The Roles of Porous Coral Sands in Initial Enrichment of Ammonia-Oxidizing Bacteria
The purpose of this study was to investigate the roles of coral sands in the enrichment and isolation of ammonium-oxidizing bacteria (AOB). We hypothesized that the porous coral sands provided additional surface area and nutrients for the growth of periphytic AOB. In the present study, an orthogonal...
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| Format: | Article |
| Language: | English |
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Wiley
2007-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/tsw.2007.108 |
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| author | Qing Guo Zao-he Wu Ming-liang Qian Binhe Gu |
| author_facet | Qing Guo Zao-he Wu Ming-liang Qian Binhe Gu |
| author_sort | Qing Guo |
| collection | DOAJ |
| description | The purpose of this study was to investigate the roles of coral sands in the enrichment and isolation of ammonium-oxidizing bacteria (AOB). We hypothesized that the porous coral sands provided additional surface area and nutrients for the growth of periphytic AOB. In the present study, an orthogonal test was designed to compare the AOB conversion rates of ammonium-nitrogen (NH4+N) to nitrite-nitrogen (NO2--N) among various combinations of culture media. Results showed that the conversion of NH4+N to NO2--N increased significantly when the coral sands were added, implying that coral sands were beneficial to the growth of AOB. Additions of potassium dihydrogen phosphate (KH2PO4) or sodium bicarbonate (NaHCO3) to the media became unnecessary when coral sands were used, but the addition of KH2PO4 was needed when the molar nitrogen to phosphorus (N:P) ratio reached 10 in the enrichment media using calcium carbonate (CaCO3) powder as a calcium source. |
| format | Article |
| id | doaj-art-e88364ccbf12433abb5f84c0e9987cd8 |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2007-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-e88364ccbf12433abb5f84c0e9987cd82025-02-03T06:14:20ZengWileyThe Scientific World Journal1537-744X2007-01-01752553210.1100/tsw.2007.108The Roles of Porous Coral Sands in Initial Enrichment of Ammonia-Oxidizing BacteriaQing Guo0Zao-he Wu1Ming-liang Qian2Binhe Gu3South China Sea Institute of Oceanography, Chinese Academy of Sciences, 164 Xingangxi Road, Haizhu District, Guangzhou 510301, ChinaKey Laboratory of Pathogen Biology and Epidemiology of Aquatic Economic Animals of Guangdong Province, Guangdong Ocean University, 40 East Jiefang Road, Xiashan District, Zhanjiang 524025, ChinaKey Laboratory of Pathogen Biology and Epidemiology of Aquatic Economic Animals of Guangdong Province, Guangdong Ocean University, 40 East Jiefang Road, Xiashan District, Zhanjiang 524025, ChinaFishery College, Guangdong Ocean University, 40 East Jiefang Road, Xiashan District, Zhanjiang 524025, ChinaThe purpose of this study was to investigate the roles of coral sands in the enrichment and isolation of ammonium-oxidizing bacteria (AOB). We hypothesized that the porous coral sands provided additional surface area and nutrients for the growth of periphytic AOB. In the present study, an orthogonal test was designed to compare the AOB conversion rates of ammonium-nitrogen (NH4+N) to nitrite-nitrogen (NO2--N) among various combinations of culture media. Results showed that the conversion of NH4+N to NO2--N increased significantly when the coral sands were added, implying that coral sands were beneficial to the growth of AOB. Additions of potassium dihydrogen phosphate (KH2PO4) or sodium bicarbonate (NaHCO3) to the media became unnecessary when coral sands were used, but the addition of KH2PO4 was needed when the molar nitrogen to phosphorus (N:P) ratio reached 10 in the enrichment media using calcium carbonate (CaCO3) powder as a calcium source.http://dx.doi.org/10.1100/tsw.2007.108 |
| spellingShingle | Qing Guo Zao-he Wu Ming-liang Qian Binhe Gu The Roles of Porous Coral Sands in Initial Enrichment of Ammonia-Oxidizing Bacteria The Scientific World Journal |
| title | The Roles of Porous Coral Sands in Initial Enrichment of Ammonia-Oxidizing Bacteria |
| title_full | The Roles of Porous Coral Sands in Initial Enrichment of Ammonia-Oxidizing Bacteria |
| title_fullStr | The Roles of Porous Coral Sands in Initial Enrichment of Ammonia-Oxidizing Bacteria |
| title_full_unstemmed | The Roles of Porous Coral Sands in Initial Enrichment of Ammonia-Oxidizing Bacteria |
| title_short | The Roles of Porous Coral Sands in Initial Enrichment of Ammonia-Oxidizing Bacteria |
| title_sort | roles of porous coral sands in initial enrichment of ammonia oxidizing bacteria |
| url | http://dx.doi.org/10.1100/tsw.2007.108 |
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