Effects of bile acids on the growth, composition and metabolism of gut bacteria
Abstract Bile acids (BAs) exert a profound influence on the body’s pathophysiology by intricately shaping the composition of gut bacteria. However, the complex interplay between BAs and gut microbiota has impeded a systematic exploration of their impact on intestinal bacteria. Initially, we investig...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-10-01
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| Series: | npj Biofilms and Microbiomes |
| Online Access: | https://doi.org/10.1038/s41522-024-00566-w |
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| author | Yi-Lei Peng Si-Han Wang Yu-Long Zhang Man-Yun Chen Kang He Qing Li Wei-Hua Huang Wei Zhang |
| author_facet | Yi-Lei Peng Si-Han Wang Yu-Long Zhang Man-Yun Chen Kang He Qing Li Wei-Hua Huang Wei Zhang |
| author_sort | Yi-Lei Peng |
| collection | DOAJ |
| description | Abstract Bile acids (BAs) exert a profound influence on the body’s pathophysiology by intricately shaping the composition of gut bacteria. However, the complex interplay between BAs and gut microbiota has impeded a systematic exploration of their impact on intestinal bacteria. Initially, we investigated the effects of 21 BAs on the growth of 65 gut bacterial strains in vitro. Subsequently, we examined the impact of BAs on the overall composition of intestinal bacteria, both in vivo and in vitro. The results unveiled distinct effects of various BAs on different intestinal strains and their diverse impacts on the composition of gut bacteria. Mechanistically, the inhibition of intestinal strains by BAs occurs through the accumulation of these acids within the strains. The intracellular accumulation of deoxycholic acid (DCA) significantly influenced the growth of intestinal bacteria by impacting ribosome transcription and amino-acid metabolism. The metabolomic analysis underscores the pronounced impact of DCA on amino-acid profiles in both in vivo and in vitro settings. This study not only elucidates the effects of BAs on a diverse range of bacterial strains and their role in shaping the gut microbiota but also reveals underlying mechanisms essential for understanding and maintaining a healthy gut microbiota. |
| format | Article |
| id | doaj-art-e8861121060e4269a3ec281bf34f3464 |
| institution | OA Journals |
| issn | 2055-5008 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | npj Biofilms and Microbiomes |
| spelling | doaj-art-e8861121060e4269a3ec281bf34f34642025-08-20T02:11:24ZengNature Portfolionpj Biofilms and Microbiomes2055-50082024-10-0110111310.1038/s41522-024-00566-wEffects of bile acids on the growth, composition and metabolism of gut bacteriaYi-Lei Peng0Si-Han Wang1Yu-Long Zhang2Man-Yun Chen3Kang He4Qing Li5Wei-Hua Huang6Wei Zhang7Department of Clinical Pharmacology, Xiangya Hospital, Central South UniversityDepartment of Clinical Pharmacology, Xiangya Hospital, Central South UniversityDepartment of Clinical Pharmacology, Xiangya Hospital, Central South UniversityDepartment of Clinical Pharmacology, Xiangya Hospital, Central South UniversityDepartment of Clinical Pharmacology, Xiangya Hospital, Central South UniversityDepartment of Clinical Pharmacology, Xiangya Hospital, Central South UniversityDepartment of Clinical Pharmacology, Xiangya Hospital, Central South UniversityDepartment of Clinical Pharmacology, Xiangya Hospital, Central South UniversityAbstract Bile acids (BAs) exert a profound influence on the body’s pathophysiology by intricately shaping the composition of gut bacteria. However, the complex interplay between BAs and gut microbiota has impeded a systematic exploration of their impact on intestinal bacteria. Initially, we investigated the effects of 21 BAs on the growth of 65 gut bacterial strains in vitro. Subsequently, we examined the impact of BAs on the overall composition of intestinal bacteria, both in vivo and in vitro. The results unveiled distinct effects of various BAs on different intestinal strains and their diverse impacts on the composition of gut bacteria. Mechanistically, the inhibition of intestinal strains by BAs occurs through the accumulation of these acids within the strains. The intracellular accumulation of deoxycholic acid (DCA) significantly influenced the growth of intestinal bacteria by impacting ribosome transcription and amino-acid metabolism. The metabolomic analysis underscores the pronounced impact of DCA on amino-acid profiles in both in vivo and in vitro settings. This study not only elucidates the effects of BAs on a diverse range of bacterial strains and their role in shaping the gut microbiota but also reveals underlying mechanisms essential for understanding and maintaining a healthy gut microbiota.https://doi.org/10.1038/s41522-024-00566-w |
| spellingShingle | Yi-Lei Peng Si-Han Wang Yu-Long Zhang Man-Yun Chen Kang He Qing Li Wei-Hua Huang Wei Zhang Effects of bile acids on the growth, composition and metabolism of gut bacteria npj Biofilms and Microbiomes |
| title | Effects of bile acids on the growth, composition and metabolism of gut bacteria |
| title_full | Effects of bile acids on the growth, composition and metabolism of gut bacteria |
| title_fullStr | Effects of bile acids on the growth, composition and metabolism of gut bacteria |
| title_full_unstemmed | Effects of bile acids on the growth, composition and metabolism of gut bacteria |
| title_short | Effects of bile acids on the growth, composition and metabolism of gut bacteria |
| title_sort | effects of bile acids on the growth composition and metabolism of gut bacteria |
| url | https://doi.org/10.1038/s41522-024-00566-w |
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