<i>Exserohilum rostratum</i>-Mediated Synthesis of Silver Nanoparticles: A Case Study on Their Bioherbicidal Activity Against <i>Leptochloa chinensis</i> (L.) Nees
The interdisciplinary progress in nanotechnology has yielded environmentally friendly and cost-effective strategies to enhance bioherbicidal efficacy. This study presents the biosynthesis of silver nanoparticles (M-AgNPs) using the fungus <i>Exserohilum rostratum</i>, specifically target...
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MDPI AG
2024-11-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/14/12/2784 |
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| author | Ashrit Gulfraz Yuquan Yuan Qing Bu Muhammad Shafiq Zhiqiu Huang Mingwei Li Zhaoxia Dong Jing An Yong Chen |
| author_facet | Ashrit Gulfraz Yuquan Yuan Qing Bu Muhammad Shafiq Zhiqiu Huang Mingwei Li Zhaoxia Dong Jing An Yong Chen |
| author_sort | Ashrit Gulfraz |
| collection | DOAJ |
| description | The interdisciplinary progress in nanotechnology has yielded environmentally friendly and cost-effective strategies to enhance bioherbicidal efficacy. This study presents the biosynthesis of silver nanoparticles (M-AgNPs) using the fungus <i>Exserohilum rostratum</i>, specifically targeting the <i>Leptochloa chinensis</i> weed in paddy fields. The M-AgNPs were characterized with an aqueous solution size of 107.9 nm and a zeta potential of −24.0 ± 0.20 mV, and their properties were analyzed by UV-Vis spectrophotometry, transmission electron microscopy (TEM), scanning electron microscopy (SEM), X-ray diffraction (XRD), and Fourier-transform infrared spectroscopy (FTIR). The application of M-AgNP suspension at different concentrations of 70 µg∙mL<sup>−1</sup>, 80 µg∙mL<sup>−1</sup>, and 100 µg∙mL<sup>−1</sup> to <i>L. chinensis</i> at the 3–4 leaf stage resulted in significant herbicidal effects. These nanoparticles induced oxidative stress and significantly reduced the activities of peroxidase, catalase, and superoxide dismutase in the weed seedlings. Meanwhile, M-AgNP treatments significantly increased the activity of cell wall-degrading enzymes, including polygalacturonase and cellulase, in <i>L. chinensis</i> leaves and caused organelle damage in plant leaf cells. Safety assessments showed no significant impact on rice growth after treatment with M-AgNP<sub>3</sub> (100 µg∙mL<sup>−1</sup>) suspension. Our results suggest that M-AgNPs represent a sustainable and eco-friendly approach to weed control that is compatible with rice cultivation, thus supporting the adoption of green agricultural practices. |
| format | Article |
| id | doaj-art-e8b0022ccfe142f6aa745784917e997a |
| institution | DOAJ |
| issn | 2073-4395 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj-art-e8b0022ccfe142f6aa745784917e997a2025-08-20T02:55:49ZengMDPI AGAgronomy2073-43952024-11-011412278410.3390/agronomy14122784<i>Exserohilum rostratum</i>-Mediated Synthesis of Silver Nanoparticles: A Case Study on Their Bioherbicidal Activity Against <i>Leptochloa chinensis</i> (L.) NeesAshrit Gulfraz0Yuquan Yuan1Qing Bu2Muhammad Shafiq3Zhiqiu Huang4Mingwei Li5Zhaoxia Dong6Jing An7Yong Chen8College of Agriculture, South China Agricultural University, Guangzhou 510642, ChinaCollege of Agriculture, South China Agricultural University, Guangzhou 510642, ChinaCollege of Agriculture, South China Agricultural University, Guangzhou 510642, ChinaResearch Institute of Clinical Pharmacy, Department of Pharmacology, Shantou University Medical College, Shantou 515041, ChinaCollege of Agriculture, South China Agricultural University, Guangzhou 510642, ChinaCollege of Agriculture, South China Agricultural University, Guangzhou 510642, ChinaCollege of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, ChinaCollege of Agriculture, South