Seroprevalence, Identification, and Pathology of Salmonellosis in Selected Poultry Farms at Barishal District of Bangladesh

Salmonellosis is a critical challenge in commercial poultry farming. This study aimed to calculate seroprevalence, identify Salmonella spp., and its pathological investigation from January to December 2019. One hundred fifty (150) serum samples and fifty (50) cloacal swabs from apparently ill and he...

Full description

Saved in:
Bibliographic Details
Main Authors: Shampa Rani, Khondoker Jahengir Alam, Shib Shankar Saha, Md. Mostafizur Rahman, Md. Shah Alam
Format: Article
Language:English
Published: Hasan Eleroğlu 2022-06-01
Series:Turkish Journal of Agriculture: Food Science and Technology
Subjects:
Online Access:http://www.agrifoodscience.com/index.php/TURJAF/article/view/4980
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1849471589295325184
author Shampa Rani
Khondoker Jahengir Alam
Shib Shankar Saha
Md. Mostafizur Rahman
Md. Shah Alam
author_facet Shampa Rani
Khondoker Jahengir Alam
Shib Shankar Saha
Md. Mostafizur Rahman
Md. Shah Alam
author_sort Shampa Rani
collection DOAJ
description Salmonellosis is a critical challenge in commercial poultry farming. This study aimed to calculate seroprevalence, identify Salmonella spp., and its pathological investigation from January to December 2019. One hundred fifty (150) serum samples and fifty (50) cloacal swabs from apparently ill and healthy birds were collected in this study. Seroprevalence was observed by serum plate agglutination (SPA) tests. The tentative diagnosis of salmonellosis was made based on history, clinical signs and bacteriological studies. Salmonella spp. was further confirmed using an automated microbiological method (VITEK®2). Post mortem examination was done in apparently sick birds, and gross and microscopic pathological lesions were investigated and recorded in a datasheet. The overall seroprevalence of salmonellosis was 42.67% in commercial chickens. Age wise prevalence of avian salmonellosis showed significantly highest infection rate in adult layers (≥ 45 days old) 65.31%, then 40.74%up to 45 days. In case of broiler, the prevalence rate in 0-10 days, 11-20 days, and 21-35 days were 13.63%, 44.12%, and 16.67%, respectively. Seasonal influence showed significantly highest proportionate prevalence of salmonellosis during summer 66.15%in comparison to winter 25.00% and rainy 24.44% seasons respectively. In bacteriological study, 12 samples were positive for Salmonellae among 50 cloacal samples. Out of 12 positive samples 5 samples were selected for the automated microbiology system VITEK® 2, and only two samples were confirmed as Salmonella gallinarum. Gross pathology of representative organs revealed bronze-colored enlarged liver, hemorrhages in the spleen and lungs, and hemorrhages in the ovary with stalk development. Microscopically, multifocal nodule formation and infiltration of inflammatory cells in the liver parenchyma and marked congestion with inflammatory cells in the spleen, hemorrhage and congestion in the lungs and intestine.
format Article
id doaj-art-e8678007e5ef4dc69a7a9796463765e9
institution Kabale University
issn 2148-127X
language English
publishDate 2022-06-01
publisher Hasan Eleroğlu
record_format Article
series Turkish Journal of Agriculture: Food Science and Technology
spelling doaj-art-e8678007e5ef4dc69a7a9796463765e92025-08-20T03:24:47ZengHasan EleroğluTurkish Journal of Agriculture: Food Science and Technology2148-127X2022-06-011061158116410.24925/turjaf.v10i6.1158-1164.49802518Seroprevalence, Identification, and Pathology of Salmonellosis in Selected Poultry Farms at Barishal District of BangladeshShampa Rani0Khondoker Jahengir Alam1Shib Shankar Saha2Md. Mostafizur Rahman3Md. Shah Alam4Faculty of Animal science and Veterinary Medicine, Patuakhali Science and Technology University, Barishal-8210Faculty of Animal science and Veterinary Medicine, Patuakhali Science and Technology University, Barishal-8210Faculty of Animal science and Veterinary Medicine, Patuakhali Science and Technology University, Barishal-8210Faculty of Animal science and Veterinary Medicine, Patuakhali