Long-read sequencing and genome assembly of natural history collection samples and challenging specimens

Long-read sequencing and genome assembly of natural history collection samples and challenging specimens

Abstract Museum collections harbor millions of samples, largely unutilized for long-read sequencing. Here, we use ethanol-preserved samples containing kilobase-sized DNA to show that amplification-free protocols can yield contiguous genome assemblies. Additionally, using a modified amplification-bas...

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Bibliographic Details
Main Authors: Bernhard Bein, Ioannis Chrysostomakis, Larissa S. Arantes, Tom Brown, Charlotte Gerheim, Tilman Schell, Clément Schneider, Evgeny Leushkin, Zeyuan Chen, Julia Sigwart, Vanessa Gonzalez, Nur Leena W. S. Wong, Fabricio R. Santos, Mozes P. K. Blom, Frieder Mayer, Camila J. Mazzoni, Astrid Böhne, Sylke Winkler, Carola Greve, Michael Hiller
Format: Article
Language:English
Published: BMC 2025-02-01
Series:Genome Biology
Subjects:
Long-read sequencing
PCR amplification
Genome assembly
Museum collections
Online Access:https://doi.org/10.1186/s13059-025-03487-9
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