Microbicidal Activity of Extract <i>Larrea tridentata</i> (Sessé and Moc. ex DC.) Coville on <i>Pseudomonas syringae</i> Van Hall and <i>Botrytis cinerea</i> Pers
Due to their secondary metabolite content, plant extracts are an alternative method for controlling pathogenic organisms in agriculture and post-harvest operations. <i>Botrytis cinerea</i> and <i>Pseudomonas syringae</i> are among the causative agents of diseases and losses i...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-04-01
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| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/13/5/1055 |
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| Summary: | Due to their secondary metabolite content, plant extracts are an alternative method for controlling pathogenic organisms in agriculture and post-harvest operations. <i>Botrytis cinerea</i> and <i>Pseudomonas syringae</i> are among the causative agents of diseases and losses in agricultural production. The species <i>Larrea tridentata</i> is abundant in the arid and semi-arid zones of Mexico and has no defined use; however, it contains secondary metabolites with microbicidal potential that could aid in biological control and enhance its harvest status. Growth inhibition (halo) of <i>B. cinerea</i> and <i>P. syringae</i> was evaluated by applying alcoholic extract of <i>L. tridentata</i> leaves at doses of 50, 100, 250, 500, 750, 1000, and 2000 µg mL<sup>−1</sup> in vitro, using poisoned medium and potato dextrose agar for the fungus and the agar well method for the bacteria, in a completely randomized design with five replicates. The flavonoids quercetin, apigenin, narigenin, kaempferol, and galangin were identified as possible agents of microbicidal activity. The extract inhibited the growth of <i>B. cinerea</i> from 100 µg mL<sup>−1</sup> and completely inhibited it with 1000 and 2000 µg mL<sup>−1</sup>. For <i>P. syringae</i>, inhibition was observed from 250 µg mL<sup>−1</sup>, demonstrating that the higher the concentration, the greater the growth inhibitory effect. The secondary metabolite content of the <i>L. tridentata</i> extract is sufficient to have an impact on microorganisms with economic impact in agriculture. |
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| ISSN: | 2076-2607 |