Protective Effect of Ursodeoxycholic Acid in Experimental Endometriosis Induced Rat Model
Background: Considering the presence of an inflammatory process in the pathogenesis of endometriosis, anti-inflammatory agents could be an alternative option. The study aimed to elucidate the curative efficacy of Ursodeoxycholic acid (UDCA) on the experimental rat model of endometriosis. Methods: Th...
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IMR Press
2023-11-01
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| Series: | Clinical and Experimental Obstetrics & Gynecology |
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| Online Access: | https://www.imrpress.com/journal/CEOG/50/11/10.31083/j.ceog5011240 |
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| author | Ali Seven Suna Kabil Kucur Uğurkan Erkayiran Ayşe Nur Deger |
| author_facet | Ali Seven Suna Kabil Kucur Uğurkan Erkayiran Ayşe Nur Deger |
| author_sort | Ali Seven |
| collection | DOAJ |
| description | Background: Considering the presence of an inflammatory process in the pathogenesis of endometriosis, anti-inflammatory agents could be an alternative option. The study aimed to elucidate the curative efficacy of Ursodeoxycholic acid (UDCA) on the experimental rat model of endometriosis. Methods: This experimental research included a total of 60 mature female Wistar albino rats (250 ± 50 g) with no pregnancy. They were grouped as Standard (n: 20), Laparoscopic Pretreatment (n: 10), Laparoscopic Posttreatment (Sham) (n: 10), UDCA-Pretreatment (n: 10) and UDCA-Posttreatment (n: 10). Transforming growth factor β1 (TGF-β1), matrix metallo-proteinases-2 (MMP-2), Tissue inhibitor of metalloproteinase-1 (TIMP-1), Tumor necrosis factor-α (TNF-α) were analyzed. Results: In the UDCA post-treatment group, endometriotic focal volume (43.3 ± 24.04 mm3) was lower than the pre-treatment values (165.7 ± 21.7 mm3) (p = 0.005). There was no significant change UDCA group before and after the treatment in terms of MMP-2, TGF-β1, TIMP-1 and TNF-α levels (p > 0.05). Comparing the posttreatment values of the Sham srugery group and the UDCA group, while the endometriotic focal volume was 251 ± 51 mm3 in the Sham group, it decreased to 43.3 ± 24 mm3 in the UDCA (p < 0.0001). Histological scoring decreased from 2.6 ± 0.51 to 1 ± 0.81 after the treatment (p = 0.001). Conclusions: The pre-treatment laparotomy group exhibited elevated TNF-α levels, indicating an inflammatory response. UDCA treatment reduced endometriotic focal volume and histological scoring, indicating a potential therapeutic benefit. |
| format | Article |
| id | doaj-art-e89d309f1da64bc89e536db8329ad1a2 |
| institution | OA Journals |
| issn | 0390-6663 |
| language | English |
| publishDate | 2023-11-01 |
| publisher | IMR Press |
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| series | Clinical and Experimental Obstetrics & Gynecology |
| spelling | doaj-art-e89d309f1da64bc89e536db8329ad1a22025-08-20T02:22:15ZengIMR PressClinical and Experimental Obstetrics & Gynecology0390-66632023-11-01501124010.31083/j.ceog5011240S0390-6663(23)02081-XProtective Effect of Ursodeoxycholic Acid in Experimental Endometriosis Induced Rat ModelAli Seven0Suna Kabil Kucur1Uğurkan Erkayiran2Ayşe Nur Deger3Now with Department of Obstetrics and Gynecology, Cyprus Health and Social Sciences University Faculty of Medicine, 99700 Guzelyurt, North CyprusDepartment of Obstetrics and Gynecology, Marmara University Faculty of Medicine, 34000 Istanbul, TurkeyNow with Department of Obstetrics and Gynecology, Gaziantep Medical Point Hospital, 27050 Gaziantep, TurkeyDepartment of Pathology, Kütahya Health Sciences University, 43100 Kütahya, TurkeyBackground: Considering the presence of an inflammatory process in the pathogenesis of endometriosis, anti-inflammatory agents could be an alternative option. The study aimed to elucidate the curative efficacy of Ursodeoxycholic acid (UDCA) on the experimental rat model of endometriosis. Methods: This experimental research included a total of 60 mature female Wistar albino rats (250 ± 50 g) with no pregnancy. They were grouped as Standard (n: 20), Laparoscopic Pretreatment (n: 10), Laparoscopic Posttreatment (Sham) (n: 10), UDCA-Pretreatment (n: 10) and UDCA-Posttreatment (n: 10). Transforming growth factor β1 (TGF-β1), matrix metallo-proteinases-2 (MMP-2), Tissue inhibitor of metalloproteinase-1 (TIMP-1), Tumor necrosis factor-α (TNF-α) were analyzed. Results: In the UDCA post-treatment group, endometriotic focal volume (43.3 ± 24.04 mm3) was lower than the pre-treatment values (165.7 ± 21.7 mm3) (p = 0.005). There was no significant change UDCA group before and after the treatment in terms of MMP-2, TGF-β1, TIMP-1 and TNF-α levels (p > 0.05). Comparing the posttreatment values of the Sham srugery group and the UDCA group, while the endometriotic focal volume was 251 ± 51 mm3 in the Sham group, it decreased to 43.3 ± 24 mm3 in the UDCA (p < 0.0001). Histological scoring decreased from 2.6 ± 0.51 to 1 ± 0.81 after the treatment (p = 0.001). Conclusions: The pre-treatment laparotomy group exhibited elevated TNF-α levels, indicating an inflammatory response. UDCA treatment reduced endometriotic focal volume and histological scoring, indicating a potential therapeutic benefit.https://www.imrpress.com/journal/CEOG/50/11/10.31083/j.ceog5011240endometriosisursodeoxycholic acidmetalloproteinasetumor necrosis factor-α |
| spellingShingle | Ali Seven Suna Kabil Kucur Uğurkan Erkayiran Ayşe Nur Deger Protective Effect of Ursodeoxycholic Acid in Experimental Endometriosis Induced Rat Model Clinical and Experimental Obstetrics & Gynecology endometriosis ursodeoxycholic acid metalloproteinase tumor necrosis factor-α |
| title | Protective Effect of Ursodeoxycholic Acid in Experimental Endometriosis Induced Rat Model |
| title_full | Protective Effect of Ursodeoxycholic Acid in Experimental Endometriosis Induced Rat Model |
| title_fullStr | Protective Effect of Ursodeoxycholic Acid in Experimental Endometriosis Induced Rat Model |
| title_full_unstemmed | Protective Effect of Ursodeoxycholic Acid in Experimental Endometriosis Induced Rat Model |
| title_short | Protective Effect of Ursodeoxycholic Acid in Experimental Endometriosis Induced Rat Model |
| title_sort | protective effect of ursodeoxycholic acid in experimental endometriosis induced rat model |
| topic | endometriosis ursodeoxycholic acid metalloproteinase tumor necrosis factor-α |
| url | https://www.imrpress.com/journal/CEOG/50/11/10.31083/j.ceog5011240 |
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