3′-End cDNA Pool Suitable for Differential Display from a Small Number of Cells
We have generated a 3′ cDNA pool from the RNA of only 1000 or fewer cells by reverse transcription (RT) from an extended oligo(dT) primer with a 3′ degenerate base and a second strand primer with four degenerate 3′bases, followed by PCR. Reproducible differential displays (DD) can be made from this...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
1998-05-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/98245rr01 |
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