Silver Nanoparticles from <i>Hermetia illucens</i> Biomass Are Antibacterial Against <i>Pseudomonas aeruginosa</i> Infection in <i>Caenorhabditis elegans</i>
Silver nanoparticles (AgNPs) are known for their antimicrobial properties, and can be synthesized through various routes. We used both chemical synthesis and green synthesis from the biomass of black soldier larvae (<i>Hermetia illucens</i>). To test the antimicrobial potential of these...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-05-01
|
| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/13/6/1277 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849425785587236864 |
|---|---|
| author | Alinne Ayech Gabriela Hollmann Robson M. Marreiro Gomes Belisa A. Rodrigues Vanessa K. Engers Rafael S. Gonçalves Sandro Daniel Nornberg Daniela F. Ramos Karine Rigon Zimmer José M. Monserrat |
| author_facet | Alinne Ayech Gabriela Hollmann Robson M. Marreiro Gomes Belisa A. Rodrigues Vanessa K. Engers Rafael S. Gonçalves Sandro Daniel Nornberg Daniela F. Ramos Karine Rigon Zimmer José M. Monserrat |
| author_sort | Alinne Ayech |
| collection | DOAJ |
| description | Silver nanoparticles (AgNPs) are known for their antimicrobial properties, and can be synthesized through various routes. We used both chemical synthesis and green synthesis from the biomass of black soldier larvae (<i>Hermetia illucens</i>). To test the antimicrobial potential of these nanoparticles, we employed an in vitro test, with CFU counting, and also used the worm <i>Caenorhabditis elegans</i> as an in vivo model. <i>C. elegans</i> were infected with <i>Pseudomonas aeruginosa</i> and treated with AgNPs from both syntheses. These AgNPs exhibited absorption spectrum peaks around 400 nm and sizes of 8 ± 3.5 (chemical) and 11 ± 4.7 nm (green). <i>P. aeruginosa</i>-infected worms, without treatment with AgNPs, achieved 100% mortality within 3 days, while AgNPs-treated worms survived until the end of the experiment, with no statistical differences compared to the non-infected worms of the control group. The results demonstrate that nanoparticles produced from <i>H. illucens</i> biomass have antimicrobial capacity, reducing bacterial growth in vitro and being able to protect <i>C. elegans</i> from infection by <i>P. aeruginosa</i>, similar to those produced by chemical synthesis. However, AgNPs from green synthesis are less harmful to the environment while maintaining their antimicrobial potential. |
| format | Article |
| id | doaj-art-e89bbd8b67bf48df9082720f435f7f78 |
| institution | Kabale University |
| issn | 2076-2607 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj-art-e89bbd8b67bf48df9082720f435f7f782025-08-20T03:29:39ZengMDPI AGMicroorganisms2076-26072025-05-01136127710.3390/microorganisms13061277Silver Nanoparticles from <i>Hermetia illucens</i> Biomass Are Antibacterial Against <i>Pseudomonas aeruginosa</i> Infection in <i>Caenorhabditis elegans</i>Alinne Ayech0Gabriela Hollmann1Robson M. Marreiro Gomes2Belisa A. Rodrigues3Vanessa K. Engers4Rafael S. Gonçalves5Sandro Daniel Nornberg6Daniela F. Ramos7Karine Rigon Zimmer8José M. Monserrat9Programa de Pós-Graduação em Ciências Fisiológicas (PPGCF), Instituto de Ciências Biológicas (ICB), Universidade Federal do Rio Grande-FURG, Rio Grande 96210-030, RS, BrazilPrograma de Pós-Graduação em Ciências Fisiológicas (PPGCF), Instituto de Ciências Biológicas (ICB), Universidade Federal do Rio Grande-FURG, Rio Grande 96210-030, RS, BrazilPrograma de Pós-Graduação em Aquicultura (PPGAq), Instituto de Oceanografia (IO), Universidade Federal do Rio Grande-FURG, Rio Grande 96200-970, RS, BrazilPrograma de Pós-Graduação em Biociências (PPGBio), Universidade Federal de Ciências da Saúde de Porto Alegre, Porto Alegre 90050-170, RS, BrazilPrograma de Pós-Graduação em Biociências (PPGBio), Universidade Federal de Ciências da Saúde de Porto Alegre, Porto Alegre 90050-170, RS, BrazilNui Insect Science Bioinsumos e Biotecnologia LTDA, Avenue Domingos José de Almeida, 1785, sala 4 i, Areal, Pelotas 96085-470, RS, BrazilNui Insect Science Bioinsumos e Biotecnologia LTDA, Avenue Domingos José de Almeida, 1785, sala 4 i, Areal, Pelotas 96085-470, RS, BrazilPrograma de Pós-Graduação em Ciências da Saúde (PPGCS), Faculdade de Medicina (FAMED), Universidade Federal do Rio Grande-FURG, Rio Grande 96200-190, RS, BrazilPrograma de Pós-Graduação em Biociências (PPGBio), Universidade Federal de Ciências da Saúde de Porto Alegre, Porto Alegre 90050-170, RS, BrazilPrograma de Pós-Graduação em Ciências Fisiológicas (PPGCF), Instituto de Ciências Biológicas (ICB), Universidade Federal do Rio Grande-FURG, Rio Grande 96210-030, RS, BrazilSilver