42%. However, the encapsulation efficiency for MOX and RIF in RIF/MOX–PLGA microspheres was 33.25% ± 7.51% and 49.0% ± 11.25%, respectively. Moxifloxacin and rifampicin were released slowly from microspheres. Both microspheres can efficiently release antibiotics in vitro. Antibacterial and bacterial biofilm-inhibition properties of the released solution were investigated from RIF/MOX–PLGA, MOX–PLGA, and blank PLGA microspheres at varying time points in vitro. RIF/MOX–PLGA microspheres demonstrated the strongest antibacterial activity and bacterial biofilm-inhibition property than the other two microspheres (p < .05). This study suggests that the novel RIF/MOX–PLGA microspheres can be used as a promising carrier for osteomyelitis treatment.https://www.tandfonline.com/doi/10.1080/21691401.2019.1581792Bacterial biofilmosteomyelitisPLGA microspheresmoxifloxacinrifampicin |
| spellingShingle | ZeWen Qiao Zhi Yuan Wenping Zhang Daihao Wei Ningmin Hu Preparation, in vitro release and antibacterial activity evaluation of rifampicin and moxifloxacin-loaded poly(D,L-lactide-co-glycolide) microspheres Artificial Cells, Nanomedicine, and Biotechnology Bacterial biofilm osteomyelitis PLGA microspheres moxifloxacin rifampicin |
| title | Preparation, in vitro release and antibacterial activity evaluation of rifampicin and moxifloxacin-loaded poly(D,L-lactide-co-glycolide) microspheres |
| title_full | Preparation, in vitro release and antibacterial activity evaluation of rifampicin and moxifloxacin-loaded poly(D,L-lactide-co-glycolide) microspheres |
| title_fullStr | Preparation, in vitro release and antibacterial activity evaluation of rifampicin and moxifloxacin-loaded poly(D,L-lactide-co-glycolide) microspheres |
| title_full_unstemmed | Preparation, in vitro release and antibacterial activity evaluation of rifampicin and moxifloxacin-loaded poly(D,L-lactide-co-glycolide) microspheres |
| title_short | Preparation, in vitro release and antibacterial activity evaluation of rifampicin and moxifloxacin-loaded poly(D,L-lactide-co-glycolide) microspheres |
| title_sort | preparation in vitro release and antibacterial activity evaluation of rifampicin and moxifloxacin loaded poly d l lactide co glycolide microspheres |
| topic | Bacterial biofilm osteomyelitis PLGA microspheres moxifloxacin rifampicin |
| url | https://www.tandfonline.com/doi/10.1080/21691401.2019.1581792 |
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