A bHLH Transcription Factor Confers Salinity Stress Tolerance in Betula platyphylla
ABSTRACT Basic helix–loop–helix (bHLH) proteins comprise a large family of transcription factors that are involved in plant growth and development, as well as responses to various types of environmental stress. Betula platyphylla (birch) is a pioneer tree species in secondary forest that plays a key...
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| Format: | Article |
| Language: | English |
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Wiley
2024-12-01
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| Series: | Plant Direct |
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| Online Access: | https://doi.org/10.1002/pld3.70029 |
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| author | Qilong Fang Di Wu Hu Sun Luyao Wang Yuping Liu Wenfeng Mei Huiyan Guo |
| author_facet | Qilong Fang Di Wu Hu Sun Luyao Wang Yuping Liu Wenfeng Mei Huiyan Guo |
| author_sort | Qilong Fang |
| collection | DOAJ |
| description | ABSTRACT Basic helix–loop–helix (bHLH) proteins comprise a large family of transcription factors that are involved in plant growth and development, as well as responses to various types of environmental stress. Betula platyphylla (birch) is a pioneer tree species in secondary forest that plays a key role in maintaining ecosystem stability and forest regeneration, but few bHLHs involved in abiotic stress responses have been unveiled in birch. In this study, nine BpbHLH TFs related to stress responses in the birch genome were identified. Quantitative real‐time polymerase chain reaction (RT‐PCR) analysis indicated that the expression of these TFs can be induced by salt stress, and the expression of BpbHLH1 was higher than that of other BpbHLH genes. Particle bombardment analysis revealed that BpbHLH1 was localized to the nucleus. Yeast transformation found that BpbHLH1 has transcriptional activation activity. We generated BpbHLH1‐overexpressing and silencing transgenic birch plants and subjected them to salt stress analysis. BpbHLH1 can enhance the salt tolerance of birch plants by increasing the reactive oxygen species scavenging ability and inhibiting cell death. Yeast one‐hybrid, ß‐glucuronidase, and chromatin immunoprecipitation assays revealed that BpbHLH1 can regulate the expression of target genes involved in stress resistance by binding to the E‐box‐1, E‐box‐2 and G‐box elements in their promoters. The results of this study enhanced our understanding of the salt tolerance conferred by BpbHLH TFs in B. platyphylla and identified useful genes for the breeding of novel birch germplasm. |
| format | Article |
| id | doaj-art-e891b9690adf4c4d9736dc7203d1d46e |
| institution | Kabale University |
| issn | 2475-4455 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Wiley |
| record_format | Article |
| series | Plant Direct |
| spelling | doaj-art-e891b9690adf4c4d9736dc7203d1d46e2024-12-27T03:50:28ZengWileyPlant Direct2475-44552024-12-01812n/an/a10.1002/pld3.70029A bHLH Transcription Factor Confers Salinity Stress Tolerance in Betula platyphyllaQilong Fang0Di Wu1Hu Sun2Luyao Wang3Yuping Liu4Wenfeng Mei5Huiyan Guo6College of Forestry Shenyang Agricultural University Shenyang ChinaCollege of Forestry Shenyang Agricultural University Shenyang ChinaCollege of Forestry Shenyang Agricultural University Shenyang ChinaCollege of Forestry Shenyang Agricultural University Shenyang ChinaState Owned Forest Farm Management Service Center, Kuandian Manchu Autonomous County Dandong ChinaCollege of Forestry Shenyang Agricultural University Shenyang ChinaCollege of Forestry Shenyang Agricultural University Shenyang ChinaABSTRACT Basic helix–loop–helix (bHLH) proteins comprise a large family of transcription factors that are involved in plant growth and development, as well as responses to various types of environmental stress. Betula platyphylla (birch) is a pioneer tree species in secondary forest that plays a key role in maintaining ecosystem stability and forest regeneration, but few bHLHs involved in abiotic stress responses have been unveiled in birch. In this study, nine BpbHLH TFs related to stress responses in the birch genome were identified. Quantitative real‐time polymerase chain reaction (RT‐PCR) analysis indicated that the expression of these TFs can be induced by salt stress, and the expression of BpbHLH1 was higher than that of other BpbHLH genes. Particle bombardment analysis revealed that BpbHLH1 was localized to the nucleus. Yeast transformation found that BpbHLH1 has transcriptional activation activity. We generated BpbHLH1‐overexpressing and silencing transgenic birch plants and subjected them to salt stress analysis. BpbHLH1 can enhance the salt tolerance of birch plants by increasing the reactive oxygen species scavenging ability and inhibiting cell death. Yeast one‐hybrid, ß‐glucuronidase, and chromatin immunoprecipitation assays revealed that BpbHLH1 can regulate the expression of target genes involved in stress resistance by binding to the E‐box‐1, E‐box‐2 and G‐box elements in their promoters. The results of this study enhanced our understanding of the salt tolerance conferred by BpbHLH TFs in B. platyphylla and identified useful genes for the breeding of novel birch germplasm.https://doi.org/10.1002/pld3.70029Betula platyphyllabHLH transcription factorsalt stress tolerance |
| spellingShingle | Qilong Fang Di Wu Hu Sun Luyao Wang Yuping Liu Wenfeng Mei Huiyan Guo A bHLH Transcription Factor Confers Salinity Stress Tolerance in Betula platyphylla Plant Direct Betula platyphylla bHLH transcription factor salt stress tolerance |
| title | A bHLH Transcription Factor Confers Salinity Stress Tolerance in Betula platyphylla |
| title_full | A bHLH Transcription Factor Confers Salinity Stress Tolerance in Betula platyphylla |
| title_fullStr | A bHLH Transcription Factor Confers Salinity Stress Tolerance in Betula platyphylla |
| title_full_unstemmed | A bHLH Transcription Factor Confers Salinity Stress Tolerance in Betula platyphylla |
| title_short | A bHLH Transcription Factor Confers Salinity Stress Tolerance in Betula platyphylla |
| title_sort | bhlh transcription factor confers salinity stress tolerance in betula platyphylla |
| topic | Betula platyphylla bHLH transcription factor salt stress tolerance |
| url | https://doi.org/10.1002/pld3.70029 |
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