Macrophage-specific PHGDH protects against MAFLD by suppressing TAK1

Summary: Metabolic dysfunction-associated fatty liver disease (MAFLD) is a progressive disease with only one approved treatment currently available. Hepatic phosphoglycerate dehydrogenase (PHGDH), the rate-limiting enzyme of the serine biosynthesis pathway, regulates MAFLD development. However, the...

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Main Authors: Penghui Hu, Xiao Shan, Hongyuan Dong, Sujun Yu, Baochen Wang, Hui Xiong, Zemin Ji, Weijia Jing, Yan Cui, Zihan Li, Yanzhao Zhou, Zhe Wang, Jinrong Wang, Jiuzhou Tang, Ting Wang, Keliang Xie, Qiujing Yu
Format: Article
Language:English
Published: Elsevier 2025-03-01
Series:Cell Reports
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Online Access:http://www.sciencedirect.com/science/article/pii/S2211124725001974
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Summary:Summary: Metabolic dysfunction-associated fatty liver disease (MAFLD) is a progressive disease with only one approved treatment currently available. Hepatic phosphoglycerate dehydrogenase (PHGDH), the rate-limiting enzyme of the serine biosynthesis pathway, regulates MAFLD development. However, the role of macrophage PHGDH in MAFLD progression remains unclear. Here, we demonstrate that the lipotoxicity inducer palmitic acid (PA) significantly increases macrophage PHGDH expression and that PHGDH deficiency in macrophages promotes PA-induced inflammatory responses. Myeloid-specific PHGDH deficiency exacerbates MAFLD in mice. Mechanistically, tetrameric PHGDH binds to transforming growth factor-β-activated kinase 1 (TAK1) to inhibit its interaction with TAK1 binding protein 1 (TAB1), sequentially suppressing the activation of TAK1 and downstream NF-κB and MAPK signaling. Inhibition of TAK1 activation slows the development of metabolic dysfunction-associated steatohepatitis (MASH) caused by myeloid PHGDH knockout. Importantly, adeno-associated virus-mediated PHGDH overexpression in liver macrophages alleviates MAFLD in mice. Collectively, these results identify macrophage PHGDH as a promising therapeutic agent for MAFLD.
ISSN:2211-1247