Effects of Alprostadil on experimental peritoneal fibrosis in rats
Objective To investigate the effects and possible action mechanism of Alprostadil in the treatment of experimental peritoneal fibrosis in rats.Methods The rat peritoneal fibrosis model was induced by high glucose dialysate and lipopolysaccharide(LPS).The rats were randomly divided into five groups:c...
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Editorial Department of Journal of Clinical Nephrology
2015-01-01
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| Series: | Linchuang shenzangbing zazhi |
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| Online Access: | http://www.lcszb.com/thesisDetails?columnId=57920730&Fpath=home&index=0 |
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| author | ZHANG Yan-min CHEN Wei-dong WAN Sheng CHEN Dan CHEN Fang DING Yan-qiong WANG Zeng-si TIAN Hong-dan XIONG Fei |
| author_facet | ZHANG Yan-min CHEN Wei-dong WAN Sheng CHEN Dan CHEN Fang DING Yan-qiong WANG Zeng-si TIAN Hong-dan XIONG Fei |
| author_sort | ZHANG Yan-min |
| collection | DOAJ |
| description | Objective To investigate the effects and possible action mechanism of Alprostadil in the treatment of experimental peritoneal fibrosis in rats.Methods The rat peritoneal fibrosis model was induced by high glucose dialysate and lipopolysaccharide(LPS).The rats were randomly divided into five groups:control group,model group,low-dose Alprostadil-treated group,middle-dose Alprostadil-treated group and high-dose Alprostadil-treated group.The Alprostadil-treated groups received daily intraperitoneal injection of different doses of Alprostadil.Six weeks later all rats were anaesthetized.The peritoneal membrane function was investigated by peritoneal equilibration test(PET).The abdominal membrane was stained with HE and Masson method.The levels of TGF-β and TLR4 in peritoneum were detected by immunohistochemical method.Results The number of vascularity in model group was greater than that in Alprostadil-treated groups.The concentrations of TGFβand TLR4 in blood and dialysate were also decreased obviously in Alprostadil-treated groups.The levels of TGFβ and TLR4 in peritoneumin in Alprostadil-treated groups were significantly lower than those in model group.Conclusions Alprostadil are beneficial to the prevention and treatment of peritoneal fibrosis in experimental model rats. |
| format | Article |
| id | doaj-art-e84eec2f22b345c5b37d23915dd522a5 |
| institution | OA Journals |
| issn | 1671-2390 |
| language | zho |
| publishDate | 2015-01-01 |
| publisher | Editorial Department of Journal of Clinical Nephrology |
| record_format | Article |
| series | Linchuang shenzangbing zazhi |
| spelling | doaj-art-e84eec2f22b345c5b37d23915dd522a52025-08-20T02:16:10ZzhoEditorial Department of Journal of Clinical NephrologyLinchuang shenzangbing zazhi1671-23902015-01-0149750157920730Effects of Alprostadil on experimental peritoneal fibrosis in ratsZHANG Yan-minCHEN Wei-dongWAN ShengCHEN DanCHEN FangDING Yan-qiongWANG Zeng-siTIAN Hong-danXIONG FeiObjective To investigate the effects and possible action mechanism of Alprostadil in the treatment of experimental peritoneal fibrosis in rats.Methods The rat peritoneal fibrosis model was induced by high glucose dialysate and lipopolysaccharide(LPS).The rats were randomly divided into five groups:control group,model group,low-dose Alprostadil-treated group,middle-dose Alprostadil-treated group and high-dose Alprostadil-treated group.The Alprostadil-treated groups received daily intraperitoneal injection of different doses of Alprostadil.Six weeks later all rats were anaesthetized.The peritoneal membrane function was investigated by peritoneal equilibration test(PET).The abdominal membrane was stained with HE and Masson method.The levels of TGF-β and TLR4 in peritoneum were detected by immunohistochemical method.Results The number of vascularity in model group was greater than that in Alprostadil-treated groups.The concentrations of TGFβand TLR4 in blood and dialysate were also decreased obviously in Alprostadil-treated groups.The levels of TGFβ and TLR4 in peritoneumin in Alprostadil-treated groups were significantly lower than those in model group.Conclusions Alprostadil are beneficial to the prevention and treatment of peritoneal fibrosis in experimental model rats.http://www.lcszb.com/thesisDetails?columnId=57920730&Fpath=home&index=0Peritoneal fibrosisAlprostadilTransforming growth factor-βToll like receptor 4 |
| spellingShingle | ZHANG Yan-min CHEN Wei-dong WAN Sheng CHEN Dan CHEN Fang DING Yan-qiong WANG Zeng-si TIAN Hong-dan XIONG Fei Effects of Alprostadil on experimental peritoneal fibrosis in rats Linchuang shenzangbing zazhi Peritoneal fibrosis Alprostadil Transforming growth factor-β Toll like receptor 4 |
| title | Effects of Alprostadil on experimental peritoneal fibrosis in rats |
| title_full | Effects of Alprostadil on experimental peritoneal fibrosis in rats |
| title_fullStr | Effects of Alprostadil on experimental peritoneal fibrosis in rats |
| title_full_unstemmed | Effects of Alprostadil on experimental peritoneal fibrosis in rats |
| title_short | Effects of Alprostadil on experimental peritoneal fibrosis in rats |
| title_sort | effects of alprostadil on experimental peritoneal fibrosis in rats |
| topic | Peritoneal fibrosis Alprostadil Transforming growth factor-β Toll like receptor 4 |
| url | http://www.lcszb.com/thesisDetails?columnId=57920730&Fpath=home&index=0 |
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