Reusing waste biomass in crop protection—Calcinated oyster shell powder enhances rhizospheric microbial-mediated suppression of root-knot nematodes
Root-knot nematodes (RKNs), particularly Meloidogyne incognita, are one of the most destructive plant-parasitic nematodes (PPNs) affecting crop production worldwide. Previous earlier study revealed that calcinated oyster shell powder (OSP) possessed excellent suppression of tobacco RKN disease. Howe...
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Frontiers Media S.A.
2025-08-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1625653/full |
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| author | Qipeng Jiang Jiamin Yu Yong Wang Jinfeng Wang Lianqiang Jiang Shiping Guo Yu Qian Xiangwen Yu Dongyang Liu Daojiang Xi Quan Deng Wei Ding Shili Li |
| author_facet | Qipeng Jiang Jiamin Yu Yong Wang Jinfeng Wang Lianqiang Jiang Shiping Guo Yu Qian Xiangwen Yu Dongyang Liu Daojiang Xi Quan Deng Wei Ding Shili Li |
| author_sort | Qipeng Jiang |
| collection | DOAJ |
| description | Root-knot nematodes (RKNs), particularly Meloidogyne incognita, are one of the most destructive plant-parasitic nematodes (PPNs) affecting crop production worldwide. Previous earlier study revealed that calcinated oyster shell powder (OSP) possessed excellent suppression of tobacco RKN disease. However, the suppression mechanism of OSP against RKNs still remains unrevealed. Hence, this study aimed to clarify the suppression mechanism of oyster shell powder against RKNs by pot experiments and high-throughput sequencing. The results showed that calcinated OSP reduced over 38% of the tobacco root-knot index by inhibiting the migration of second-stage juveniles of Meloidogyne incognita (J2) in soil. Furthermore, calcinated OSP reduced J2 density by 43.69% in the tobacco rhizosphere, and significantly increased soil pH by 0.68; moreover, it increased the contents of soil exchangeable calcium (ExchCa) and exchangeable magnesium (ExchMg) by over 50%. Meanwhile, soil properties, including ExchMg, ExchCa, and pH, enhanced microbial-mediated suppression of J2. Specifically, some taxa within Proteobacteria- and Gemmatimonadota-dominated microbial communities and fungal richness may contribute to suppression of RKNs. Conversely, some taxa within Chloroflexi- and Acidobacteriota-dominated microbial communities may be involved in RKNs' prosperity. Our study suggests that reusing waste oyster shell powder as an innovative antagonist against RKNs presents promising avenues for nature-based PPN management strategies, and would generate significant economic value and social impact in plant protection. |
| format | Article |
| id | doaj-art-e8afc4e3bace4b9282ea72858de55e76 |
| institution | Kabale University |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj-art-e8afc4e3bace4b9282ea72858de55e762025-08-25T05:25:34ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-08-011610.3389/fmicb.2025.16256531625653Reusing waste biomass in crop protection—Calcinated oyster shell powder enhances rhizospheric microbial-mediated suppression of root-knot nematodesQipeng Jiang0Jiamin Yu1Yong Wang2Jinfeng Wang3Lianqiang Jiang4Shiping Guo5Yu Qian6Xiangwen Yu7Dongyang Liu8Daojiang Xi9Quan Deng10Wei Ding11Shili Li12College of Plant Protection, Southwest University, Chongqing, ChinaSichuan Branch of China National Tobacco Corporation, Chengdu, ChinaLiangshan Prefecture Branch of Sichuan Tobacco Corporation, Xichang, ChinaCollege of Plant Protection, Southwest University, Chongqing, ChinaLiangshan Prefecture Branch of Sichuan Tobacco Corporation, Xichang, ChinaSichuan Branch of China National Tobacco Corporation, Chengdu, ChinaSichuan Branch of China National Tobacco Corporation, Chengdu, ChinaSichuan Branch of China National Tobacco Corporation, Chengdu, ChinaLiangshan Prefecture Branch of Sichuan Tobacco Corporation, Xichang, ChinaCollege of Plant Protection, Southwest University, Chongqing, ChinaLiangshan Prefecture Branch of Sichuan Tobacco Corporation, Xichang, ChinaCollege of Plant Protection, Southwest University, Chongqing, ChinaCollege of Plant Protection, Southwest University, Chongqing, ChinaRoot-knot nematodes (RKNs), particularly Meloidogyne incognita, are one of the most destructive plant-parasitic nematodes (PPNs) affecting crop production worldwide. Previous earlier study revealed that calcinated oyster shell powder (OSP) possessed excellent suppression of tobacco RKN disease. However, the suppression mechanism of OSP against RKNs still remains unrevealed. Hence, this study aimed to clarify the suppression mechanism of oyster shell powder against RKNs by pot experiments and high-throughput sequencing. The results showed that calcinated OSP reduced over 38% of the tobacco root-knot index by inhibiting the migration of second-stage juveniles of Meloidogyne incognita (J2) in soil. Furthermore, calcinated OSP reduced J2 density by 43.69% in the tobacco rhizosphere, and significantly increased soil pH by 0.68; moreover, it increased the contents of soil exchangeable calcium (ExchCa) and exchangeable magnesium (ExchMg) by over 50%. Meanwhile, soil properties, including ExchMg, ExchCa, and pH, enhanced microbial-mediated suppression of J2. Specifically, some taxa within Proteobacteria- and Gemmatimonadota-dominated microbial communities and fungal richness may contribute to suppression of RKNs. Conversely, some taxa within Chloroflexi- and Acidobacteriota-dominated microbial communities may be involved in RKNs' prosperity. Our study suggests that reusing waste oyster shell powder as an innovative antagonist against RKNs presents promising avenues for nature-based PPN management strategies, and would generate significant economic value and social impact in plant protection.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1625653/fulloyster shell powderroot-knot nematodeMeloidogyne incognitamicrobial communitysoil amendments |
| spellingShingle | Qipeng Jiang Jiamin Yu Yong Wang Jinfeng Wang Lianqiang Jiang Shiping Guo Yu Qian Xiangwen Yu Dongyang Liu Daojiang Xi Quan Deng Wei Ding Shili Li Reusing waste biomass in crop protection—Calcinated oyster shell powder enhances rhizospheric microbial-mediated suppression of root-knot nematodes Frontiers in Microbiology oyster shell powder root-knot nematode Meloidogyne incognita microbial community soil amendments |
| title | Reusing waste biomass in crop protection—Calcinated oyster shell powder enhances rhizospheric microbial-mediated suppression of root-knot nematodes |
| title_full | Reusing waste biomass in crop protection—Calcinated oyster shell powder enhances rhizospheric microbial-mediated suppression of root-knot nematodes |
| title_fullStr | Reusing waste biomass in crop protection—Calcinated oyster shell powder enhances rhizospheric microbial-mediated suppression of root-knot nematodes |
| title_full_unstemmed | Reusing waste biomass in crop protection—Calcinated oyster shell powder enhances rhizospheric microbial-mediated suppression of root-knot nematodes |
| title_short | Reusing waste biomass in crop protection—Calcinated oyster shell powder enhances rhizospheric microbial-mediated suppression of root-knot nematodes |
| title_sort | reusing waste biomass in crop protection calcinated oyster shell powder enhances rhizospheric microbial mediated suppression of root knot nematodes |
| topic | oyster shell powder root-knot nematode Meloidogyne incognita microbial community soil amendments |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1625653/full |
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