Identification and functional analysis of six DNAJ genes from Myzus persicae (Hemiptera: Aphididae) in response to UV-B stress
Ultraviolet B (UV-B) is a significant environmental factor affecting insect development, survival, and reproduction. DNAJ proteins are molecular chaperones found ubiquitously in insects that are crucial for their adaptation to environmental stresses. This study aimed to elucidate the roles of DNAJ g...
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Institute of Entomology, Biology Centre, Czech Academy of Science
2024-07-01
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| Series: | European Journal of Entomology |
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| Online Access: | https://www.eje.cz/artkey/eje-202401-0027_identification-and-functional-analysis-of-six-dnaj-genes-from-myzus-persicae-hemiptera-aphididae-in-response.php |
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| author | Long-Chun HE Jian-Yu MENG Guy SMAGGHE Chang-Li YANG Xue TANG Chang-Yu ZHANG |
| author_facet | Long-Chun HE Jian-Yu MENG Guy SMAGGHE Chang-Li YANG Xue TANG Chang-Yu ZHANG |
| author_sort | Long-Chun HE |
| collection | DOAJ |
| description | Ultraviolet B (UV-B) is a significant environmental factor affecting insect development, survival, and reproduction. DNAJ proteins are molecular chaperones found ubiquitously in insects that are crucial for their adaptation to environmental stresses. This study aimed to elucidate the roles of DNAJ genes in the response of Myzus persicae to UV-B stress. Herein, we identified six DNAJ genes in the aphid M. persicae, a devastating agricultural pest. We analyzed their expression profiles at different stages of development, in different tissues and for various durations of UV-B exposure. The expression levels of MpDNAJC30, MpDNAJC11, MpDNAJC2, and MpDNAJC3 were highest in wingless adults, while MpDNAJC9 and MpDNAJC17 were highest in second- and third-instar nymphs, respectively. Six MpDNAJs had higher expression levels in the epidermis and embryos, and lower levels in the head. Additionally, the expression levels of all genes increased significantly under different durations of UV-B exposure. Knockout of the DNAJ genes using RNA interference caused a significant decline in the survival rate, weight, body length, and body width of M. persicae exposed to UV-B radiation. Our research provides valuable insights into the stress response mechanisms of M. persicae, highlighting the importance of DNAJ genes in mediating their adaptation to UV-B stress. |
| format | Article |
| id | doaj-art-e89adb7007864fb588e5fc856e8c1149 |
| institution | DOAJ |
| issn | 1210-5759 1802-8829 |
| language | English |
| publishDate | 2024-07-01 |
| publisher | Institute of Entomology, Biology Centre, Czech Academy of Science |
| record_format | Article |
| series | European Journal of Entomology |
| spelling | doaj-art-e89adb7007864fb588e5fc856e8c11492025-08-20T02:49:19ZengInstitute of Entomology, Biology Centre, Czech Academy of ScienceEuropean Journal of Entomology1210-57591802-88292024-07-01121126026810.14411/eje.2024.027eje-202401-0027Identification and functional analysis of six DNAJ genes from Myzus persicae (Hemiptera: Aphididae) in response to UV-B stressLong-Chun HE0Jian-Yu MENG1Guy SMAGGHE2Chang-Li YANG3Xue TANG4Chang-Yu ZHANG5Institute of Entomology, Guizhou Provincial Key Laboratory for Agricultural Pest Management of the Mountainous Region, Guizhou University, Guiyang, Guizhou 550025, China; e-mails: helongchun0303@gmail.com, guysma9@gmail.com, yangchangli@126.com, aneira1994@163.com, gzuzcy1121@126.comGuizhou Tobacco Science Research Institute, Guiyang, Guizhou 550081, China; e-mail: mengjy0417@163.comInstitute of Entomology, Guizhou Provincial Key Laboratory for Agricultural Pest Management of the Mountainous Region, Guizhou University, Guiyang, Guizhou 550025, China; e-mails: helongchun0303@gmail.com, guysma9@gmail.com, yangchangli@126.com, aneira1994@163.com, gzuzcy1121@126.comInstitute