Determination of Raman spectrum under different culture conditions: preliminary research on bacterial fish pathogens
The intensive labour and time required for conventional methods to identify bacterial fish pathogens have revealed the need to develop alternative methods. Raman spectroscopy has been used in the rapid optical identification of bacterial pathogens in recent years as an alternative method in microbio...
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| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | Animal Biotechnology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/10495398.2023.2299733 |
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| author | Ezgi Dinçtürk |
| author_facet | Ezgi Dinçtürk |
| author_sort | Ezgi Dinçtürk |
| collection | DOAJ |
| description | The intensive labour and time required for conventional methods to identify bacterial fish pathogens have revealed the need to develop alternative methods. Raman spectroscopy has been used in the rapid optical identification of bacterial pathogens in recent years as an alternative method in microbiology. Strains of bacterial fish pathogens (Vibrio anguillarum, Lactococcus garvieae and Yersinia ruckeri) that often cause infectious diseases in fish were here identified and analyzed in terms of their biochemical structures in different media and at different incubation times, and the data were specified by using Raman spectroscopy. The results demonstrated that Raman spectroscopy presents species-specific Raman spectra of each disease-causing bacteria and that it would be more appropriate to choose general microbiological media over selective media for routine studies. Additionally, it was found that species-specific band regions did not differ in 24- and 48-hour cultures, but there could be a difference in peak intensity which may lead to difficult characterization of spectrum. The current study, conducted for the first time with bacterial fish pathogens under different incubation conditions, is believed to provide a basis for the routine use of Raman spectroscopy for quick pathogen identification and the precise determination of the methodology for further research. |
| format | Article |
| id | doaj-art-e8687d085b7d4562aea878750985d274 |
| institution | DOAJ |
| issn | 1049-5398 1532-2378 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Animal Biotechnology |
| spelling | doaj-art-e8687d085b7d4562aea878750985d2742025-08-20T02:46:58ZengTaylor & Francis GroupAnimal Biotechnology1049-53981532-23782024-12-0135110.1080/10495398.2023.2299733Determination of Raman spectrum under different culture conditions: preliminary research on bacterial fish pathogensEzgi Dinçtürk0Fish Disease and Biotechnology Laboratory, Department of Aquaculture, Izmir Katip Celebi University, Izmir, TürkiyeThe intensive labour and time required for conventional methods to identify bacterial fish pathogens have revealed the need to develop alternative methods. Raman spectroscopy has been used in the rapid optical identification of bacterial pathogens in recent years as an alternative method in microbiology. Strains of bacterial fish pathogens (Vibrio anguillarum, Lactococcus garvieae and Yersinia ruckeri) that often cause infectious diseases in fish were here identified and analyzed in terms of their biochemical structures in different media and at different incubation times, and the data were specified by using Raman spectroscopy. The results demonstrated that Raman spectroscopy presents species-specific Raman spectra of each disease-causing bacteria and that it would be more appropriate to choose general microbiological media over selective media for routine studies. Additionally, it was found that species-specific band regions did not differ in 24- and 48-hour cultures, but there could be a difference in peak intensity which may lead to difficult characterization of spectrum. The current study, conducted for the first time with bacterial fish pathogens under different incubation conditions, is believed to provide a basis for the routine use of Raman spectroscopy for quick pathogen identification and the precise determination of the methodology for further research.https://www.tandfonline.com/doi/10.1080/10495398.2023.2299733Raman spectroscopybacterial fish diseasesidentificationfish pathogens |
| spellingShingle | Ezgi Dinçtürk Determination of Raman spectrum under different culture conditions: preliminary research on bacterial fish pathogens Animal Biotechnology Raman spectroscopy bacterial fish diseases identification fish pathogens |
| title | Determination of Raman spectrum under different culture conditions: preliminary research on bacterial fish pathogens |
| title_full | Determination of Raman spectrum under different culture conditions: preliminary research on bacterial fish pathogens |
| title_fullStr | Determination of Raman spectrum under different culture conditions: preliminary research on bacterial fish pathogens |
| title_full_unstemmed | Determination of Raman spectrum under different culture conditions: preliminary research on bacterial fish pathogens |
| title_short | Determination of Raman spectrum under different culture conditions: preliminary research on bacterial fish pathogens |
| title_sort | determination of raman spectrum under different culture conditions preliminary research on bacterial fish pathogens |
| topic | Raman spectroscopy bacterial fish diseases identification fish pathogens |
| url | https://www.tandfonline.com/doi/10.1080/10495398.2023.2299733 |
| work_keys_str_mv | AT ezgidincturk determinationoframanspectrumunderdifferentcultureconditionspreliminaryresearchonbacterialfishpathogens |