Microbicidal Activity of Extract <i>Larrea tridentata</i> (Sessé and Moc. ex DC.) Coville on <i>Pseudomonas syringae</i> Van Hall and <i>Botrytis cinerea</i> Pers
Due to their secondary metabolite content, plant extracts are an alternative method for controlling pathogenic organisms in agriculture and post-harvest operations. <i>Botrytis cinerea</i> and <i>Pseudomonas syringae</i> are among the causative agents of diseases and losses i...
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MDPI AG
2025-04-01
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| Series: | Microorganisms |
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| author | Diego Rivera-Escareño Jorge Cadena-Iñiguez Dalia Abigail García-Flores Gerardo Loera-Alvarado Lizeth Aguilar-Galaviz María Azucena Ortega-Amaro |
| author_facet | Diego Rivera-Escareño Jorge Cadena-Iñiguez Dalia Abigail García-Flores Gerardo Loera-Alvarado Lizeth Aguilar-Galaviz María Azucena Ortega-Amaro |
| author_sort | Diego Rivera-Escareño |
| collection | DOAJ |
| description | Due to their secondary metabolite content, plant extracts are an alternative method for controlling pathogenic organisms in agriculture and post-harvest operations. <i>Botrytis cinerea</i> and <i>Pseudomonas syringae</i> are among the causative agents of diseases and losses in agricultural production. The species <i>Larrea tridentata</i> is abundant in the arid and semi-arid zones of Mexico and has no defined use; however, it contains secondary metabolites with microbicidal potential that could aid in biological control and enhance its harvest status. Growth inhibition (halo) of <i>B. cinerea</i> and <i>P. syringae</i> was evaluated by applying alcoholic extract of <i>L. tridentata</i> leaves at doses of 50, 100, 250, 500, 750, 1000, and 2000 µg mL<sup>−1</sup> in vitro, using poisoned medium and potato dextrose agar for the fungus and the agar well method for the bacteria, in a completely randomized design with five replicates. The flavonoids quercetin, apigenin, narigenin, kaempferol, and galangin were identified as possible agents of microbicidal activity. The extract inhibited the growth of <i>B. cinerea</i> from 100 µg mL<sup>−1</sup> and completely inhibited it with 1000 and 2000 µg mL<sup>−1</sup>. For <i>P. syringae</i>, inhibition was observed from 250 µg mL<sup>−1</sup>, demonstrating that the higher the concentration, the greater the growth inhibitory effect. The secondary metabolite content of the <i>L. tridentata</i> extract is sufficient to have an impact on microorganisms with economic impact in agriculture. |
| format | Article |
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| institution | OA Journals |
| issn | 2076-2607 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj-art-e8493baa46d241df88f240a018dcae832025-08-20T02:33:51ZengMDPI AGMicroorganisms2076-26072025-04-01135105510.3390/microorganisms13051055Microbicidal Activity of Extract <i>Larrea tridentata</i> (Sessé and Moc. ex DC.) Coville on <i>Pseudomonas syringae</i> Van Hall and <i>Botrytis cinerea</i> PersDiego Rivera-Escareño0Jorge Cadena-Iñiguez1Dalia Abigail García-Flores2Gerardo Loera-Alvarado3Lizeth Aguilar-Galaviz4María Azucena Ortega-Amaro5Colegio de Postgraduados, Innovación en Manejo de Recursos Naturales, Campus San Luis Potosí, Iturbide 73, Salinas de Hidalgo C.P. 78600, SLP, MexicoColegio de Postgraduados, Innovación en Manejo de Recursos Naturales, Campus San Luis Potosí, Iturbide 73, Salinas de Hidalgo C.P. 78600, SLP, MexicoColegio de Postgraduados, Innovación en Manejo de Recursos Naturales, Campus San Luis Potosí, Iturbide 73, Salinas de Hidalgo C.P. 78600, SLP, MexicoColegio de Postgraduados, Innovación en Manejo de Recursos