High-precision immune-related plasma proteomics profiling predicts response to immunotherapy in patients with triple-negative breast cancer

Objective: While immunotherapy holds great potential for triple-negative breast cancer (TNBC), the lack of non-invasive biomarkers to identify beneficiaries limits the application. Methods: Paired baseline, on-treatment, and post-treatment plasma samples were collected from 195 TNBC patients receivi...

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Main Authors: Yuling Xiao, Hang Zhang, Yi Xiao, Ying Wang, Jing Zhang, Qi Hua, Pengchen Hu, Xinyan Lyu, Weihua Shou, Xin Hu, Zhiming Shao, Yizhou Jiang, Wei Huang, Jinxiu Shi
Format: Article
Language:English
Published: China Anti-Cancer Association 2025-07-01
Series:Cancer Biology & Medicine
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Online Access:https://www.cancerbiomed.org/content/22/7/854
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author Yuling Xiao
Hang Zhang
Yi Xiao
Ying Wang
Jing Zhang
Qi Hua
Pengchen Hu
Xinyan Lyu
Weihua Shou
Xin Hu
Zhiming Shao
Yizhou Jiang
Wei Huang
Jinxiu Shi
author_facet Yuling Xiao
Hang Zhang
Yi Xiao
Ying Wang
Jing Zhang
Qi Hua
Pengchen Hu
Xinyan Lyu
Weihua Shou
Xin Hu
Zhiming Shao
Yizhou Jiang
Wei Huang
Jinxiu Shi
author_sort Yuling Xiao
collection DOAJ
description Objective: While immunotherapy holds great potential for triple-negative breast cancer (TNBC), the lack of non-invasive biomarkers to identify beneficiaries limits the application. Methods: Paired baseline, on-treatment, and post-treatment plasma samples were collected from 195 TNBC patients receiving anti-PD-1 immunotherapy in this retrospective study conducted at the Fudan University Shanghai Cancer Center (FUSCC) for sequential high-precision proteomic profiling. Results: ARG1, NOS3, and CD28 were identified as plasma proteins significantly associated with the response to immunotherapy in neoadjuvant settings or in advanced stages of TNBC. Matched single-cell RNA sequencing data were incorporated to correlate peripheral plasma with the tumor microenvironment. Furthermore, the Plasma Immuno Prediction Score was developed to demonstrate significant predictive power for evaluating the efficacy and prognosis of patients undergoing neoadjuvant immunotherapy. Conclusions: The results underscore the importance of systemic immunity in the immunotherapy response and support the use of plasma protein profiles as a feasible tool for enhancing personalized management of immunotherapy in breast cancer.
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institution Kabale University
issn 2095-3941
language English
publishDate 2025-07-01
publisher China Anti-Cancer Association
record_format Article
series Cancer Biology & Medicine
spelling doaj-art-e83e11393def44efaf957112f8cc31c92025-08-20T03:35:12ZengChina Anti-Cancer AssociationCancer Biology & Medicine2095-39412025-07-0122785487010.20892/j.issn.2095-3941.2025.0038High-precision immune-related plasma proteomics profiling predicts response to immunotherapy in patients with triple-negative breast cancerYuling Xiao0Hang Zhang1Yi Xiao2Ying Wang3Jing Zhang4Qi Hua5Pengchen Hu6Xinyan Lyu7Weihua Shou8Xin Hu9Zhiming Shao10Yizhou Jiang11Wei Huang12Jinxiu Shi13Key Laboratory of Breast Cancer in Shanghai, Department of Breast Surgery, Fudan University Shanghai Cancer Center; Department of Oncology, Shanghai Medical College, Fudan University, Shanghai 200032, ChinaKey Laboratory of Breast Cancer in Shanghai, Department of Breast Surgery, Fudan University Shanghai Cancer Center; Department of Oncology, Shanghai Medical College, Fudan University, Shanghai 200032, ChinaKey Laboratory of Breast Cancer in Shanghai, Department of Breast Surgery, Fudan University Shanghai Cancer Center; Department of Oncology, Shanghai Medical College, Fudan University, Shanghai 200032, ChinaShanghai-MOST Key Laboratory of Health and Disease Genomics, Shanghai Institute for Biomedical and Pharmaceutical Technologies (SIBPT), Shanghai 200237, ChinaShanghai-MOST Key Laboratory of Health and Disease Genomics, Shanghai Institute for Biomedical and Pharmaceutical Technologies (SIBPT), Shanghai 200237, ChinaShanghai-MOST Key Laboratory of Health and Disease Genomics, Shanghai Institute for Biomedical and Pharmaceutical Technologies (SIBPT), Shanghai 200237, ChinaShanghai-MOST