Science and Technology University, Barishal-8210Faculty of Animal science and Veterinary Medicine, Patuakhali Science and Technology University, Barishal-8210Salmonellosis is a critical challenge in commercial poultry farming. This study aimed to calculate seroprevalence, identify Salmonella spp., and its pathological investigation from January to December 2019. One hundred fifty (150) serum samples and fifty (50) cloacal swabs from apparently ill and healthy birds were collected in this study. Seroprevalence was observed by serum plate agglutination (SPA) tests. The tentative diagnosis of salmonellosis was made based on history, clinical signs and bacteriological studies. Salmonella spp. was further confirmed using an automated microbiological method (VITEK®2). Post mortem examination was done in apparently sick birds, and gross and microscopic pathological lesions were investigated and recorded in a datasheet. The overall seroprevalence of salmonellosis was 42.67% in commercial chickens. Age wise prevalence of avian salmonellosis showed significantly highest infection rate in adult layers (≥ 45 days old) 65.31%, then 40.74%up to 45 days. In case of broiler, the prevalence rate in 0-10 days, 11-20 days, and 21-35 days were 13.63%, 44.12%, and 16.67%, respectively. Seasonal influence showed significantly highest proportionate prevalence of salmonellosis during summer 66.15%in comparison to winter 25.00% and rainy 24.44% seasons respectively. In bacteriological study, 12 samples were positive for Salmonellae among 50 cloacal samples. Out of 12 positive samples 5 samples were selected for the automated microbiology system VITEK® 2, and only two samples were confirmed as Salmonella gallinarum. Gross pathology of representative organs revealed bronze-colored enlarged liver, hemorrhages in the spleen and lungs, and hemorrhages in the ovary with stalk development. Microscopically, multifocal nodule formation and infiltration of inflammatory cells in the liver parenchyma and marked congestion with inflammatory cells in the spleen, hemorrhage and congestion in the lungs and intestine.http://www.agrifoodscience.com/index.php/TURJAF/article/view/4980salmonellosisprevalencepoultryisolationidentification
spellingShingle Shampa Rani
Khondoker Jahengir Alam
Shib Shankar Saha
Md. Mostafizur Rahman
Md. Shah Alam
Seroprevalence, Identification, and Pathology of Salmonellosis in Selected Poultry Farms at Barishal District of Bangladesh
Turkish Journal of Agriculture: Food Science and Technology
salmonellosis
prevalence
poultry
isolation
identification
title Seroprevalence, Identification, and Pathology of Salmonellosis in Selected Poultry Farms at Barishal District of Bangladesh
title_full Seroprevalence, Identification, and Pathology of Salmonellosis in Selected Poultry Farms at Barishal District of Bangladesh
title_fullStr Seroprevalence, Identification, and Pathology of Salmonellosis in Selected Poultry Farms at Barishal District of Bangladesh
title_full_unstemmed Seroprevalence, Identification, and Pathology of Salmonellosis in Selected Poultry Farms at Barishal District of Bangladesh
title_short Seroprevalence, Identification, and Pathology of Salmonellosis in Selected Poultry Farms at Barishal District of Bangladesh
title_sort seroprevalence identification and pathology of salmonellosis in selected poultry farms at barishal district of bangladesh
topic salmonellosis
prevalence
poultry
isolation
identification
url http://www.agrifoodscience.com/index.php/TURJAF/article/view/4980
work_keys_str_mv AT shamparani seroprevalenceidentificationandpathologyofsalmonellosisinselectedpoultryfarmsatbarishaldistrictofbangladesh
AT khondokerjahengiralam seroprevalenceidentificationandpathologyofsalmonellosisinselectedpoultryfarmsatbarishaldistrictofbangladesh
AT shibshankarsaha seroprevalenceidentificationandpathologyofsalmonellosisinselectedpoultryfarmsatbarishaldistrictofbangladesh
AT mdmostafizurrahman seroprevalenceidentificationandpathologyofsalmonellosisinselectedpoultryfarmsatbarishaldistrictofbangladesh
AT mdshahalam seroprevalenceidentificationandpathologyofsalmonellosisinselectedpoultryfarmsatbarishaldistrictofbangladesh