Mannan-oligosaccharide modulates gut microbiota and enhances immune-metabolic functions in Chinese mitten crab (Eriocheir sinensis): Insights from an in vitro fermentation model
Prebiotics are promising alternatives to antibiotics for enhancing disease resistance in aquaculture, yet their immunomodulatory mechanisms remain poorly understood.This study investigated the regulatory effects of prebiotics through in vitro experiments by directly comparing the effects of seven pr...
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| Language: | English |
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Elsevier
2025-09-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513425003448 |
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| author | Wei Li Shubin Liu Kaiqi Huang Xiaoling Zhang Xiaodan Wang Jian G. Qin Liqiao Chen Erchao Li |
| author_facet | Wei Li Shubin Liu Kaiqi Huang Xiaoling Zhang Xiaodan Wang Jian G. Qin Liqiao Chen Erchao Li |
| author_sort | Wei Li |
| collection | DOAJ |
| description | Prebiotics are promising alternatives to antibiotics for enhancing disease resistance in aquaculture, yet their immunomodulatory mechanisms remain poorly understood.This study investigated the regulatory effects of prebiotics through in vitro experiments by directly comparing the effects of seven prebiotics, fructo-oligosaccharides (FOSs), galacto-oligosaccharides (GOSs), Mannan-oligosaccharides (MOSs), inulin, β-glucan, isomaltodextrin (IMO), and xylo-oligosaccharides (XOSs), on the gut microbiota and metabolites in the Chinese Mitten Crab (Eriocheir sinensis). The pH values and digestive enzyme activities of the fermentation broth were measured. Five prebiotics (GOS, MOS, β-glucan, IMO, and XOS) exhibited significant effects, and they were subsequently selected for further analysis of short-chain fatty acid (SCFA) production and gut microbiota composition. After 12 h of in vitro fermentation, the MOS-supplemented group presented minimum pH and maximum trypsin and lipase activities. MOS outperformed other prebiotics, particularly butyric acid, in stimulating SCFA production. Compared with the other treatments, MOS significantly increased the growth of Clostridium and Lactococcus. Notably, MOS-driven butyrate accumulation was linked to the upregulation of anti-inflammatory pathways (e.g., NF-κB inhibition) and enhanced intestinal barrier integrity. Furthermore, MOS suppressed branched-chain fatty acids (BCFAs), potential markers of proteolytic dysbiosis, suggesting its role in mitigating gut inflammation. These findings highlight MOS as a potent immunomodulatory prebiotic for E. sinensis, providing a mechanistic basis for its application in antibiotic-free aquaculture strategies. |
| format | Article |
| id | doaj-art-e847bf03fdc14391bf9971a197367496 |
| institution | Kabale University |
| issn | 2352-5134 |
| language | English |
| publishDate | 2025-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj-art-e847bf03fdc14391bf9971a1973674962025-08-20T04:00:32ZengElsevierAquaculture Reports2352-51342025-09-014310295810.1016/j.aqrep.2025.102958Mannan-oligosaccharide modulates gut microbiota and enhances immune-metabolic functions in Chinese mitten crab (Eriocheir sinensis): Insights from an in vitro fermentation modelWei Li0Shubin Liu1Kaiqi Huang2Xiaoling Zhang3Xiaodan Wang4Jian G. Qin5Liqiao Chen6Erchao Li7School of Life Sciences, East China Normal University, Shanghai 200241, ChinaShanghai Fisheries Research Institute, Shanghai Fisheries Technical Extension Station, Shanghai 200433, China; Corresponding authors.School of Life Sciences, East China Normal University, Shanghai 200241, ChinaShanghai Ruijie River Crab Seed Co., Ltd., Shanghai 201716, ChinaSchool of Life Sciences, East China Normal University, Shanghai 200241, ChinaCollege of Science and Engineering, Flinders University, Adelaide, SA 5001, AustraliaSchool of Life Sciences, East China Normal University, Shanghai 200241, ChinaSchool of Life Sciences, East China Normal University, Shanghai 200241, China; Corresponding authors.Prebiotics are promising alternatives to antibiotics for enhancing disease resistance in aquaculture, yet their immunomodulatory mechanisms remain poorly understood.This study investigated the regulatory effects of prebiotics through in vitro experiments by directly comparing the effects of seven prebiotics, fructo-oligosaccharides (FOSs), galacto-oligosaccharides (GOSs), Mannan-oligosaccharides (MOSs), inulin, β-glucan, isomaltodextrin (IMO), and xylo-oligosaccharides (XOSs), on the gut microbiota and metabolites in the Chinese Mitten Crab (Eriocheir sinensis). The pH values and digestive enzyme activities of the fermentation broth were measured. Five prebiotics (GOS, MOS, β-glucan, IMO, and XOS) exhibited significant effects, and they were subsequently selected for further analysis of short-chain fatty acid (SCFA) production and gut microbiota composition. After 12 h of in vitro fermentation, the MOS-supplemented group presented minimum pH and maximum trypsin and lipase activities. MOS outperformed other prebiotics, particularly butyric acid, in stimulating SCFA production. Compared with the other treatments, MOS significantly increased the growth of Clostridium and Lactococcus. Notably, MOS-driven butyrate accumulation was linked to the upregulation of anti-inflammatory pathways (e.g., NF-κB inhibition) and enhanced intestinal barrier integrity. Furthermore, MOS suppressed branched-chain fatty acids (BCFAs), potential markers of proteolytic dysbiosis, suggesting its role in mitigating gut inflammation. These findings highlight MOS as a potent immunomodulatory prebiotic for E. sinensis, providing a mechanistic basis for its application in antibiotic-free aquaculture strategies.http://www.sciencedirect.com/science/article/pii/S2352513425003448PrebioticsIn vitro fermentationEriocheir sinensisImmune modulationGut microbiota |
| spellingShingle | Wei Li Shubin Liu Kaiqi Huang Xiaoling Zhang Xiaodan Wang Jian G. Qin Liqiao Chen Erchao Li Mannan-oligosaccharide modulates gut microbiota and enhances immune-metabolic functions in Chinese mitten crab (Eriocheir sinensis): Insights from an in vitro fermentation model Aquaculture Reports Prebiotics In vitro fermentation Eriocheir sinensis Immune modulation Gut microbiota |
| title | Mannan-oligosaccharide modulates gut microbiota and enhances immune-metabolic functions in Chinese mitten crab (Eriocheir sinensis): Insights from an in vitro fermentation model |
| title_full | Mannan-oligosaccharide modulates gut microbiota and enhances immune-metabolic functions in Chinese mitten crab (Eriocheir sinensis): Insights from an in vitro fermentation model |
| title_fullStr | Mannan-oligosaccharide modulates gut microbiota and enhances immune-metabolic functions in Chinese mitten crab (Eriocheir sinensis): Insights from an in vitro fermentation model |
| title_full_unstemmed | Mannan-oligosaccharide modulates gut microbiota and enhances immune-metabolic functions in Chinese mitten crab (Eriocheir sinensis): Insights from an in vitro fermentation model |
| title_short | Mannan-oligosaccharide modulates gut microbiota and enhances immune-metabolic functions in Chinese mitten crab (Eriocheir sinensis): Insights from an in vitro fermentation model |
| title_sort | mannan oligosaccharide modulates gut microbiota and enhances immune metabolic functions in chinese mitten crab eriocheir sinensis insights from an in vitro fermentation model |
| topic | Prebiotics In vitro fermentation Eriocheir sinensis Immune modulation Gut microbiota |
| url | http://www.sciencedirect.com/science/article/pii/S2352513425003448 |
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