Evaluation of antimicrobial susceptibilities and virulence factors of Staphylococcus aureus strains isolated from community-acquired and health-care associated pediatric infections
The aim of this study was to investigate the enterotoxins and Panton-Valentine leukocidin (PVL) gene as virulence factor, identification if antimicrobial sensitivity patterns, agr (accessory gene regulator) types and sequence types and in resistant cases to obtain SCCmec (staphylococcal cassette ch...
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Hacettepe University Institute of Child Health
2017-08-01
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| Series: | The Turkish Journal of Pediatrics |
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| Online Access: | https://turkjpediatr.org/article/view/1006 |
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| author | Adem Karbuz Zeynep Ceren Karahan Bilge Aldemir-Kocabaş Alper Tekeli Halil Özdemir Haluk Güriz Refik Gökdemir Erdal İnce Ergin Çiftçi |
| author_facet | Adem Karbuz Zeynep Ceren Karahan Bilge Aldemir-Kocabaş Alper Tekeli Halil Özdemir Haluk Güriz Refik Gökdemir Erdal İnce Ergin Çiftçi |
| author_sort | Adem Karbuz |
| collection | DOAJ |
| description |
The aim of this study was to investigate the enterotoxins and Panton-Valentine leukocidin (PVL) gene as virulence factor, identification if antimicrobial sensitivity patterns, agr (accessory gene regulator) types and sequence types and in resistant cases to obtain SCCmec (staphylococcal cassette chromosome mec) gene types which will be helpful to decide empirical therapy and future health politics for S. aureus species. Total of 150 isolates of S. aureus were isolated from the cultures of the child patients in January 2011 and December 2012. In this study, the penicillin resistance was observed as 93.8%. PVL and mecA was detected positive in 8.7% and in 6% of all S. aureus strains, respectively. Two MRSA (methicillin resistant S.aureus) strains were detected as SCCmec type III and SCCmec type V and five MRSA strains were detected as SCCmec type IV. SET-I and SET-G were the most common detected enterotoxins. In both community-associated and healthcare-associated MRSA strains, agr type 1 was detected most commonly. The most common sequence types were ST737 in 13 patients than ST22 in eight patients and ST121 in six patients. This study highlights a necessity to review the cause of small changes in the structural genes in order to determine whether it is a cause or outcome; community-acquired and healthcare associated strains overlap.
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| format | Article |
| id | doaj-art-e85f88b0af1b409c82bc34ed76fc2cd3 |
| institution | DOAJ |
| issn | 0041-4301 2791-6421 |
| language | English |
| publishDate | 2017-08-01 |
| publisher | Hacettepe University Institute of Child Health |
| record_format | Article |
| series | The Turkish Journal of Pediatrics |
| spelling | doaj-art-e85f88b0af1b409c82bc34ed76fc2cd32025-08-20T03:01:14ZengHacettepe University Institute of Child HealthThe Turkish Journal of Pediatrics0041-43012791-64212017-08-0159410.24953/turkjped.2017.04.005Evaluation of antimicrobial susceptibilities and virulence factors of Staphylococcus aureus strains isolated from community-acquired and health-care associated pediatric infectionsAdem Karbuz0Zeynep Ceren Karahan1Bilge Aldemir-Kocabaş2Alper Tekeli3Halil Özdemir4Haluk Güriz5Refik Gökdemir6Erdal İnce7Ergin Çiftçi8Departments of Pediatric Infectious Diseases, Ankara University Faculty of Medicine, Ankara, Turkey.Departments of Clinical and Basic Microbiology, Ankara University Faculty of Medicine, Ankara, Turkey.Departments of Pediatric Infectious Diseases, Ankara University Faculty of Medicine, Ankara, Turkey.Departments of Clinical and Basic Microbiology, Ankara University Faculty of Medicine, Ankara, Turkey.Departments of Pediatric Infectious Diseases, Ankara University Faculty of Medicine, Ankara, Turkey.Departments of Basic Microbiology, Ankara University Faculty of Medicine, Ankara, Turkey.Departments of Basic Microbiology, Ankara University Faculty of Medicine, Ankara, Turkey.Departments of Pediatric Infectious Diseases, Ankara University Faculty of Medicine, Ankara, Turkey.Departments of Pediatric Infectious Diseases, Ankara University Faculty of Medicine, Ankara, Turkey. The aim of this study was to investigate the enterotoxins and Panton-Valentine leukocidin (PVL) gene as virulence factor, identification if antimicrobial sensitivity patterns, agr (accessory gene regulator) types and sequence types and in resistant cases to obtain SCCmec (staphylococcal cassette chromosome mec) gene types which will be helpful to decide empirical therapy and future health politics for S. aureus species. Total of 150 isolates of S. aureus were isolated from the cultures of the child patients in January 2011 and December 2012. In this study, the penicillin resistance was observed as 93.8%. PVL and mecA was detected positive in 8.7% and in 6% of all S. aureus strains, respectively. Two MRSA (methicillin resistant S.aureus) strains were detected as SCCmec type III and SCCmec type V and five MRSA strains were detected as SCCmec type IV. SET-I and SET-G were the most common detected enterotoxins. In both community-associated and healthcare-associated MRSA strains, agr type 1 was detected most commonly. The most common sequence types were ST737 in 13 patients than ST22 in eight patients and ST121 in six patients. This study highlights a necessity to review the cause of small changes in the structural genes in order to determine whether it is a cause or outcome; community-acquired and healthcare associated strains overlap. https://turkjpediatr.org/article/view/1006Staphylococcus aureuspediatric infectionspulsed field gel electrophoresis profilevirulence factors |
| spellingShingle | Adem Karbuz Zeynep Ceren Karahan Bilge Aldemir-Kocabaş Alper Tekeli Halil Özdemir Haluk Güriz Refik Gökdemir Erdal İnce Ergin Çiftçi Evaluation of antimicrobial susceptibilities and virulence factors of Staphylococcus aureus strains isolated from community-acquired and health-care associated pediatric infections The Turkish Journal of Pediatrics Staphylococcus aureus pediatric infections pulsed field gel electrophoresis profile virulence factors |
| title | Evaluation of antimicrobial susceptibilities and virulence factors of Staphylococcus aureus strains isolated from community-acquired and health-care associated pediatric infections |
| title_full | Evaluation of antimicrobial susceptibilities and virulence factors of Staphylococcus aureus strains isolated from community-acquired and health-care associated pediatric infections |
| title_fullStr | Evaluation of antimicrobial susceptibilities and virulence factors of Staphylococcus aureus strains isolated from community-acquired and health-care associated pediatric infections |
| title_full_unstemmed | Evaluation of antimicrobial susceptibilities and virulence factors of Staphylococcus aureus strains isolated from community-acquired and health-care associated pediatric infections |
| title_short | Evaluation of antimicrobial susceptibilities and virulence factors of Staphylococcus aureus strains isolated from community-acquired and health-care associated pediatric infections |
| title_sort | evaluation of antimicrobial susceptibilities and virulence factors of staphylococcus aureus strains isolated from community acquired and health care associated pediatric infections |
| topic | Staphylococcus aureus pediatric infections pulsed field gel electrophoresis profile virulence factors |
| url | https://turkjpediatr.org/article/view/1006 |
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