The protective role of adipogenic lineage precursors in maintaining bone marrow redox homeostasis in a mouse model of prenatal dexamethasone exposure
Bone marrow adipogenic precursors play important roles in bone metabolism in both young and adult mice, but their contributions to early long bone development remains poorly understood. In this study, we elucidate the role of bone marrow adipocyte lineage precursors in modulating bone marrow redox h...
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Elsevier
2025-10-01
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| Series: | Redox Biology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213231725003337 |
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| author | Jianwen Su Sushuang Ma Mankai Yang Jichang Wu Yijie Chen Mingchao Jin Qin Shi Xianrong Zhang |
| author_facet | Jianwen Su Sushuang Ma Mankai Yang Jichang Wu Yijie Chen Mingchao Jin Qin Shi Xianrong Zhang |
| author_sort | Jianwen Su |
| collection | DOAJ |
| description | Bone marrow adipogenic precursors play important roles in bone metabolism in both young and adult mice, but their contributions to early long bone development remains poorly understood. In this study, we elucidate the role of bone marrow adipocyte lineage precursors in modulating bone marrow redox homeostasis through the secretion of fibulin-5 (Fbln5), using a prenatal dexamethasone exposure (PDE) mouse model. Our previous research demonstrates that PDE induces cellular senescence in the bone marrow, resulting in long bone growth retardation in young offspring. Extending these findings, we now reveal that PDE not only induces cellular senescence and impairs bone formation, but also disrupts type H vessels and reduces Adiponectin-expressing (Adipoq+) cells. Importantly, genetic ablation of Adipoq+ cells recapitulates the phenotypes observed in PDE-exposed offspring, characterized by increased cellular senescence and loss of osteoblasts and osteoprogenitors during the early postnatal period, ultimately resulting in reduced trabecular bone mass in young adult mice. RNA-seq and in vivo data identify that Adipoq+ cells are a primary source of Fbln5, and that PDE significantly reduced the number of Adipoq+ cells, thereby decreasing Fbln5 expression and elevating ROS stress in bone marrow. Moreover, targeted overexpression of Fbln5 in Adipoq+ cells via adeno-associated virus effectively mitigates cellular senescence and ROS accumulation, preserves type H vessels and osteoblasts, and normalizes osteoclasts activity, thereby rescuing the long bone growth retardation caused by PDE. Collectively, these findings uncover a previously unrecognized function of Adipoq+ cells in regulating redox homeostasis within the bone marrow microenvironment during the early stages of long bone development. |
| format | Article |
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| institution | Kabale University |
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| language | English |
| publishDate | 2025-10-01 |
| publisher | Elsevier |
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| series | Redox Biology |
| spelling | doaj-art-e88dd80d24444da0a6dbb290859ae5492025-08-23T04:48:21ZengElsevierRedox Biology2213-23172025-10-018610382010.1016/j.redox.2025.103820The protective role of adipogenic lineage precursors in maintaining bone marrow redox homeostasis in a mouse model of prenatal dexamethasone exposureJianwen Su0Sushuang Ma1Mankai Yang2Jichang Wu3Yijie Chen4Mingchao Jin5Qin Shi6Xianrong Zhang7Division of Orthopaedics and Traumatology, Department of Orthopaedics, Nanfang Hospital, Southern Medical University, No.1838 North of Guangzhou Avenue, Guangzhou, 510515, Guangdong, China; Guangdong Provincial Key Laboratory of Bone and Cartilage Regenerative Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, Guangdong, ChinaDivision of Orthopaedics and Traumatology, Department of Orthopaedics, Nanfang Hospital, Southern Medical University, No.1838 North of Guangzhou Avenue, Guangzhou, 510515, Guangdong, China; Guangdong Provincial Key Laboratory of Bone and Cartilage Regenerative Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, Guangdong, ChinaDivision of Orthopaedics and Traumatology, Department of Orthopaedics, Nanfang Hospital, Southern Medical University, No.1838 North of Guangzhou Avenue, Guangzhou, 510515, Guangdong, China; Guangdong Provincial Key Laboratory of Bone and Cartilage Regenerative Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, Guangdong, ChinaDivision of Orthopaedics and Traumatology, Department of Orthopaedics, Nanfang Hospital, Southern Medical University, No.1838 North of Guangzhou Avenue, Guangzhou, 510515, Guangdong, China; Guangdong Provincial Key Laboratory of Bone and Cartilage Regenerative Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, Guangdong, ChinaDivision of Orthopaedics and Traumatology, Department of Orthopaedics, Nanfang Hospital, Southern