China Agricultural University, Guangzhou 510642, ChinaCollege of Agriculture, South China Agricultural University, Guangzhou 510642, ChinaThe interdisciplinary progress in nanotechnology has yielded environmentally friendly and cost-effective strategies to enhance bioherbicidal efficacy. This study presents the biosynthesis of silver nanoparticles (M-AgNPs) using the fungus <i>Exserohilum rostratum</i>, specifically targeting the <i>Leptochloa chinensis</i> weed in paddy fields. The M-AgNPs were characterized with an aqueous solution size of 107.9 nm and a zeta potential of −24.0 ± 0.20 mV, and their properties were analyzed by UV-Vis spectrophotometry, transmission electron microscopy (TEM), scanning electron microscopy (SEM), X-ray diffraction (XRD), and Fourier-transform infrared spectroscopy (FTIR). The application of M-AgNP suspension at different concentrations of 70 µg∙mL<sup>−1</sup>, 80 µg∙mL<sup>−1</sup>, and 100 µg∙mL<sup>−1</sup> to <i>L. chinensis</i> at the 3–4 leaf stage resulted in significant herbicidal effects. These nanoparticles induced oxidative stress and significantly reduced the activities of peroxidase, catalase, and superoxide dismutase in the weed seedlings. Meanwhile, M-AgNP treatments significantly increased the activity of cell wall-degrading enzymes, including polygalacturonase and cellulase, in <i>L. chinensis</i> leaves and caused organelle damage in plant leaf cells. Safety assessments showed no significant impact on rice growth after treatment with M-AgNP<sub>3</sub> (100 µg∙mL<sup>−1</sup>) suspension. Our results suggest that M-AgNPs represent a sustainable and eco-friendly approach to weed control that is compatible with rice cultivation, thus supporting the adoption of green agricultural practices.https://www.mdpi.com/2073-4395/14/12/2784mycosynthesized silver nanoparticles<i>Exserohilum rostratum</i>herbicidal activity<i>Leptochloa chinensis</i> (L.) Neesreactive oxygen speciesrice safety |
| spellingShingle | Ashrit Gulfraz Yuquan Yuan Qing Bu Muhammad Shafiq Zhiqiu Huang Mingwei Li Zhaoxia Dong Jing An Yong Chen <i>Exserohilum rostratum</i>-Mediated Synthesis of Silver Nanoparticles: A Case Study on Their Bioherbicidal Activity Against <i>Leptochloa chinensis</i> (L.) Nees Agronomy mycosynthesized silver nanoparticles <i>Exserohilum rostratum</i> herbicidal activity <i>Leptochloa chinensis</i> (L.) Nees reactive oxygen species rice safety |
| title | <i>Exserohilum rostratum</i>-Mediated Synthesis of Silver Nanoparticles: A Case Study on Their Bioherbicidal Activity Against <i>Leptochloa chinensis</i> (L.) Nees |
| title_full | <i>Exserohilum rostratum</i>-Mediated Synthesis of Silver Nanoparticles: A Case Study on Their Bioherbicidal Activity Against <i>Leptochloa chinensis</i> (L.) Nees |
| title_fullStr | <i>Exserohilum rostratum</i>-Mediated Synthesis of Silver Nanoparticles: A Case Study on Their Bioherbicidal Activity Against <i>Leptochloa chinensis</i> (L.) Nees |
| title_full_unstemmed | <i>Exserohilum rostratum</i>-Mediated Synthesis of Silver Nanoparticles: A Case Study on Their Bioherbicidal Activity Against <i>Leptochloa chinensis</i> (L.) Nees |
| title_short | <i>Exserohilum rostratum</i>-Mediated Synthesis of Silver Nanoparticles: A Case Study on Their Bioherbicidal Activity Against <i>Leptochloa chinensis</i> (L.) Nees |
| title_sort | i exserohilum rostratum i mediated synthesis of silver nanoparticles a case study on their bioherbicidal activity against i leptochloa chinensis i l nees |
| topic | mycosynthesized silver nanoparticles <i>Exserohilum rostratum</i> herbicidal activity <i>Leptochloa chinensis</i> (L.) Nees reactive oxygen species rice safety |
| url | https://www.mdpi.com/2073-4395/14/12/2784 |
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