Biotransformation of Indole to 3-Methylindole by Lysinibacillus xylanilyticus Strain MA
An indole-biotransforming strain MA was identified as Lysinibacillus xylanilyticus on the basis of the 16S rRNA gene sequencing. It transforms indole completely from the broth culture in the presence of an additional carbon source (i.e., sodium succinate). Gas-chromatography-mass spectrometry identi...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2015/425329 |
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| author | Pankaj Kumar Arora Kartik Dhar Rafael Alejandro Veloz García Ashutosh Sharma |
| author_facet | Pankaj Kumar Arora Kartik Dhar Rafael Alejandro Veloz García Ashutosh Sharma |
| author_sort | Pankaj Kumar Arora |
| collection | DOAJ |
| description | An indole-biotransforming strain MA was identified as Lysinibacillus xylanilyticus on the basis of the 16S rRNA gene sequencing. It transforms indole completely from the broth culture in the presence of an additional carbon source (i.e., sodium succinate). Gas-chromatography-mass spectrometry identified indole-3-acetamide, indole-3-acetic acid, and 3-methylindole as transformation products. Tryptophan-2-monooxygenase activity was detected in the crude extracts of indole-induced cells of strain MA, which confirms the formation of indole-3-acetamide from tryptophan in the degradation pathway of indole. On the basis of identified metabolites and enzyme assay, we have proposed a new transformation pathway for indole degradation. Indole was first transformed to indole-3-acetamide via tryptophan. Indole-3-acetamide was then transformed to indole-3-acetic acid that was decarboxylated to 3-methylindole. This is the first report of a 3-methylindole synthesis via the degradation pathway of indole. |
| format | Article |
| id | doaj-art-e846aa11b3e8454591b1141162aa1317 |
| institution | DOAJ |
| issn | 2090-9063 2090-9071 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Chemistry |
| spelling | doaj-art-e846aa11b3e8454591b1141162aa13172025-08-20T03:21:03ZengWileyJournal of Chemistry2090-90632090-90712015-01-01201510.1155/2015/425329425329Biotransformation of Indole to 3-Methylindole by Lysinibacillus xylanilyticus Strain MAPankaj Kumar Arora0Kartik Dhar1Rafael Alejandro Veloz García2Ashutosh Sharma3School of Biotechnology, Yeungnam University, Gyeongsan 712-749, Republic of KoreaDepartment of Microbiology, University of Chittagong, Chittagong 4331, BangladeshDivisión de Ciencias de la Salud, Departamento de Ingenieria Agroindustrial, Universidad de Guanajuato, Salvatierra, GTO, MexicoEscuela de Ingenieria en Alimentos, Biotecnologia y Agronomia, Instituto Tecnológico y de Estudios Superiores de Monterrey, Epigmenio Gonzalez 500, 76130 San Pablo, QRO, MexicoAn indole-biotransforming strain MA was identified as Lysinibacillus xylanilyticus on the basis of the 16S rRNA gene sequencing. It transforms indole completely from the broth culture in the presence of an additional carbon source (i.e., sodium succinate). Gas-chromatography-mass spectrometry identified indole-3-acetamide, indole-3-acetic acid, and 3-methylindole as transformation products. Tryptophan-2-monooxygenase activity was detected in the crude extracts of indole-induced cells of strain MA, which confirms the formation of indole-3-acetamide from tryptophan in the degradation pathway of indole. On the basis of identified metabolites and enzyme assay, we have proposed a new transformation pathway for indole degradation. Indole was first transformed to indole-3-acetamide via tryptophan. Indole-3-acetamide was then transformed to indole-3-acetic acid that was decarboxylated to 3-methylindole. This is the first report of a 3-methylindole synthesis via the degradation pathway of indole.http://dx.doi.org/10.1155/2015/425329 |
| spellingShingle | Pankaj Kumar Arora Kartik Dhar Rafael Alejandro Veloz García Ashutosh Sharma Biotransformation of Indole to 3-Methylindole by Lysinibacillus xylanilyticus Strain MA Journal of Chemistry |
| title | Biotransformation of Indole to 3-Methylindole by Lysinibacillus xylanilyticus Strain MA |
| title_full | Biotransformation of Indole to 3-Methylindole by Lysinibacillus xylanilyticus Strain MA |
| title_fullStr | Biotransformation of Indole to 3-Methylindole by Lysinibacillus xylanilyticus Strain MA |
| title_full_unstemmed | Biotransformation of Indole to 3-Methylindole by Lysinibacillus xylanilyticus Strain MA |
| title_short | Biotransformation of Indole to 3-Methylindole by Lysinibacillus xylanilyticus Strain MA |
| title_sort | biotransformation of indole to 3 methylindole by lysinibacillus xylanilyticus strain ma |
| url | http://dx.doi.org/10.1155/2015/425329 |
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