Production and characterization of monoclonal antibody to duck IL-2
Five hybridoma cell lines secreting monoclonal antibody (mAb) against duck IL-2 were produced by fusing mouse myeloma cells (SP2/0) with spleen cells from BALB/c immunized with the purified recombinant duck IL-2 protein and named as 1H4, 2B3, 4G12, 5F6 and 5H6. One of the five hybridoma cell lines (...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2004-03-01
|
| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2004.02.0169 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850044834333589504 |
|---|---|
| author | GONG Hui WU Jian-xiang WANG Jin-yong CHEN Ji-gang ZHOU Ji-yong |
| author_facet | GONG Hui WU Jian-xiang WANG Jin-yong CHEN Ji-gang ZHOU Ji-yong |
| author_sort | GONG Hui |
| collection | DOAJ |
| description | Five hybridoma cell lines secreting monoclonal antibody (mAb) against duck IL-2 were produced by fusing mouse myeloma cells (SP2/0) with spleen cells from BALB/c immunized with the purified recombinant duck IL-2 protein and named as 1H4, 2B3, 4G12, 5F6 and 5H6. One of the five hybridoma cell lines (5H6) could also react with chicken IL-2. The titres of ascitic fluids of the five mAbs ranged from 1∶32000 to 1∶512000 by indirect ELISA. Among the five mAbs, 5H6 belongs to IgG2a, and the other mAbs were of the same isotype of murine antibodies IgG1, according to the Mouse Immunoglobulin Standard Panel. The five murine antibodies' light chain hold the same isotype, κ. Western-blot analysis showed that the five mAbs could react with duck IL-2, they are supposed to against continuous determinants of duck IL-2. |
| format | Article |
| id | doaj-art-e886a3498dcb42b58501e7f7dc83014d |
| institution | DOAJ |
| issn | 1008-9209 2097-5155 |
| language | English |
| publishDate | 2004-03-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | 浙江大学学报. 农业与生命科学版 |
| spelling | doaj-art-e886a3498dcb42b58501e7f7dc83014d2025-08-20T02:54:50ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552004-03-013016917210.3785/1008-9209.2004.02.016910089209Production and characterization of monoclonal antibody to duck IL-2GONG HuiWU Jian-xiangWANG Jin-yongCHEN Ji-gangZHOU Ji-yongFive hybridoma cell lines secreting monoclonal antibody (mAb) against duck IL-2 were produced by fusing mouse myeloma cells (SP2/0) with spleen cells from BALB/c immunized with the purified recombinant duck IL-2 protein and named as 1H4, 2B3, 4G12, 5F6 and 5H6. One of the five hybridoma cell lines (5H6) could also react with chicken IL-2. The titres of ascitic fluids of the five mAbs ranged from 1∶32000 to 1∶512000 by indirect ELISA. Among the five mAbs, 5H6 belongs to IgG2a, and the other mAbs were of the same isotype of murine antibodies IgG1, according to the Mouse Immunoglobulin Standard Panel. The five murine antibodies' light chain hold the same isotype, κ. Western-blot analysis showed that the five mAbs could react with duck IL-2, they are supposed to against continuous determinants of duck IL-2.https://www.academax.com/doi/10.3785/1008-9209.2004.02.0169duckIL-2monoclonal antibody |
| spellingShingle | GONG Hui WU Jian-xiang WANG Jin-yong CHEN Ji-gang ZHOU Ji-yong Production and characterization of monoclonal antibody to duck IL-2 浙江大学学报. 农业与生命科学版 duck IL-2 monoclonal antibody |
| title | Production and characterization of monoclonal antibody to duck IL-2 |
| title_full | Production and characterization of monoclonal antibody to duck IL-2 |
| title_fullStr | Production and characterization of monoclonal antibody to duck IL-2 |
| title_full_unstemmed | Production and characterization of monoclonal antibody to duck IL-2 |
| title_short | Production and characterization of monoclonal antibody to duck IL-2 |
| title_sort | production and characterization of monoclonal antibody to duck il 2 |
| topic | duck IL-2 monoclonal antibody |
| url | https://www.academax.com/doi/10.3785/1008-9209.2004.02.0169 |
| work_keys_str_mv | AT gonghui productionandcharacterizationofmonoclonalantibodytoduckil2 AT wujianxiang productionandcharacterizationofmonoclonalantibodytoduckil2 AT wangjinyong productionandcharacterizationofmonoclonalantibodytoduckil2 AT chenjigang productionandcharacterizationofmonoclonalantibodytoduckil2 AT zhoujiyong productionandcharacterizationofmonoclonalantibodytoduckil2 |