Medical University, No.1838 North of Guangzhou Avenue, Guangzhou, 510515, Guangdong, China; Guangdong Provincial Key Laboratory of Bone and Cartilage Regenerative Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, Guangdong, ChinaDivision of Orthopaedics and Traumatology, Department of Orthopaedics, Nanfang Hospital, Southern Medical University, No.1838 North of Guangzhou Avenue, Guangzhou, 510515, Guangdong, China; Guangdong Provincial Key Laboratory of Bone and Cartilage Regenerative Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, Guangdong, ChinaDepartment of Orthopedics, The First Affiliated Hospital of Soochow University, Medical College of Soochow University, Orthopedic Institute of Soochow University, 899 Pinghai Road, Suzhou, Jiangsu, 215031, ChinaDivision of Orthopaedics and Traumatology, Department of Orthopaedics, Nanfang Hospital, Southern Medical University, No.1838 North of Guangzhou Avenue, Guangzhou, 510515, Guangdong, China; Guangdong Provincial Key Laboratory of Bone and Cartilage Regenerative Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, Guangdong, China; Corresponding author. Division of Orthopaedics and Traumatology, Department of Orthopaedics, Nanfang Hospital, Southern Medical University, No.1838 North of Guangzhou Avenue, Guangzhou, Guangdong Province, 510515, China.Bone marrow adipogenic precursors play important roles in bone metabolism in both young and adult mice, but their contributions to early long bone development remains poorly understood. In this study, we elucidate the role of bone marrow adipocyte lineage precursors in modulating bone marrow redox homeostasis through the secretion of fibulin-5 (Fbln5), using a prenatal dexamethasone exposure (PDE) mouse model. Our previous research demonstrates that PDE induces cellular senescence in the bone marrow, resulting in long bone growth retardation in young offspring. Extending these findings, we now reveal that PDE not only induces cellular senescence and impairs bone formation, but also disrupts type H vessels and reduces Adiponectin-expressing (Adipoq+) cells. Importantly, genetic ablation of Adipoq+ cells recapitulates the phenotypes observed in PDE-exposed offspring, characterized by increased cellular senescence and loss of osteoblasts and osteoprogenitors during the early postnatal period, ultimately resulting in reduced trabecular bone mass in young adult mice. RNA-seq and in vivo data identify that Adipoq+ cells are a primary source of Fbln5, and that PDE significantly reduced the number of Adipoq+ cells, thereby decreasing Fbln5 expression and elevating ROS stress in bone marrow. Moreover, targeted overexpression of Fbln5 in Adipoq+ cells via adeno-associated virus effectively mitigates cellular senescence and ROS accumulation, preserves type H vessels and osteoblasts, and normalizes osteoclasts activity, thereby rescuing the long bone growth retardation caused by PDE. Collectively, these findings uncover a previously unrecognized function of Adipoq+ cells in regulating redox homeostasis within the bone marrow microenvironment during the early stages of long bone development.http://www.sciencedirect.com/science/article/pii/S2213231725003337Adipogenic lineage precursorsFibulin 5Cellular senescenceRedox homeostasisPrenatal dexamethasone exposure |
| spellingShingle | Jianwen Su Sushuang Ma Mankai Yang Jichang Wu Yijie Chen Mingchao Jin Qin Shi Xianrong Zhang The protective role of adipogenic lineage precursors in maintaining bone marrow redox homeostasis in a mouse model of prenatal dexamethasone exposure Redox Biology Adipogenic lineage precursors Fibulin 5 Cellular senescence Redox homeostasis Prenatal dexamethasone exposure |
| title | The protective role of adipogenic lineage precursors in maintaining bone marrow redox homeostasis in a mouse model of prenatal dexamethasone exposure |
| title_full | The protective role of adipogenic lineage precursors in maintaining bone marrow redox homeostasis in a mouse model of prenatal dexamethasone exposure |
| title_fullStr | The protective role of adipogenic lineage precursors in maintaining bone marrow redox homeostasis in a mouse model of prenatal dexamethasone exposure |
| title_full_unstemmed | The protective role of adipogenic lineage precursors in maintaining bone marrow redox homeostasis in a mouse model of prenatal dexamethasone exposure |
| title_short | The protective role of adipogenic lineage precursors in maintaining bone marrow redox homeostasis in a mouse model of prenatal dexamethasone exposure |
| title_sort | protective role of adipogenic lineage precursors in maintaining bone marrow redox homeostasis in a mouse model of prenatal dexamethasone exposure |
| topic | Adipogenic lineage precursors Fibulin 5 Cellular senescence Redox homeostasis Prenatal dexamethasone exposure |
| url | http://www.sciencedirect.com/science/article/pii/S2213231725003337 |
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