Quantification of Sunscreen Ethylhexyl Triazone in Topical Skin-Care Products by Normal-Phase TLC/Densitometry
Ethylhexyl triazone (ET) was separated from other sunscreens such as avobenzone, octocrylene, octyl methoxycinnamate, and diethylamino hydroxybenzoyl hexyl benzoate and from parabens by normal-phase HPTLC on silica gel 60 as stationary phase. Two mobile phases were particularly effective: (A) cycloh...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/2012/807516 |
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| author | Anna W. Sobanska Jaroslaw Pyzowski |
| author_facet | Anna W. Sobanska Jaroslaw Pyzowski |
| author_sort | Anna W. Sobanska |
| collection | DOAJ |
| description | Ethylhexyl triazone (ET) was separated from other sunscreens such as avobenzone, octocrylene, octyl methoxycinnamate, and diethylamino hydroxybenzoyl hexyl benzoate and from parabens by normal-phase HPTLC on silica gel 60 as stationary phase. Two mobile phases were particularly effective: (A) cyclohexane-diethyl ether 1 : 1 (v/v) and (B) cyclohexane-diethyl ether-acetone 15 : 1 : 2 (v/v/v) since apart from ET analysis they facilitated separation and quantification of other sunscreens present in the formulations. Densitometric scanning was performed at 300 nm. Calibration curves for ET were nonlinear (second-degree polynomials), with R > 0.998. For both mobile phases limits of detection (LOD) were 0.03 and limits of quantification (LOQ) 0.1 μg spot−1. Both methods were validated. |
| format | Article |
| id | doaj-art-e88078203d6d4a5693395d9bafadc52a |
| institution | DOAJ |
| issn | 1537-744X |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-e88078203d6d4a5693395d9bafadc52a2025-08-20T03:21:02ZengWileyThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/807516807516Quantification of Sunscreen Ethylhexyl Triazone in Topical Skin-Care Products by Normal-Phase TLC/DensitometryAnna W. Sobanska0Jaroslaw Pyzowski1Department of Analytical Chemistry, Medical University of Lodz, Ulica Muszynskiego 1, 90-151 Lodz, PolandDepartment of Analytical Chemistry, Medical University of Lodz, Ulica Muszynskiego 1, 90-151 Lodz, PolandEthylhexyl triazone (ET) was separated from other sunscreens such as avobenzone, octocrylene, octyl methoxycinnamate, and diethylamino hydroxybenzoyl hexyl benzoate and from parabens by normal-phase HPTLC on silica gel 60 as stationary phase. Two mobile phases were particularly effective: (A) cyclohexane-diethyl ether 1 : 1 (v/v) and (B) cyclohexane-diethyl ether-acetone 15 : 1 : 2 (v/v/v) since apart from ET analysis they facilitated separation and quantification of other sunscreens present in the formulations. Densitometric scanning was performed at 300 nm. Calibration curves for ET were nonlinear (second-degree polynomials), with R > 0.998. For both mobile phases limits of detection (LOD) were 0.03 and limits of quantification (LOQ) 0.1 μg spot−1. Both methods were validated.http://dx.doi.org/10.1100/2012/807516 |
| spellingShingle | Anna W. Sobanska Jaroslaw Pyzowski Quantification of Sunscreen Ethylhexyl Triazone in Topical Skin-Care Products by Normal-Phase TLC/Densitometry The Scientific World Journal |
| title | Quantification of Sunscreen Ethylhexyl Triazone in Topical Skin-Care Products by Normal-Phase TLC/Densitometry |
| title_full | Quantification of Sunscreen Ethylhexyl Triazone in Topical Skin-Care Products by Normal-Phase TLC/Densitometry |
| title_fullStr | Quantification of Sunscreen Ethylhexyl Triazone in Topical Skin-Care Products by Normal-Phase TLC/Densitometry |
| title_full_unstemmed | Quantification of Sunscreen Ethylhexyl Triazone in Topical Skin-Care Products by Normal-Phase TLC/Densitometry |
| title_short | Quantification of Sunscreen Ethylhexyl Triazone in Topical Skin-Care Products by Normal-Phase TLC/Densitometry |
| title_sort | quantification of sunscreen ethylhexyl triazone in topical skin care products by normal phase tlc densitometry |
| url | http://dx.doi.org/10.1100/2012/807516 |
| work_keys_str_mv | AT annawsobanska quantificationofsunscreenethylhexyltriazoneintopicalskincareproductsbynormalphasetlcdensitometry AT jaroslawpyzowski quantificationofsunscreenethylhexyltriazoneintopicalskincareproductsbynormalphasetlcdensitometry |