nanoparticles (AgNPs) are known for their antimicrobial properties, and can be synthesized through various routes. We used both chemical synthesis and green synthesis from the biomass of black soldier larvae (<i>Hermetia illucens</i>). To test the antimicrobial potential of these nanoparticles, we employed an in vitro test, with CFU counting, and also used the worm <i>Caenorhabditis elegans</i> as an in vivo model. <i>C. elegans</i> were infected with <i>Pseudomonas aeruginosa</i> and treated with AgNPs from both syntheses. These AgNPs exhibited absorption spectrum peaks around 400 nm and sizes of 8 ± 3.5 (chemical) and 11 ± 4.7 nm (green). <i>P. aeruginosa</i>-infected worms, without treatment with AgNPs, achieved 100% mortality within 3 days, while AgNPs-treated worms survived until the end of the experiment, with no statistical differences compared to the non-infected worms of the control group. The results demonstrate that nanoparticles produced from <i>H. illucens</i> biomass have antimicrobial capacity, reducing bacterial growth in vitro and being able to protect <i>C. elegans</i> from infection by <i>P. aeruginosa</i>, similar to those produced by chemical synthesis. However, AgNPs from green synthesis are less harmful to the environment while maintaining their antimicrobial potential.https://www.mdpi.com/2076-2607/13/6/1277nanotechnologybacterial infection<i>P. aeruginosa</i>antimicrobial<i>C. elegans</i>green synthesis |
| spellingShingle | Alinne Ayech Gabriela Hollmann Robson M. Marreiro Gomes Belisa A. Rodrigues Vanessa K. Engers Rafael S. Gonçalves Sandro Daniel Nornberg Daniela F. Ramos Karine Rigon Zimmer José M. Monserrat Silver Nanoparticles from <i>Hermetia illucens</i> Biomass Are Antibacterial Against <i>Pseudomonas aeruginosa</i> Infection in <i>Caenorhabditis elegans</i> Microorganisms nanotechnology bacterial infection <i>P. aeruginosa</i> antimicrobial <i>C. elegans</i> green synthesis |
| title | Silver Nanoparticles from <i>Hermetia illucens</i> Biomass Are Antibacterial Against <i>Pseudomonas aeruginosa</i> Infection in <i>Caenorhabditis elegans</i> |
| title_full | Silver Nanoparticles from <i>Hermetia illucens</i> Biomass Are Antibacterial Against <i>Pseudomonas aeruginosa</i> Infection in <i>Caenorhabditis elegans</i> |
| title_fullStr | Silver Nanoparticles from <i>Hermetia illucens</i> Biomass Are Antibacterial Against <i>Pseudomonas aeruginosa</i> Infection in <i>Caenorhabditis elegans</i> |
| title_full_unstemmed | Silver Nanoparticles from <i>Hermetia illucens</i> Biomass Are Antibacterial Against <i>Pseudomonas aeruginosa</i> Infection in <i>Caenorhabditis elegans</i> |
| title_short | Silver Nanoparticles from <i>Hermetia illucens</i> Biomass Are Antibacterial Against <i>Pseudomonas aeruginosa</i> Infection in <i>Caenorhabditis elegans</i> |
| title_sort | silver nanoparticles from i hermetia illucens i biomass are antibacterial against i pseudomonas aeruginosa i infection in i caenorhabditis elegans i |
| topic | nanotechnology bacterial infection <i>P. aeruginosa</i> antimicrobial <i>C. elegans</i> green synthesis |
| url | https://www.mdpi.com/2076-2607/13/6/1277 |
| work_keys_str_mv | AT alinneayech silvernanoparticlesfromihermetiaillucensibiomassareantibacterialagainstipseudomonasaeruginosaiinfectioninicaenorhabditiselegansi AT gabrielahollmann silvernanoparticlesfromihermetiaillucensibiomassareantibacterialagainstipseudomonasaeruginosaiinfectioninicaenorhabditiselegansi AT robsonmmarreirogomes silvernanoparticlesfromihermetiaillucensibiomassareantibacterialagainstipseudomonasaeruginosaiinfectioninicaenorhabditiselegansi AT belisaarodrigues silvernanoparticlesfromihermetiaillucensibiomassareantibacterialagainstipseudomonasaeruginosaiinfectioninicaenorhabditiselegansi AT vanessakengers silvernanoparticlesfromihermetiaillucensibiomassareantibacterialagainstipseudomonasaeruginosaiinfectioninicaenorhabditiselegansi AT rafaelsgoncalves silvernanoparticlesfromihermetiaillucensibiomassareantibacterialagainstipseudomonasaeruginosaiinfectioninicaenorhabditiselegansi AT sandrodanielnornberg silvernanoparticlesfromihermetiaillucensibiomassareantibacterialagainstipseudomonasaeruginosaiinfectioninicaenorhabditiselegansi AT danielaframos silvernanoparticlesfromihermetiaillucensibiomassareantibacterialagainstipseudomonasaeruginosaiinfectioninicaenorhabditiselegansi AT karinerigonzimmer silvernanoparticlesfromihermetiaillucensibiomassareantibacterialagainstipseudomonasaeruginosaiinfectioninicaenorhabditiselegansi AT josemmonserrat silvernanoparticlesfromihermetiaillucensibiomassareantibacterialagainstipseudomonasaeruginosaiinfectioninicaenorhabditiselegansi |