Expression of bcl-2 in the liver tissue of patients with autoimmune liver diseases and chronic hepatitis C
Changes of apoptosis in liver cells are observed in patients with lesions of various etiologies. The aim of this study was a comparative evaluation of the expression of the antiapoptotic factor bcl-2 in liver at CHC and autoimmune liver diseases (AiLD). Immunohistochemical assessment of bcl -2 expre...
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| Format: | Article |
| Language: | Russian |
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Journal Infectology
2014-09-01
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| Series: | Журнал инфектологии |
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| Online Access: | https://journal.niidi.ru/jofin/article/view/195 |
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| author | K. L. Raykhel`son V. E. Karev N. V. Marchenko L. K. Pal`gova Yu. V. Lobzin A. Yu. Baranovskiy |
| author_facet | K. L. Raykhel`son V. E. Karev N. V. Marchenko L. K. Pal`gova Yu. V. Lobzin A. Yu. Baranovskiy |
| author_sort | K. L. Raykhel`son |
| collection | DOAJ |
| description | Changes of apoptosis in liver cells are observed in patients with lesions of various etiologies. The aim of this study was a comparative evaluation of the expression of the antiapoptotic factor bcl-2 in liver at CHC and autoimmune liver diseases (AiLD). Immunohistochemical assessment of bcl -2 expression in biliary epithelial cells and non-parenchymal cells were performed in liver biopsies from 33 patients with liver disease (7 – with autoimmune hepatitis (AIH), 10 – with primary biliary cirrhosis (PBC), 6 – overlap syndrome AIH / PBC and 10 – with chronic hepatitis (CHC)). In all groups, the greatest density of bcl-2+ non-parenchymal cells showed in the portal area, moreover, the expression of bcl-2 in non-parenchymal cells at CHC patients was significantly lower than in the other groups. The interrelation between necroinflammatory activity and bcl-2 expression in non-parenchymal liver cells was found (r = 0,35), the relationship with the stage of fibrosis was not found. We were obtained statistically significant differences in bcl-2 expression in biliary epithelial cells by semi-quantitative assessment: in the majority of patients with PBC bcl-2 was expressed over 2/3 of cholangiocytes, at AIH and AIH/PBC patients bcl-2+ reaction was observed in 1/3–2/3 biliary cells, and all CHC patients had bcl-2 expression less than in 1/3 cholangiocytes (p <0.05). Differences between AIH and AIH/PBC of bcl-2 expression in cholangiocytes were not observed. The intensity of bcl-2 expression in biliary epithelial cells can be used as a differential diagnostic marker when necessary to differentiate AIH and CHC in controversial situations and, most importantly, AIH / PBC and PBC. |
| format | Article |
| id | doaj-art-e8609f5efcf8463d93ad3b351350827a |
| institution | DOAJ |
| issn | 2072-6732 |
| language | Russian |
| publishDate | 2014-09-01 |
| publisher | Journal Infectology |
| record_format | Article |
| series | Журнал инфектологии |
| spelling | doaj-art-e8609f5efcf8463d93ad3b351350827a2025-08-20T03:22:15ZrusJournal InfectologyЖурнал инфектологии2072-67322014-09-0154141910.22625/2072-6732-2013-5-4-14-19209Expression of bcl-2 in the liver tissue of patients with autoimmune liver diseases and chronic hepatitis CK. L. Raykhel`son0V. E. Karev1N. V. Marchenko2L. K. Pal`gova3Yu. V. Lobzin4A. Yu. Baranovskiy5North-Western State Medical University named after I.I. Mechnikov, Saint-PetersburgScientific Research Institute of Children`s Infections of FMBA of Russia, Saint-PetersburgNorth-Western State Medical University named after I.I. Mechnikov, Saint-PetersburgNorth-Western State Medical University named after I.I. Mechnikov, Saint-PetersburgScientific Research Institute of Children`s Infections of FMBA of Russia, Saint-PetersburgNorth-Western State Medical University named after I.I. Mechnikov, Saint-PetersburgChanges of apoptosis in liver cells are observed in patients with lesions of various etiologies. The aim of this study was a comparative evaluation of the expression of the antiapoptotic factor bcl-2 in liver at CHC and autoimmune liver diseases (AiLD). Immunohistochemical assessment of bcl -2 expression in biliary epithelial cells and non-parenchymal cells were performed in liver biopsies from 33 patients with liver disease (7 – with autoimmune hepatitis (AIH), 10 – with primary biliary cirrhosis (PBC), 6 – overlap syndrome AIH / PBC and 10 – with chronic hepatitis (CHC)). In all groups, the greatest density of bcl-2+ non-parenchymal cells showed in the portal area, moreover, the expression of bcl-2 in non-parenchymal cells at CHC patients was significantly lower than in the other groups. The interrelation between necroinflammatory activity and bcl-2 expression in non-parenchymal liver cells was found (r = 0,35), the relationship with the stage of fibrosis was not found. We were obtained statistically significant differences in bcl-2 expression in biliary epithelial cells by semi-quantitative assessment: in the majority of patients with PBC bcl-2 was expressed over 2/3 of cholangiocytes, at AIH and AIH/PBC patients bcl-2+ reaction was observed in 1/3–2/3 biliary cells, and all CHC patients had bcl-2 expression less than in 1/3 cholangiocytes (p <0.05). Differences between AIH and AIH/PBC of bcl-2 expression in cholangiocytes were not observed. The intensity of bcl-2 expression in biliary epithelial cells can be used as a differential diagnostic marker when necessary to differentiate AIH and CHC in controversial situations and, most importantly, AIH / PBC and PBC.https://journal.niidi.ru/jofin/article/view/195autoimmune hepatitisprimary biliary cirrhosischronic hepatitis coverlap syndromeapoptosisbcl-2differential diagnosis |
| spellingShingle | K. L. Raykhel`son V. E. Karev N. V. Marchenko L. K. Pal`gova Yu. V. Lobzin A. Yu. Baranovskiy Expression of bcl-2 in the liver tissue of patients with autoimmune liver diseases and chronic hepatitis C Журнал инфектологии autoimmune hepatitis primary biliary cirrhosis chronic hepatitis c overlap syndrome apoptosis bcl-2 differential diagnosis |
| title | Expression of bcl-2 in the liver tissue of patients with autoimmune liver diseases and chronic hepatitis C |
| title_full | Expression of bcl-2 in the liver tissue of patients with autoimmune liver diseases and chronic hepatitis C |
| title_fullStr | Expression of bcl-2 in the liver tissue of patients with autoimmune liver diseases and chronic hepatitis C |
| title_full_unstemmed | Expression of bcl-2 in the liver tissue of patients with autoimmune liver diseases and chronic hepatitis C |
| title_short | Expression of bcl-2 in the liver tissue of patients with autoimmune liver diseases and chronic hepatitis C |
| title_sort | expression of bcl 2 in the liver tissue of patients with autoimmune liver diseases and chronic hepatitis c |
| topic | autoimmune hepatitis primary biliary cirrhosis chronic hepatitis c overlap syndrome apoptosis bcl-2 differential diagnosis |
| url | https://journal.niidi.ru/jofin/article/view/195 |
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