Can interferon-inducible gene expression guide treatment? A prospective study in QuantiFERON-positive uveitis with undetermined cause
PURPOSE: The purpose of this study was to evaluate the utility of baseline interferon (IFN)-inducible gene expression as a prognostic biomarker for Anti-tubercular therapy (ATT) response in patients with undetermined cause of uveitis who tested positive for QuantiFERON-TB Gold (QFT-positive uveitis)...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2025-04-01
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| Series: | Taiwan Journal of Ophthalmology |
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| Online Access: | https://journals.lww.com/10.4103/tjo.TJO-D-25-00029 |
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| author | Rina La Distia Nora Mei Riasanti Ratna Sitompul Lukman Edwar Made Susiyanti Yulia Aziza Ikhwanuliman Putera Maria Valentina Wibawa Ulifna Alfiya Sifyana Muhammad Zakiy Waliyuddin Rachel Ethelind Beti Ernawati Dewi Heri Wibowo |
| author_facet | Rina La Distia Nora Mei Riasanti Ratna Sitompul Lukman Edwar Made Susiyanti Yulia Aziza Ikhwanuliman Putera Maria Valentina Wibawa Ulifna Alfiya Sifyana Muhammad Zakiy Waliyuddin Rachel Ethelind Beti Ernawati Dewi Heri Wibowo |
| author_sort | Rina La Distia Nora |
| collection | DOAJ |
| description | PURPOSE:
The purpose of this study was to evaluate the utility of baseline interferon (IFN)-inducible gene expression as a prognostic biomarker for Anti-tubercular therapy (ATT) response in patients with undetermined cause of uveitis who tested positive for QuantiFERON-TB Gold (QFT-positive uveitis).
METHODS:
This prospective cohort study included 17 QFT-positive uveitis patients at a tertiary uveitis center in Indonesia. Baseline and week 2 peripheral blood transcripts were evaluated through real time-quantitative polymerase chain reaction to assess the expression of 10 IFN-inducible genes (IRF7, IFIT2, STAT1, IL1B, MyD88, TLR8, FCGR1B, GBP1, UBE2L6, and SERPING1). Patients were stratified into clusters based on gene expression patterns. The primary outcome was complete resolution of uveitis at 6 months.
RESULTS:
Hierarchical clustering revealed two distinct groups. Patients with higher baseline expression of IFN genes (Cluster 2) were more likely to achieve complete uveitis resolution after ATT compared to those with lower expression levels (Cluster 1) (80% vs. 43%). Using a previously established IFN gene signature score (IGSS) cutoff (≥5.61), 82% of high-scoring patients showed complete resolution, compared to only 33% in the low-scoring group (P = 0.046). However, week 2 gene expression changes did not correlate with treatment response, indicating limited utility in monitoring disease activity or predicting long-term outcomes.
CONCLUSION:
Baseline, but not week 2, peripheral blood IFN-inducible gene expression may serve as a prognostic biomarker for stratifying QFT-positive uveitis patients through prediction of their response to treatment. Patients with higher baseline IGSS are more likely to require ATT to achieve uveitis resolution at 6-month follow-up. |
| format | Article |
| id | doaj-art-e8a952a1e5c04504b09a6b66162eb9c8 |
| institution | DOAJ |
| issn | 2211-5056 2211-5072 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Taiwan Journal of Ophthalmology |
| spelling | doaj-art-e8a952a1e5c04504b09a6b66162eb9c82025-08-20T02:44:09ZengWolters Kluwer Medknow PublicationsTaiwan Journal of Ophthalmology2211-50562211-50722025-04-0115225926910.4103/tjo.TJO-D-25-00029Can interferon-inducible gene expression guide treatment? A prospective study in QuantiFERON-positive uveitis with undetermined causeRina La Distia NoraMei RiasantiRatna SitompulLukman EdwarMade SusiyantiYulia AzizaIkhwanuliman PuteraMaria Valentina WibawaUlifna Alfiya SifyanaMuhammad Zakiy WaliyuddinRachel EthelindBeti Ernawati DewiHeri WibowoPURPOSE: The purpose of this study was to evaluate the utility of baseline interferon (IFN)-inducible gene expression as a prognostic biomarker for Anti-tubercular therapy (ATT) response in patients with undetermined cause of uveitis who tested positive for QuantiFERON-TB Gold (QFT-positive uveitis). METHODS: This prospective cohort study included 17 QFT-positive uveitis patients at a tertiary uveitis center in Indonesia. Baseline and week 2 peripheral blood transcripts were evaluated through real time-quantitative polymerase chain reaction to assess the expression of 10 IFN-inducible genes (IRF7, IFIT2, STAT1, IL1B, MyD88, TLR8, FCGR1B, GBP1, UBE2L6, and SERPING1). Patients were stratified into clusters based on gene expression patterns. The primary outcome was complete resolution of uveitis at 6 months. RESULTS: Hierarchical clustering revealed two distinct groups. Patients with higher baseline expression of IFN genes (Cluster 2) were more likely to achieve complete uveitis resolution after ATT compared to those with lower expression levels (Cluster 1) (80% vs. 43%). Using a previously established IFN gene signature score (IGSS) cutoff (≥5.61), 82% of high-scoring patients showed complete resolution, compared to only 33% in the low-scoring group (P = 0.046). However, week 2 gene expression changes did not correlate with treatment response, indicating limited utility in monitoring disease activity or predicting long-term outcomes. CONCLUSION: Baseline, but not week 2, peripheral blood IFN-inducible gene expression may serve as a prognostic biomarker for stratifying QFT-positive uveitis patients through prediction of their response to treatment. Patients with higher baseline IGSS are more likely to require ATT to achieve uveitis resolution at 6-month follow-up.https://journals.lww.com/10.4103/tjo.TJO-D-25-00029antituberculosis therapybiomarkersgene expressioninterferon-inducible gene expressionprecision medicinequantiferon-tb goldtuberculosisuveitis |
| spellingShingle | Rina La Distia Nora Mei Riasanti Ratna Sitompul Lukman Edwar Made Susiyanti Yulia Aziza Ikhwanuliman Putera Maria Valentina Wibawa Ulifna Alfiya Sifyana Muhammad Zakiy Waliyuddin Rachel Ethelind Beti Ernawati Dewi Heri Wibowo Can interferon-inducible gene expression guide treatment? A prospective study in QuantiFERON-positive uveitis with undetermined cause Taiwan Journal of Ophthalmology antituberculosis therapy biomarkers gene expression interferon-inducible gene expression precision medicine quantiferon-tb gold tuberculosis uveitis |
| title | Can interferon-inducible gene expression guide treatment? A prospective study in QuantiFERON-positive uveitis with undetermined cause |
| title_full | Can interferon-inducible gene expression guide treatment? A prospective study in QuantiFERON-positive uveitis with undetermined cause |
| title_fullStr | Can interferon-inducible gene expression guide treatment? A prospective study in QuantiFERON-positive uveitis with undetermined cause |
| title_full_unstemmed | Can interferon-inducible gene expression guide treatment? A prospective study in QuantiFERON-positive uveitis with undetermined cause |
| title_short | Can interferon-inducible gene expression guide treatment? A prospective study in QuantiFERON-positive uveitis with undetermined cause |
| title_sort | can interferon inducible gene expression guide treatment a prospective study in quantiferon positive uveitis with undetermined cause |
| topic | antituberculosis therapy biomarkers gene expression interferon-inducible gene expression precision medicine quantiferon-tb gold tuberculosis uveitis |
| url | https://journals.lww.com/10.4103/tjo.TJO-D-25-00029 |
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