The role of adherent-to-suspension transition factors in clear cell renal cell carcinoma progression: a comprehensive analysis
Abstract Recent studies have identified a biological process called adherent-to-suspension transition (AST) as a key factor in promoting metastasis. The involvement of AST in the progression of clear cell renal cell carcinoma (ccRCC) remains largely unexplored. We comprehensively investigated the ov...
Saved in:
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-04-01
|
| Series: | Scientific Reports |
| Subjects: | |
| Online Access: | https://doi.org/10.1038/s41598-025-96807-9 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849699290814873600 |
|---|---|
| author | Chenglong Xu Haotian Wei Yue Wang Xiaocheng Ma Qi Li Yingjie Bi Zifei Li Zhaochen Li Shimiao Zhu Jing Tian Changyi Quan Shiyong Qi |
| author_facet | Chenglong Xu Haotian Wei Yue Wang Xiaocheng Ma Qi Li Yingjie Bi Zifei Li Zhaochen Li Shimiao Zhu Jing Tian Changyi Quan Shiyong Qi |
| author_sort | Chenglong Xu |
| collection | DOAJ |
| description | Abstract Recent studies have identified a biological process called adherent-to-suspension transition (AST) as a key factor in promoting metastasis. The involvement of AST in the progression of clear cell renal cell carcinoma (ccRCC) remains largely unexplored. We comprehensively investigated the overall landscape of all 20 AST factors at the pan-cancer level. The risk stratification method and the prognostic model based on AST factors were established with consensus clustering analysis and LASSO regression algorithm. The potential molecular mechanism of AST was further investigated by multi-omics analysis and cell experiments. We found that AST factors play distinct roles in different cancer types. Consequently, we utilized AST factors to develop a risk stratification method and a prognostic model, which can effectively guide the treatment of ccRCC patients. We proposed that the promotion of AST is facilitated by SPIB through the SAA1-AKT pathway, enhancing the likelihood of ccRCC metastasis, and conducted rigorous analyses using multi-omics data and cellular experiments. Our study presents a novel risk stratification method and prognostic model for ccRCC. And we identified the SPIB-SAA1-AKT pathway as one of the potential mechanisms by which AST factors promote ccRCC metastasis. |
| format | Article |
| id | doaj-art-e837b45674cf47938e4c549a67d6c52b |
| institution | DOAJ |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-e837b45674cf47938e4c549a67d6c52b2025-08-20T03:18:38ZengNature PortfolioScientific Reports2045-23222025-04-0115111510.1038/s41598-025-96807-9The role of adherent-to-suspension transition factors in clear cell renal cell carcinoma progression: a comprehensive analysisChenglong Xu0Haotian Wei1Yue Wang2Xiaocheng Ma3Qi Li4Yingjie Bi5Zifei Li6Zhaochen Li7Shimiao Zhu8Jing Tian9Changyi Quan10Shiyong Qi11Department of Urology, The Second Hospital of Tianjin Medical UniversityDepartment of Urology, The Second Hospital of Tianjin Medical UniversityDepartment of Urology, The Second Hospital of Tianjin Medical UniversityDepartment of Urology, The Second Hospital of Tianjin Medical UniversityDepartment of Urology, The Second Hospital of Tianjin Medical UniversityDepartment of Urology, The Second Hospital of Tianjin Medical UniversityDepartment of Urology, The Second Hospital of Tianjin Medical UniversityDepartment of Urology, The Second Hospital of Tianjin Medical UniversityDepartment of Urology, The Second Hospital of Tianjin Medical UniversityDepartment of Urology, The Second Hospital of Tianjin Medical UniversityDepartment of Urology, The Second Hospital of Tianjin Medical UniversityDepartment of Urology, The Second Hospital of Tianjin Medical UniversityAbstract Recent studies have identified a biological process called adherent-to-suspension transition (AST) as a key factor in promoting metastasis. The involvement of AST in the progression of clear cell renal cell carcinoma (ccRCC) remains largely unexplored. We comprehensively investigated the overall landscape of all 20 AST factors at the pan-cancer level. The risk stratification method and the prognostic model based on AST factors were established with consensus clustering analysis and LASSO regression algorithm. The potential molecular mechanism of AST was further investigated by multi-omics analysis and cell experiments. We found that AST factors play distinct roles in different cancer types. Consequently, we utilized AST factors to develop a risk stratification method and a prognostic model, which can effectively guide the treatment of ccRCC patients. We proposed that the promotion of AST is facilitated by SPIB through the SAA1-AKT pathway, enhancing the likelihood of ccRCC metastasis, and conducted rigorous analyses using multi-omics data and cellular experiments. Our study presents a novel risk stratification method and prognostic model for ccRCC. And we identified the SPIB-SAA1-AKT pathway as one of the potential mechanisms by which AST factors promote ccRCC metastasis.https://doi.org/10.1038/s41598-025-96807-9Adherent-to-suspension transitionClear cell renal cell carcinomaPrognostic modelMulti-omics studyPan-cancer analysis |
