Engineering Nicotiana benthamiana for chrysoeriol production using synthetic biology approaches
Flavonoids are prevalent plant secondary metabolites with a broad range of biological activities. Their antioxidant, anti-inflammatory, and anti-cancer activities make flavonoids widely useful in a variety of industries, including the pharmaceutical and health food industries. However, many flavonoi...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-12-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2024.1458916/full |
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| author | Saet Buyl Lee Sung-eun Lee Hyo Lee Ji-Su Kim Hyoseon Choi Sichul Lee Beom-Gi Kim |
| author_facet | Saet Buyl Lee Sung-eun Lee Hyo Lee Ji-Su Kim Hyoseon Choi Sichul Lee Beom-Gi Kim |
| author_sort | Saet Buyl Lee |
| collection | DOAJ |
| description | Flavonoids are prevalent plant secondary metabolites with a broad range of biological activities. Their antioxidant, anti-inflammatory, and anti-cancer activities make flavonoids widely useful in a variety of industries, including the pharmaceutical and health food industries. However, many flavonoids occur at only low concentrations in plants, and they are difficult to synthesize chemically due to their structural complexity. To address these difficulties, new technologies have been employed to enhance the production of flavonoids in vivo. In this study, we used synthetic biology techniques to produce the methylated flavone chrysoeriol in Nicotiana benthamiana leaves. The chrysoeriol biosynthetic pathway consists of eight catalytic steps. However, using an Agrobacterium-mediated transient expression assay to examine the in planta activities of genes of interest, we shortened this pathway to four steps catalyzed by five enzymes. Co-expression of these five enzymes in N. benthamiana leaves resulted in de novo chrysoeriol production. Chrysoeriol production was unaffected by the Agrobacterium cell density used for agroinfiltration and increased over time, peaking at 10 days after infiltration. Chrysoeriol accumulation in agroinfiltrated N. benthamiana leaves was associated with increased antioxidant activity, a typical property of flavones. Taken together, our results demonstrate that synthetic biology represents a practical method for engineering plants to produce substantial amounts of flavonoids and flavonoid derivatives without the need for exogenous substrates. |
| format | Article |
| id | doaj-art-e860a8f5c3f04d109db6a6b8e03ab207 |
| institution | OA Journals |
| issn | 1664-462X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj-art-e860a8f5c3f04d109db6a6b8e03ab2072025-08-20T02:36:54ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2024-12-011510.3389/fpls.2024.14589161458916Engineering Nicotiana benthamiana for chrysoeriol production using synthetic biology approachesSaet Buyl LeeSung-eun LeeHyo LeeJi-Su KimHyoseon ChoiSichul LeeBeom-Gi KimFlavonoids are prevalent plant secondary metabolites with a broad range of biological activities. Their antioxidant, anti-inflammatory, and anti-cancer activities make flavonoids widely useful in a variety of industries, including the pharmaceutical and health food industries. However, many flavonoids occur at only low concentrations in plants, and they are difficult to synthesize chemically due to their structural complexity. To address these difficulties, new technologies have been employed to enhance the production of flavonoids in vivo. In this study, we used synthetic biology techniques to produce the methylated flavone chrysoeriol in Nicotiana benthamiana leaves. The chrysoeriol biosynthetic pathway consists of eight catalytic steps. However, using an Agrobacterium-mediated transient expression assay to examine the in planta activities of genes of interest, we shortened this pathway to four steps catalyzed by five enzymes. Co-expression of these five enzymes in N. benthamiana leaves resulted in de novo chrysoeriol production. Chrysoeriol production was unaffected by the Agrobacterium cell density used for agroinfiltration and increased over time, peaking at 10 days after infiltration. Chrysoeriol accumulation in agroinfiltrated N. benthamiana leaves was associated with increased antioxidant activity, a typical property of flavones. Taken together, our results demonstrate that synthetic biology represents a practical method for engineering plants to produce substantial amounts of flavonoids and flavonoid derivatives without the need for exogenous substrates.https://www.frontiersin.org/articles/10.3389/fpls.2024.1458916/fullchrysoeriolco-expressionflavonoidgene combinationNicotiana benthamianareconstruction |
| spellingShingle | Saet Buyl Lee Sung-eun Lee Hyo Lee Ji-Su Kim Hyoseon Choi Sichul Lee Beom-Gi Kim Engineering Nicotiana benthamiana for chrysoeriol production using synthetic biology approaches Frontiers in Plant Science chrysoeriol co-expression flavonoid gene combination Nicotiana benthamiana reconstruction |
| title | Engineering Nicotiana benthamiana for chrysoeriol production using synthetic biology approaches |
| title_full | Engineering Nicotiana benthamiana for chrysoeriol production using synthetic biology approaches |
| title_fullStr | Engineering Nicotiana benthamiana for chrysoeriol production using synthetic biology approaches |
| title_full_unstemmed | Engineering Nicotiana benthamiana for chrysoeriol production using synthetic biology approaches |
| title_short | Engineering Nicotiana benthamiana for chrysoeriol production using synthetic biology approaches |
| title_sort | engineering nicotiana benthamiana for chrysoeriol production using synthetic biology approaches |
| topic | chrysoeriol co-expression flavonoid gene combination Nicotiana benthamiana reconstruction |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2024.1458916/full |
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