Key Laboratory of Health and Disease Genomics, Shanghai Institute for Biomedical and Pharmaceutical Technologies (SIBPT), Shanghai 200237, ChinaShanghai-MOST Key Laboratory of Health and Disease Genomics, Shanghai Institute for Biomedical and Pharmaceutical Technologies (SIBPT), Shanghai 200237, ChinaShanghai-MOST Key Laboratory of Health and Disease Genomics, Shanghai Institute for Biomedical and Pharmaceutical Technologies (SIBPT), Shanghai 200237, ChinaKey Laboratory of Breast Cancer in Shanghai, Department of Breast Surgery, Fudan University Shanghai Cancer Center; Department of Oncology, Shanghai Medical College, Fudan University, Shanghai 200032, ChinaKey Laboratory of Breast Cancer in Shanghai, Department of Breast Surgery, Fudan University Shanghai Cancer Center; Department of Oncology, Shanghai Medical College, Fudan University, Shanghai 200032, ChinaKey Laboratory of Breast Cancer in Shanghai, Department of Breast Surgery, Fudan University Shanghai Cancer Center; Department of Oncology, Shanghai Medical College, Fudan University, Shanghai 200032, ChinaShanghai-MOST Key Laboratory of Health and Disease Genomics, Shanghai Institute for Biomedical and Pharmaceutical Technologies (SIBPT), Shanghai 200237, ChinaShanghai-MOST Key Laboratory of Health and Disease Genomics, Shanghai Institute for Biomedical and Pharmaceutical Technologies (SIBPT), Shanghai 200237, ChinaObjective: While immunotherapy holds great potential for triple-negative breast cancer (TNBC), the lack of non-invasive biomarkers to identify beneficiaries limits the application. Methods: Paired baseline, on-treatment, and post-treatment plasma samples were collected from 195 TNBC patients receiving anti-PD-1 immunotherapy in this retrospective study conducted at the Fudan University Shanghai Cancer Center (FUSCC) for sequential high-precision proteomic profiling. Results: ARG1, NOS3, and CD28 were identified as plasma proteins significantly associated with the response to immunotherapy in neoadjuvant settings or in advanced stages of TNBC. Matched single-cell RNA sequencing data were incorporated to correlate peripheral plasma with the tumor microenvironment. Furthermore, the Plasma Immuno Prediction Score was developed to demonstrate significant predictive power for evaluating the efficacy and prognosis of patients undergoing neoadjuvant immunotherapy. Conclusions: The results underscore the importance of systemic immunity in the immunotherapy response and support the use of plasma protein profiles as a feasible tool for enhancing personalized management of immunotherapy in breast cancer.https://www.cancerbiomed.org/content/22/7/854triple-negative breast cancerimmunotherapynon-invasive biomarkersefficacy predictionplasma proteomic profiling
spellingShingle Yuling Xiao
Hang Zhang
Yi Xiao
Ying Wang
Jing Zhang
Qi Hua
Pengchen Hu
Xinyan Lyu
Weihua Shou
Xin Hu
Zhiming Shao
Yizhou Jiang
Wei Huang
Jinxiu Shi
High-precision immune-related plasma proteomics profiling predicts response to immunotherapy in patients with triple-negative breast cancer
Cancer Biology & Medicine
triple-negative breast cancer
immunotherapy
non-invasive biomarkers
efficacy prediction
plasma proteomic profiling
title High-precision immune-related plasma proteomics profiling predicts response to immunotherapy in patients with triple-negative breast cancer
title_full High-precision immune-related plasma proteomics profiling predicts response to immunotherapy in patients with triple-negative breast cancer
title_fullStr High-precision immune-related plasma proteomics profiling predicts response to immunotherapy in patients with triple-negative breast cancer
title_full_unstemmed High-precision immune-related plasma proteomics profiling predicts response to immunotherapy in patients with triple-negative breast cancer
title_short High-precision immune-related plasma proteomics profiling predicts response to immunotherapy in patients with triple-negative breast cancer
title_sort high precision immune related plasma proteomics profiling predicts response to immunotherapy in patients with triple negative breast cancer
topic triple-negative breast cancer
immunotherapy
non-invasive biomarkers
efficacy prediction
plasma proteomic profiling
url https://www.cancerbiomed.org/content/22/7/854
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