of Entomology, Guizhou Provincial Key Laboratory for Agricultural Pest Management of the Mountainous Region, Guizhou University, Guiyang, Guizhou 550025, China; e-mails: helongchun0303@gmail.com, guysma9@gmail.com, yangchangli@126.com, aneira1994@163.com, gzuzcy1121@126.comInstitute of Entomology, Guizhou Provincial Key Laboratory for Agricultural Pest Management of the Mountainous Region, Guizhou University, Guiyang, Guizhou 550025, China; e-mails: helongchun0303@gmail.com, guysma9@gmail.com, yangchangli@126.com, aneira1994@163.com, gzuzcy1121@126.comInstitute of Entomology, Guizhou Provincial Key Laboratory for Agricultural Pest Management of the Mountainous Region, Guizhou University, Guiyang, Guizhou 550025, China; e-mails: helongchun0303@gmail.com, guysma9@gmail.com, yangchangli@126.com, aneira1994@163.com, gzuzcy1121@126.comUltraviolet B (UV-B) is a significant environmental factor affecting insect development, survival, and reproduction. DNAJ proteins are molecular chaperones found ubiquitously in insects that are crucial for their adaptation to environmental stresses. This study aimed to elucidate the roles of DNAJ genes in the response of Myzus persicae to UV-B stress. Herein, we identified six DNAJ genes in the aphid M. persicae, a devastating agricultural pest. We analyzed their expression profiles at different stages of development, in different tissues and for various durations of UV-B exposure. The expression levels of MpDNAJC30, MpDNAJC11, MpDNAJC2, and MpDNAJC3 were highest in wingless adults, while MpDNAJC9 and MpDNAJC17 were highest in second- and third-instar nymphs, respectively. Six MpDNAJs had higher expression levels in the epidermis and embryos, and lower levels in the head. Additionally, the expression levels of all genes increased significantly under different durations of UV-B exposure. Knockout of the DNAJ genes using RNA interference caused a significant decline in the survival rate, weight, body length, and body width of M. persicae exposed to UV-B radiation. Our research provides valuable insights into the stress response mechanisms of M. persicae, highlighting the importance of DNAJ genes in mediating their adaptation to UV-B stress.https://www.eje.cz/artkey/eje-202401-0027_identification-and-functional-analysis-of-six-dnaj-genes-from-myzus-persicae-hemiptera-aphididae-in-response.phpaphidagricultural pestheat shock proteinsrnaiuv radiation stress |
| spellingShingle | Long-Chun HE Jian-Yu MENG Guy SMAGGHE Chang-Li YANG Xue TANG Chang-Yu ZHANG Identification and functional analysis of six DNAJ genes from Myzus persicae (Hemiptera: Aphididae) in response to UV-B stress European Journal of Entomology aphid agricultural pest heat shock proteins rnai uv radiation stress |
| title | Identification and functional analysis of six DNAJ genes from Myzus persicae (Hemiptera: Aphididae) in response to UV-B stress |
| title_full | Identification and functional analysis of six DNAJ genes from Myzus persicae (Hemiptera: Aphididae) in response to UV-B stress |
| title_fullStr | Identification and functional analysis of six DNAJ genes from Myzus persicae (Hemiptera: Aphididae) in response to UV-B stress |
| title_full_unstemmed | Identification and functional analysis of six DNAJ genes from Myzus persicae (Hemiptera: Aphididae) in response to UV-B stress |
| title_short | Identification and functional analysis of six DNAJ genes from Myzus persicae (Hemiptera: Aphididae) in response to UV-B stress |
| title_sort | identification and functional analysis of six dnaj genes from myzus persicae hemiptera aphididae in response to uv b stress |
| topic | aphid agricultural pest heat shock proteins rnai uv radiation stress |
| url | https://www.eje.cz/artkey/eje-202401-0027_identification-and-functional-analysis-of-six-dnaj-genes-from-myzus-persicae-hemiptera-aphididae-in-response.php |
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