Interpretation of ecotoxicity tests using Enchytraeus albidus: Weight of soil characteristics in conditions of diffuse pollution
One way to evaluate the impact of chemical substances released in the ecosystems is the use of terrestrial organisms in ecotoxicological bioassays. Enchytraeus albidus (Oligochaeta) is a species commonly used in such standardized bioassays aiming at identifying biological responses as life traits (s...
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Elsevier
2025-03-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651325003227 |
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| author | Andrea Chacon-Hurtado Juliette Faburé Laura Sereni Sylvie Nélieu Nathalie Cheviron Ghislaine Delarue Emmanuelle Baudry Isabelle Lamy |
| author_facet | Andrea Chacon-Hurtado Juliette Faburé Laura Sereni Sylvie Nélieu Nathalie Cheviron Ghislaine Delarue Emmanuelle Baudry Isabelle Lamy |
| author_sort | Andrea Chacon-Hurtado |
| collection | DOAJ |
| description | One way to evaluate the impact of chemical substances released in the ecosystems is the use of terrestrial organisms in ecotoxicological bioassays. Enchytraeus albidus (Oligochaeta) is a species commonly used in such standardized bioassays aiming at identifying biological responses as life traits (survival and reproduction) using artificial spiked soils or in situ contaminated soils. However, in the latter case, it is known that other factors such as soil characteristics may affect the responses in E. albidus. We hypothesized that, in cases of low diffuse pollution, soil characteristics are major confounding factors that interfere with the interpretation of life trait responses in bioassays when their influence outweighs that of the diffuse pollution itself. To evaluate these confounding factors, 20 soils were collected from a low-contamination area encompassing diverse landscapes (urban, peri-urban, and rural) that exhibited gradients in pedological characteristics, biological parameters, and organic and inorganic contaminant levels, all within the range of classical values considered optimal for reproduction. E. albidus individuals were exposed to each soil sample, and survival and reproduction were monitored as life traits. Soil clustering and multifactorial analysis were employed to identify the confounding factors. Regardless of the life trait, low and sublethal contamination was rarely an explanatory factor for the responses of E. albidus. Survival was unaffected by soil characteristics or diffuse pollution. In contrast, reproduction was significantly and positively associated with soil pH and the levels of exchangeable sodium, calcium carbonate, molybdenum, PAHs, and insecticides, while it was negatively influenced by aluminum and exchangeable manganese. These findings suggest that studies conducted under conditions of diffuse, sublethal contamination should consider multiple natural soil characteristics in ecotoxicological bioassays to enable meaningful comparisons. |
| format | Article |
| id | doaj-art-e860b803ba974f68bbb9eb7ef71db5d1 |
| institution | DOAJ |
| issn | 0147-6513 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
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| series | Ecotoxicology and Environmental Safety |
| spelling | doaj-art-e860b803ba974f68bbb9eb7ef71db5d12025-08-20T02:52:27ZengElsevierEcotoxicology and Environmental Safety0147-65132025-03-0129211798610.1016/j.ecoenv.2025.117986Interpretation of ecotoxicity tests using Enchytraeus albidus: Weight of soil characteristics in conditions of diffuse pollutionAndrea Chacon-Hurtado0Juliette Faburé1Laura Sereni2Sylvie Nélieu3Nathalie Cheviron4Ghislaine Delarue5Emmanuelle Baudry6Isabelle Lamy7University Paris-Saclay, INRAE, AgroParisTech, UMR Ecosys, Palaiseau 91120, FranceUniversity Paris-Saclay, INRAE, AgroParisTech, UMR Ecosys, Palaiseau 91120, France; Corresponding author.University Paris-Saclay, INRAE, AgroParisTech, UMR Ecosys, Palaiseau 91120, FranceUniversity Paris-Saclay, INRAE, AgroParisTech, UMR Ecosys, Palaiseau 91120, FranceUniversity Paris-Saclay, INRAE, AgroParisTech, UMR Ecosys, Biochem-Env Platform, Palaiseau 91120, FranceUniversity Paris-Saclay, INRAE, AgroParisTech, UMR Ecosys, Palaiseau 91120, FranceUniversity Paris-Saclay, CNRS, AgroParisTech, Ecologie Systématique Evolution, Gif-sur-Yvette 91190, FranceUniversity Paris-Saclay, INRAE, AgroParisTech, UMR Ecosys, Palaiseau 91120, FranceOne way to evaluate the impact of chemical substances released in the ecosystems is the use of terrestrial organisms in ecotoxicological bioassays. Enchytraeus albidus (Oligochaeta) is a species commonly used in such standardized bioassays aiming at identifying biological responses as life traits (survival and reproduction) using artificial spiked soils or in situ contaminated soils. However, in the latter case, it is known that other factors such as soil characteristics may affect the responses in E. albidus. We hypothesized that, in cases of low diffuse pollution, soil characteristics are major confounding factors that interfere with the interpretation of life trait responses in bioassays when their influence outweighs that of the diffuse pollution itself. To evaluate these confounding factors, 20 soils were collected from a low-contamination area encompassing diverse landscapes (urban, peri-urban, and rural) that exhibited gradients in pedological characteristics, biological parameters, and organic and inorganic contaminant levels, all within the range of classical values considered optimal for reproduction. E. albidus individuals were exposed to each soil sample, and survival and reproduction were monitored as life traits. Soil clustering and multifactorial analysis were employed to identify the confounding factors. Regardless of the life trait, low and sublethal contamination was rarely an explanatory factor for the responses of E. albidus. Survival was unaffected by soil characteristics or diffuse pollution. In contrast, reproduction was significantly and positively associated with soil pH and the levels of exchangeable sodium, calcium carbonate, molybdenum, PAHs, and insecticides, while it was negatively influenced by aluminum and exchangeable manganese. These findings suggest that studies conducted under conditions of diffuse, sublethal contamination should consider multiple natural soil characteristics in ecotoxicological bioassays to enable meaningful comparisons.http://www.sciencedirect.com/science/article/pii/S0147651325003227EnchytraeidSoil confounding factorsBioavailabilityLife traitBiological modelField contamination |
| spellingShingle | Andrea Chacon-Hurtado Juliette Faburé Laura Sereni Sylvie Nélieu Nathalie Cheviron Ghislaine Delarue Emmanuelle Baudry Isabelle Lamy Interpretation of ecotoxicity tests using Enchytraeus albidus: Weight of soil characteristics in conditions of diffuse pollution Ecotoxicology and Environmental Safety Enchytraeid Soil confounding factors Bioavailability Life trait Biological model Field contamination |
| title | Interpretation of ecotoxicity tests using Enchytraeus albidus: Weight of soil characteristics in conditions of diffuse pollution |
| title_full | Interpretation of ecotoxicity tests using Enchytraeus albidus: Weight of soil characteristics in conditions of diffuse pollution |
| title_fullStr | Interpretation of ecotoxicity tests using Enchytraeus albidus: Weight of soil characteristics in conditions of diffuse pollution |
| title_full_unstemmed | Interpretation of ecotoxicity tests using Enchytraeus albidus: Weight of soil characteristics in conditions of diffuse pollution |
| title_short | Interpretation of ecotoxicity tests using Enchytraeus albidus: Weight of soil characteristics in conditions of diffuse pollution |
| title_sort | interpretation of ecotoxicity tests using enchytraeus albidus weight of soil characteristics in conditions of diffuse pollution |
| topic | Enchytraeid Soil confounding factors Bioavailability Life trait Biological model Field contamination |
| url | http://www.sciencedirect.com/science/article/pii/S0147651325003227 |
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