Dietary inulin supplementation modifies significantly the liver transcriptomic profile of broiler chickens.
Inclusion of prebiotics in the diet is known to be advantageous, with positive influences both on health and growth. The current study investigated the differences in the hepatic transcriptome profiles between chickens supplemented with inulin (a storage carbohydrate found in many plants) and contro...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0098942&type=printable |
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| author | Natalia Sevane Federica Bialade Susana Velasco Almudena Rebolé Maria Luisa Rodríguez Luís T Ortiz Javier Cañón Susana Dunner |
| author_facet | Natalia Sevane Federica Bialade Susana Velasco Almudena Rebolé Maria Luisa Rodríguez Luís T Ortiz Javier Cañón Susana Dunner |
| author_sort | Natalia Sevane |
| collection | DOAJ |
| description | Inclusion of prebiotics in the diet is known to be advantageous, with positive influences both on health and growth. The current study investigated the differences in the hepatic transcriptome profiles between chickens supplemented with inulin (a storage carbohydrate found in many plants) and controls. Liver is a major metabolic organ and has been previously reported to be involved in the modification of the lipid metabolism in chickens fed with inulin. A nutrigenomic approach through the analysis of liver RNA hybridized to the Affymetrix GeneChip Chicken Genome Array identified 148 differentially expressed genes among both groups: 104 up-regulated (≥ 1.4-fold) and 44 down-regulated (≤ 0.6-fold). Quantitative real-time PCR analysis validated the microarray expression results for five out of seven genes tested. The functional annotation analyses revealed a number of genes, processes and pathways with putative involvement in chicken growth and performance, while reinforcing the immune status of animals, and fostering the production of long chain fatty acids in broilers supplemented with 5 g of inulin kg(-1) diet. As far as we are aware, this is the first report of a microarray based gene expression study on the effect of dietary inulin supplementation, supporting further research on the use of this prebiotic on chicken diets as a useful alternative to antibiotics for improving performance and general immunity in poultry farming, along with a healthier meat lipid profile. |
| format | Article |
| id | doaj-art-e89e7663b4d048748e9a1329af4f857a |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-e89e7663b4d048748e9a1329af4f857a2025-08-20T03:01:19ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0196e9894210.1371/journal.pone.0098942Dietary inulin supplementation modifies significantly the liver transcriptomic profile of broiler chickens.Natalia SevaneFederica BialadeSusana VelascoAlmudena ReboléMaria Luisa RodríguezLuís T OrtizJavier CañónSusana DunnerInclusion of prebiotics in the diet is known to be advantageous, with positive influences both on health and growth. The current study investigated the differences in the hepatic transcriptome profiles between chickens supplemented with inulin (a storage carbohydrate found in many plants) and controls. Liver is a major metabolic organ and has been previously reported to be involved in the modification of the lipid metabolism in chickens fed with inulin. A nutrigenomic approach through the analysis of liver RNA hybridized to the Affymetrix GeneChip Chicken Genome Array identified 148 differentially expressed genes among both groups: 104 up-regulated (≥ 1.4-fold) and 44 down-regulated (≤ 0.6-fold). Quantitative real-time PCR analysis validated the microarray expression results for five out of seven genes tested. The functional annotation analyses revealed a number of genes, processes and pathways with putative involvement in chicken growth and performance, while reinforcing the immune status of animals, and fostering the production of long chain fatty acids in broilers supplemented with 5 g of inulin kg(-1) diet. As far as we are aware, this is the first report of a microarray based gene expression study on the effect of dietary inulin supplementation, supporting further research on the use of this prebiotic on chicken diets as a useful alternative to antibiotics for improving performance and general immunity in poultry farming, along with a healthier meat lipid profile.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0098942&type=printable |
| spellingShingle | Natalia Sevane Federica Bialade Susana Velasco Almudena Rebolé Maria Luisa Rodríguez Luís T Ortiz Javier Cañón Susana Dunner Dietary inulin supplementation modifies significantly the liver transcriptomic profile of broiler chickens. PLoS ONE |
| title | Dietary inulin supplementation modifies significantly the liver transcriptomic profile of broiler chickens. |
| title_full | Dietary inulin supplementation modifies significantly the liver transcriptomic profile of broiler chickens. |
| title_fullStr | Dietary inulin supplementation modifies significantly the liver transcriptomic profile of broiler chickens. |
| title_full_unstemmed | Dietary inulin supplementation modifies significantly the liver transcriptomic profile of broiler chickens. |
| title_short | Dietary inulin supplementation modifies significantly the liver transcriptomic profile of broiler chickens. |
| title_sort | dietary inulin supplementation modifies significantly the liver transcriptomic profile of broiler chickens |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0098942&type=printable |
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