Genome-wide identification of PEBP gene family in Hedychium coronarium
The Phosphatidylethanolamine-binding protein (PEBP) gene family plays a crucial role in plant growth and development, particularly in regulating flowering time and morphogenesis. However, the diversity, expression patterns, and functions of PEBP genes in Hedychium coronarium remain largely unexplore...
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Frontiers Media S.A.
2025-07-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2025.1482764/full |
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| author | Qin Wang Yiwei Zhou Yiwei Zhou Fang Wang Xinyue Li Yunyi Yu Rangcai Yu Yanping Fan |
| author_facet | Qin Wang Yiwei Zhou Yiwei Zhou Fang Wang Xinyue Li Yunyi Yu Rangcai Yu Yanping Fan |
| author_sort | Qin Wang |
| collection | DOAJ |
| description | The Phosphatidylethanolamine-binding protein (PEBP) gene family plays a crucial role in plant growth and development, particularly in regulating flowering time and morphogenesis. However, the diversity, expression patterns, and functions of PEBP genes in Hedychium coronarium remain largely unexplored. In this study, 14 HcPEBP genes were identified and classified into MFT, FT, and TFL1 subfamilies based on phylogenetic analysis. Intraspecific collinearity analysis revealed three collinear relationships within the HcPEBP gene family. Interspecific collinearity analysis across H. coronarium, rice, wild banana, and pineapple highlighted the evolutionary significance of specific PEBP genes. Motifs DPDxP and GxHR are conserved in HcPEBPs, which are essential for anion-binding activity. At the same position in the C-terminus, FT-likes contain the xGxGGR motif, while TFL1-likes possesses the TAARRR motif. 64.3% of HcPEBP genes consist of four exons and three introns. Promoter regions of HcPEBP genes are enriched with light-responsive elements, suggesting a primary response to light signals. Expression patterns analysis by qRT-PCR showed that seven FT-like genes are predominantly expressed in leaves, with increased expression during the transition from vegetative to reproductive growth. HcPEBP11, a FT-like gene, is highly expressed in inflorescence buds. Overexpression of HcPEBP11 in tobacco induced early flowering, confirming its role in promoting flowering. This study provides a foundation for further research on the biological functions of the PEBP gene family in H. coronarium and elucidates the role of HcPEBP11 in flowering regulation. |
| format | Article |
| id | doaj-art-e8a8a317561649a8b95c7a3fa9ee26a2 |
| institution | OA Journals |
| issn | 1664-462X |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Plant Science |
| spelling | doaj-art-e8a8a317561649a8b95c7a3fa9ee26a22025-08-20T02:36:07ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2025-07-011610.3389/fpls.2025.14827641482764Genome-wide identification of PEBP gene family in Hedychium coronariumQin Wang0Yiwei Zhou1Yiwei Zhou2Fang Wang3Xinyue Li4Yunyi Yu5Rangcai Yu6Yanping Fan7The Research Center for Ornamental Plants, College of Horticulture, South China Agricultural University, Guangzhou, ChinaThe Research Center for Ornamental Plants, College of Horticulture, South China Agricultural University, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Ornamental Plant Germplasm Innovation and Utilization, Environmental Horticulture Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, ChinaThe Research Center for Ornamental Plants, College of Horticulture, South China Agricultural University, Guangzhou, ChinaThe Research Center for Ornamental Plants, College of Horticulture, South China Agricultural University, Guangzhou, ChinaThe Research Center for Ornamental Plants, College of Horticulture, South China Agricultural University, Guangzhou, ChinaCollege of Life Sciences, South China Agricultural University, Guangzhou, ChinaThe Research Center for Ornamental Plants, College of Horticulture, South China Agricultural University, Guangzhou, ChinaThe Phosphatidylethanolamine-binding protein (PEBP) gene family plays a crucial role in plant growth and development, particularly in regulating flowering time and morphogenesis. However, the diversity, expression patterns, and functions of PEBP genes in Hedychium coronarium remain largely unexplored. In this study, 14 HcPEBP genes were identified and classified into MFT, FT, and TFL1 subfamilies based on phylogenetic analysis. Intraspecific collinearity analysis revealed three collinear relationships within the HcPEBP gene family. Interspecific collinearity analysis across H. coronarium, rice, wild banana, and pineapple highlighted the evolutionary significance of specific PEBP genes. Motifs DPDxP and GxHR are conserved in HcPEBPs, which are essential for anion-binding activity. At the same position in the C-terminus, FT-likes contain the xGxGGR motif, while TFL1-likes possesses the TAARRR motif. 64.3% of HcPEBP genes consist of four exons and three introns. Promoter regions of HcPEBP genes are enriched with light-responsive elements, suggesting a primary response to light signals. Expression patterns analysis by qRT-PCR showed that seven FT-like genes are predominantly expressed in leaves, with increased expression during the transition from vegetative to reproductive growth. HcPEBP11, a FT-like gene, is highly expressed in inflorescence buds. Overexpression of HcPEBP11 in tobacco induced early flowering, confirming its role in promoting flowering. This study provides a foundation for further research on the biological functions of the PEBP gene family in H. coronarium and elucidates the role of HcPEBP11 in flowering regulation.https://www.frontiersin.org/articles/10.3389/fpls.2025.1482764/fullHedychium coronariumPEBP gene familyflowering regulationHcPEBP11expression patterns |
| spellingShingle | Qin Wang Yiwei Zhou Yiwei Zhou Fang Wang Xinyue Li Yunyi Yu Rangcai Yu Yanping Fan Genome-wide identification of PEBP gene family in Hedychium coronarium Frontiers in Plant Science Hedychium coronarium PEBP gene family flowering regulation HcPEBP11 expression patterns |
| title | Genome-wide identification of PEBP gene family in Hedychium coronarium |
| title_full | Genome-wide identification of PEBP gene family in Hedychium coronarium |
| title_fullStr | Genome-wide identification of PEBP gene family in Hedychium coronarium |
| title_full_unstemmed | Genome-wide identification of PEBP gene family in Hedychium coronarium |
| title_short | Genome-wide identification of PEBP gene family in Hedychium coronarium |
| title_sort | genome wide identification of pebp gene family in hedychium coronarium |
| topic | Hedychium coronarium PEBP gene family flowering regulation HcPEBP11 expression patterns |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2025.1482764/full |
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