DNA metabarcode analyses reveal similarities and differences in plant microbiomes of industrial hemp and medicinal Cannabis in China
Endophytic bacteria within plant tissues play crucial roles in plant health, stress tolerance, and contribute to the metabolite diversity of host plants. Cannabis sativa L. is an economically significant plant, with industrial hemp (IH) and medicinal Cannabis (MC) being the two main cultivars. Howev...
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Frontiers Media S.A.
2025-04-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1524703/full |
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| author | Jiayang Li Jiayang Li Hong Zhang Songhua Long Wenting Li Tuhong Wang Jian Yu Ying Zhou Shuo Zou Hongjian Zhu Jianping Xu Yi Cheng Yi Cheng |
| author_facet | Jiayang Li Jiayang Li Hong Zhang Songhua Long Wenting Li Tuhong Wang Jian Yu Ying Zhou Shuo Zou Hongjian Zhu Jianping Xu Yi Cheng Yi Cheng |
| author_sort | Jiayang Li |
| collection | DOAJ |
| description | Endophytic bacteria within plant tissues play crucial roles in plant health, stress tolerance, and contribute to the metabolite diversity of host plants. Cannabis sativa L. is an economically significant plant, with industrial hemp (IH) and medicinal Cannabis (MC) being the two main cultivars. However, the composition and functional traits of their endophytic bacterial communities in roots and leaves are not well understood. In this study, DNA metabarcode sequencing were employed to compare the bacterial communities between IH and MC. Significant differences were observed in the root and leaf niches. IH roots were enriched with stress-tolerant bacteria, while MC roots showed higher levels of biofilm-forming bacteria. In leaves, differences were even more pronounced, particularly in the abundance of Gram-negative bacteria, potential pathogens, stress-tolerant bacteria, and biofilm-forming bacteria. PICRUSt2 functional predictions revealed differences in nitrogen metabolism and secondary metabolite biosynthesis pathways in different cultivars and niches, while FAPROTAX analysis highlighted variations in carbon, nitrogen, and sulfur cycling functions. These findings underscore the distinct roles of bacterial communities in regulating plant health, stress responses, and metabolic processes in different niches and cultivars, providing insights for improving cultivation practices and plant resilience. |
| format | Article |
| id | doaj-art-e83e2e34fef4453abe9dc1a099a19f01 |
| institution | OA Journals |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj-art-e83e2e34fef4453abe9dc1a099a19f012025-08-20T02:26:59ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-04-011610.3389/fmicb.2025.15247031524703DNA metabarcode analyses reveal similarities and differences in plant microbiomes of industrial hemp and medicinal Cannabis in ChinaJiayang Li0Jiayang Li1Hong Zhang2Songhua Long3Wenting Li4Tuhong Wang5Jian Yu6Ying Zhou7Shuo Zou8Hongjian Zhu9Jianping Xu10Yi Cheng11Yi Cheng12Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Changsha, Hunan, ChinaInstitute of Bast Fiber Crops and Center of Southern Economic Crops, Chinese Academy of Agricultural Sciences, Changsha, Hunan, ChinaShenzhen Noposion Crop Science Co., Ltd., Shenzhen, Guangdong, ChinaInstitute of Bast Fiber Crops and Center of Southern Economic Crops, Chinese Academy of Agricultural Sciences, Changsha, Hunan, ChinaInstitute of Bast Fiber Crops and Center of Southern Economic Crops, Chinese Academy of Agricultural Sciences, Changsha, Hunan, ChinaInstitute of Bast Fiber Crops and Center of Southern Economic Crops, Chinese Academy of Agricultural Sciences, Changsha, Hunan, ChinaXishuangbanna Dai Autonomous Prefecture Tea Industry Development Service Center, Jinghong, Yunnan, ChinaInstitute of Agricultural Sciences of Xishuangbanna Prefecture of Yunnan Province, Jinghong, Yunnan, ChinaChangsha Agricultural and Rural Bureau, Changsha, Hunan, ChinaHunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Changsha, Hunan, ChinaDepartment of Biology, McMaster University, Hamilton, ON, CanadaInstitute of Bast Fiber Crops and Center of Southern Economic Crops, Chinese Academy of Agricultural Sciences, Changsha, Hunan, ChinaHunan Provincial Key Laboratory of the Traditional Chinese Medicine Agricultural Biogenomics, Changsha Medical University, Changsha, ChinaEndophytic bacteria within plant tissues play crucial roles in plant health, stress tolerance, and contribute to the metabolite diversity of host plants. Cannabis sativa L. is an economically significant plant, with industrial hemp (IH) and medicinal Cannabis (MC) being the two main cultivars. However, the composition and functional traits of their endophytic bacterial communities in roots and leaves are not well understood. In this study, DNA metabarcode sequencing were employed to compare the bacterial communities between IH and MC. Significant differences were observed in the root and leaf niches. IH roots were enriched with stress-tolerant bacteria, while MC roots showed higher levels of biofilm-forming bacteria. In leaves, differences were even more pronounced, particularly in the abundance of Gram-negative bacteria, potential pathogens, stress-tolerant bacteria, and biofilm-forming bacteria. PICRUSt2 functional predictions revealed differences in nitrogen metabolism and secondary metabolite biosynthesis pathways in different cultivars and niches, while FAPROTAX analysis highlighted variations in carbon, nitrogen, and sulfur cycling functions. These findings underscore the distinct roles of bacterial communities in regulating plant health, stress responses, and metabolic processes in different niches and cultivars, providing insights for improving cultivation practices and plant resilience.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1524703/fullbacterial diversitycommunity compositionCannabis sativa L.community differencesfunctional prediction |
| spellingShingle | Jiayang Li Jiayang Li Hong Zhang Songhua Long Wenting Li Tuhong Wang Jian Yu Ying Zhou Shuo Zou Hongjian Zhu Jianping Xu Yi Cheng Yi Cheng DNA metabarcode analyses reveal similarities and differences in plant microbiomes of industrial hemp and medicinal Cannabis in China Frontiers in Microbiology bacterial diversity community composition Cannabis sativa L. community differences functional prediction |
| title | DNA metabarcode analyses reveal similarities and differences in plant microbiomes of industrial hemp and medicinal Cannabis in China |
| title_full | DNA metabarcode analyses reveal similarities and differences in plant microbiomes of industrial hemp and medicinal Cannabis in China |
| title_fullStr | DNA metabarcode analyses reveal similarities and differences in plant microbiomes of industrial hemp and medicinal Cannabis in China |
| title_full_unstemmed | DNA metabarcode analyses reveal similarities and differences in plant microbiomes of industrial hemp and medicinal Cannabis in China |
| title_short | DNA metabarcode analyses reveal similarities and differences in plant microbiomes of industrial hemp and medicinal Cannabis in China |
| title_sort | dna metabarcode analyses reveal similarities and differences in plant microbiomes of industrial hemp and medicinal cannabis in china |
| topic | bacterial diversity community composition Cannabis sativa L. community differences functional prediction |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1524703/full |
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