Naturales, Campus San Luis Potosí, Iturbide 73, Salinas de Hidalgo C.P. 78600, SLP, MexicoColegio de Postgraduados, Innovación en Manejo de Recursos Naturales, Campus San Luis Potosí, Iturbide 73, Salinas de Hidalgo C.P. 78600, SLP, MexicoCoordinación Académica Región Altiplano Oeste, Universidad Autónoma de San Luis Potosí, Carretera Salinas-Santo Domingo 200, Salinas de Hidalgo C.P. 78600, SLP, MexicoDue to their secondary metabolite content, plant extracts are an alternative method for controlling pathogenic organisms in agriculture and post-harvest operations. <i>Botrytis cinerea</i> and <i>Pseudomonas syringae</i> are among the causative agents of diseases and losses in agricultural production. The species <i>Larrea tridentata</i> is abundant in the arid and semi-arid zones of Mexico and has no defined use; however, it contains secondary metabolites with microbicidal potential that could aid in biological control and enhance its harvest status. Growth inhibition (halo) of <i>B. cinerea</i> and <i>P. syringae</i> was evaluated by applying alcoholic extract of <i>L. tridentata</i> leaves at doses of 50, 100, 250, 500, 750, 1000, and 2000 µg mL<sup>−1</sup> in vitro, using poisoned medium and potato dextrose agar for the fungus and the agar well method for the bacteria, in a completely randomized design with five replicates. The flavonoids quercetin, apigenin, narigenin, kaempferol, and galangin were identified as possible agents of microbicidal activity. The extract inhibited the growth of <i>B. cinerea</i> from 100 µg mL<sup>−1</sup> and completely inhibited it with 1000 and 2000 µg mL<sup>−1</sup>. For <i>P. syringae</i>, inhibition was observed from 250 µg mL<sup>−1</sup>, demonstrating that the higher the concentration, the greater the growth inhibitory effect. The secondary metabolite content of the <i>L. tridentata</i> extract is sufficient to have an impact on microorganisms with economic impact in agriculture.https://www.mdpi.com/2076-2607/13/5/1055phytochemicalscrop diseasesbotanical extractssustainable control |
| spellingShingle | Diego Rivera-Escareño Jorge Cadena-Iñiguez Dalia Abigail García-Flores Gerardo Loera-Alvarado Lizeth Aguilar-Galaviz María Azucena Ortega-Amaro Microbicidal Activity of Extract <i>Larrea tridentata</i> (Sessé and Moc. ex DC.) Coville on <i>Pseudomonas syringae</i> Van Hall and <i>Botrytis cinerea</i> Pers Microorganisms phytochemicals crop diseases botanical extracts sustainable control |
| title | Microbicidal Activity of Extract <i>Larrea tridentata</i> (Sessé and Moc. ex DC.) Coville on <i>Pseudomonas syringae</i> Van Hall and <i>Botrytis cinerea</i> Pers |
| title_full | Microbicidal Activity of Extract <i>Larrea tridentata</i> (Sessé and Moc. ex DC.) Coville on <i>Pseudomonas syringae</i> Van Hall and <i>Botrytis cinerea</i> Pers |
| title_fullStr | Microbicidal Activity of Extract <i>Larrea tridentata</i> (Sessé and Moc. ex DC.) Coville on <i>Pseudomonas syringae</i> Van Hall and <i>Botrytis cinerea</i> Pers |
| title_full_unstemmed | Microbicidal Activity of Extract <i>Larrea tridentata</i> (Sessé and Moc. ex DC.) Coville on <i>Pseudomonas syringae</i> Van Hall and <i>Botrytis cinerea</i> Pers |
| title_short | Microbicidal Activity of Extract <i>Larrea tridentata</i> (Sessé and Moc. ex DC.) Coville on <i>Pseudomonas syringae</i> Van Hall and <i>Botrytis cinerea</i> Pers |
| title_sort | microbicidal activity of extract i larrea tridentata i sesse and moc ex dc coville on i pseudomonas syringae i van hall and i botrytis cinerea i pers |
| topic | phytochemicals crop diseases botanical extracts sustainable control |
| url | https://www.mdpi.com/2076-2607/13/5/1055 |
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