Extended-spectrum and Metallo-beta lactamase enzymes mediated resistance in Pseudomonas aeruginosa in clinically isolated specimens
Pseudomonas aeruginosa is one of the leading opportunistic pathogens, frequently highlighted for the production of ESBL and MBL enzymes. This is believed to be the primary inhabitant of soil due to its adaptive nature and can survive in aquatic and even in toxic environment. The current study was a...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2021-04-01
|
| Series: | Kuwait Journal of Science |
| Subjects: | |
| Online Access: | https://journalskuwait.org/kjs/index.php/KJS/article/view/8495 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850231233837006848 |
|---|---|
| author | Farooq Ali Seema Kamal Qismat Shakeela Shehzad Ahmed |
| author_facet | Farooq Ali Seema Kamal Qismat Shakeela Shehzad Ahmed |
| author_sort | Farooq Ali |
| collection | DOAJ |
| description |
Pseudomonas aeruginosa is one of the leading opportunistic pathogens, frequently highlighted for the production of ESBL and MBL enzymes. This is believed to be the primary inhabitant of soil due to its adaptive nature and can survive in aquatic and even in toxic environment. The current study was aimed to screen ESBL and MBL producing and MDR strains of P. aeruginosa. Clinical specimens collected from patients and were screened for the presence of P. aeruginosa. After identification, all the isolates were tested for the sensitivity pattern following Kirby-Bauer disc diffusion method. The presence of ESBL and MBL enzymes were detected following DDST and IMP-EDTA detection tests respectively. Totally 1369 clinical specimens were collected, among which 126 (9.20%) specimens grown the colonies of P. aeruginosa on culture media. 54.76% (69/126) of the positive specimens confirmed in female population, whereas, 45.24% (57/126) in male population. High frequency rate, (n=43/126) was detected in age group ≥31 year, followed by 21-30 (n=35/126), and 11-20 (n=34/126) age group, the minimal frequency (n=14/126) was detected in age group 0-14. The sensitivity pattern showed that majority of the isolates were resistant, but class carbapenem, aminoglycosides and some other antibiotics i.e. sulbactam-Cefoperazone, tazobactam-piperacillin, tigecycline and Fosfomycin showed best results to P. aeruginosa. it is concluded that the emergence of resistance due to ESBL and MBL enzymes in P. aeruginosa is directly linked with public health concern because these strains are almost resistant to a wide range of antibiotics and only limited antibiotics are potent against these organisms.
|
| format | Article |
| id | doaj-art-e8408f76aef6436a86355fd802fdec29 |
| institution | OA Journals |
| issn | 2307-4108 2307-4116 |
| language | English |
| publishDate | 2021-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Kuwait Journal of Science |
| spelling | doaj-art-e8408f76aef6436a86355fd802fdec292025-08-20T02:03:36ZengElsevierKuwait Journal of Science2307-41082307-41162021-04-0148210.48129/kjs.v48i2.8495Extended-spectrum and Metallo-beta lactamase enzymes mediated resistance in Pseudomonas aeruginosa in clinically isolated specimensFarooq Ali0Seema Kamal1Qismat Shakeela2Shehzad Ahmed3Department of Microbiology, Hazara University Mansehra, 21300, KP, Pakistan.Department of Microbiology, Hazara University Mansehra, KP, Pakistan.Department of Microbiology, Hazara University, Mansehra, 21300, KP, PakistanDepartment of Microbiology, Hazara University, Mansehra, 21300, KP, Pakistan Pseudomonas aeruginosa is one of the leading opportunistic pathogens, frequently highlighted for the production of ESBL and MBL enzymes. This is believed to be the primary inhabitant of soil due to its adaptive nature and can survive in aquatic and even in toxic environment. The current study was aimed to screen ESBL and MBL producing and MDR strains of P. aeruginosa. Clinical specimens collected from patients and were screened for the presence of P. aeruginosa. After identification, all the isolates were tested for the sensitivity pattern following Kirby-Bauer disc diffusion method. The presence of ESBL and MBL enzymes were detected following DDST and IMP-EDTA detection tests respectively. Totally 1369 clinical specimens were collected, among which 126 (9.20%) specimens grown the colonies of P. aeruginosa on culture media. 54.76% (69/126) of the positive specimens confirmed in female population, whereas, 45.24% (57/126) in male population. High frequency rate, (n=43/126) was detected in age group ≥31 year, followed by 21-30 (n=35/126), and 11-20 (n=34/126) age group, the minimal frequency (n=14/126) was detected in age group 0-14. The sensitivity pattern showed that majority of the isolates were resistant, but class carbapenem, aminoglycosides and some other antibiotics i.e. sulbactam-Cefoperazone, tazobactam-piperacillin, tigecycline and Fosfomycin showed best results to P. aeruginosa. it is concluded that the emergence of resistance due to ESBL and MBL enzymes in P. aeruginosa is directly linked with public health concern because these strains are almost resistant to a wide range of antibiotics and only limited antibiotics are potent against these organisms. https://journalskuwait.org/kjs/index.php/KJS/article/view/8495wordsAntimicrobial resistanceDDSTESBLimipenem-EDTA testMBL |
| spellingShingle | Farooq Ali Seema Kamal Qismat Shakeela Shehzad Ahmed Extended-spectrum and Metallo-beta lactamase enzymes mediated resistance in Pseudomonas aeruginosa in clinically isolated specimens Kuwait Journal of Science words Antimicrobial resistance DDST ESBL imipenem-EDTA test MBL |
| title | Extended-spectrum and Metallo-beta lactamase enzymes mediated resistance in Pseudomonas aeruginosa in clinically isolated specimens |
| title_full | Extended-spectrum and Metallo-beta lactamase enzymes mediated resistance in Pseudomonas aeruginosa in clinically isolated specimens |
| title_fullStr | Extended-spectrum and Metallo-beta lactamase enzymes mediated resistance in Pseudomonas aeruginosa in clinically isolated specimens |
| title_full_unstemmed | Extended-spectrum and Metallo-beta lactamase enzymes mediated resistance in Pseudomonas aeruginosa in clinically isolated specimens |
| title_short | Extended-spectrum and Metallo-beta lactamase enzymes mediated resistance in Pseudomonas aeruginosa in clinically isolated specimens |
| title_sort | extended spectrum and metallo beta lactamase enzymes mediated resistance in pseudomonas aeruginosa in clinically isolated specimens |
| topic | words Antimicrobial resistance DDST ESBL imipenem-EDTA test MBL |
| url | https://journalskuwait.org/kjs/index.php/KJS/article/view/8495 |
| work_keys_str_mv | AT farooqali extendedspectrumandmetallobetalactamaseenzymesmediatedresistanceinpseudomonasaeruginosainclinicallyisolatedspecimens AT seemakamal extendedspectrumandmetallobetalactamaseenzymesmediatedresistanceinpseudomonasaeruginosainclinicallyisolatedspecimens AT qismatshakeela extendedspectrumandmetallobetalactamaseenzymesmediatedresistanceinpseudomonasaeruginosainclinicallyisolatedspecimens AT shehzadahmed extendedspectrumandmetallobetalactamaseenzymesmediatedresistanceinpseudomonasaeruginosainclinicallyisolatedspecimens |