| spellingShingle | Chenglong Xu Haotian Wei Yue Wang Xiaocheng Ma Qi Li Yingjie Bi Zifei Li Zhaochen Li Shimiao Zhu Jing Tian Changyi Quan Shiyong Qi The role of adherent-to-suspension transition factors in clear cell renal cell carcinoma progression: a comprehensive analysis Scientific Reports Adherent-to-suspension transition Clear cell renal cell carcinoma Prognostic model Multi-omics study Pan-cancer analysis |
| title | The role of adherent-to-suspension transition factors in clear cell renal cell carcinoma progression: a comprehensive analysis |
| title_full | The role of adherent-to-suspension transition factors in clear cell renal cell carcinoma progression: a comprehensive analysis |
| title_fullStr | The role of adherent-to-suspension transition factors in clear cell renal cell carcinoma progression: a comprehensive analysis |
| title_full_unstemmed | The role of adherent-to-suspension transition factors in clear cell renal cell carcinoma progression: a comprehensive analysis |
| title_short | The role of adherent-to-suspension transition factors in clear cell renal cell carcinoma progression: a comprehensive analysis |
| title_sort | role of adherent to suspension transition factors in clear cell renal cell carcinoma progression a comprehensive analysis |
| topic | Adherent-to-suspension transition Clear cell renal cell carcinoma Prognostic model Multi-omics study Pan-cancer analysis |
| url | https://doi.org/10.1038/s41598-025-96807-9 |
| work_keys_str_mv | AT chenglongxu theroleofadherenttosuspensiontransitionfactorsinclearcellrenalcellcarcinomaprogressionacomprehensiveanalysis AT haotianwei theroleofadherenttosuspensiontransitionfactorsinclearcellrenalcellcarcinomaprogressionacomprehensiveanalysis AT yuewang theroleofadherenttosuspensiontransitionfactorsinclearcellrenalcellcarcinomaprogressionacomprehensiveanalysis AT xiaochengma theroleofadherenttosuspensiontransitionfactorsinclearcellrenalcellcarcinomaprogressionacomprehensiveanalysis AT qili theroleofadherenttosuspensiontransitionfactorsinclearcellrenalcellcarcinomaprogressionacomprehensiveanalysis AT yingjiebi theroleofadherenttosuspensiontransitionfactorsinclearcellrenalcellcarcinomaprogressionacomprehensiveanalysis AT zifeili theroleofadherenttosuspensiontransitionfactorsinclearcellrenalcellcarcinomaprogressionacomprehensiveanalysis AT zhaochenli theroleofadherenttosuspensiontransitionfactorsinclearcellrenalcellcarcinomaprogressionacomprehensiveanalysis AT shimiaozhu theroleofadherenttosuspensiontransitionfactorsinclearcellrenalcellcarcinomaprogressionacomprehensiveanalysis AT jingtian theroleofadherenttosuspensiontransitionfactorsinclearcellrenalcellcarcinomaprogressionacomprehensiveanalysis AT changyiquan theroleofadherenttosuspensiontransitionfactorsinclearcellrenalcellcarcinomaprogressionacomprehensiveanalysis AT shiyongqi theroleofadherenttosuspensiontransitionfactorsinclearcellrenalcellcarcinomaprogressionacomprehensiveanalysis AT chenglongxu roleofadherenttosuspensiontransitionfactorsinclearcellrenalcellcarcinomaprogressionacomprehensiveanalysis AT haotianwei roleofadherenttosuspensiontransitionfactorsinclearcellrenalcellcarcinomaprogressionacomprehensiveanalysis AT yuewang roleofadherenttosuspensiontransitionfactorsinclearcellrenalcellcarcinomaprogressionacomprehensiveanalysis AT xiaochengma roleofadherenttosuspensiontransitionfactorsinclearcellrenalcellcarcinomaprogressionacomprehensiveanalysis AT qili roleofadherenttosuspensiontransitionfactorsinclearcellrenalcellcarcinomaprogressionacomprehensiveanalysis AT yingjiebi roleofadherenttosuspensiontransitionfactorsinclearcellrenalcellcarcinomaprogressionacomprehensiveanalysis AT zifeili roleofadherenttosuspensiontransitionfactorsinclearcellrenalcellcarcinomaprogressionacomprehensiveanalysis AT zhaochenli roleofadherenttosuspensiontransitionfactorsinclearcellrenalcellcarcinomaprogressionacomprehensiveanalysis AT shimiaozhu roleofadherenttosuspensiontransitionfactorsinclearcellrenalcellcarcinomaprogressionacomprehensiveanalysis AT jingtian roleofadherenttosuspensiontransitionfactorsinclearcellrenalcellcarcinomaprogressionacomprehensiveanalysis AT changyiquan roleofadherenttosuspensiontransitionfactorsinclearcellrenalcellcarcinomaprogressionacomprehensiveanalysis AT shiyongqi roleofadherenttosuspensiontransitionfactorsinclearcellrenalcellcarcinomaprogressionacomprehensiveanalysis |