Flavonoids from sour jujube leaves: Ultrasound-assisted extraction, UPLC-QQQ-MS/MS quantification, and ameliorative effect on DSS-induced ulcerative colitis in mice
To valorize sour jujube (Ziziphus acidojujuba) leaves, this work focused on the extraction, quantification, and bioactivity assessment of flavonoids. First, ultrasound-assisted extraction (UAE) was employed to extract total flavonoids from sour jujube leaves (SJL-TF), with the procedure optimized th...
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Elsevier
2025-03-01
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| Series: | Ultrasonics Sonochemistry |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1350417725000586 |
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| author | Hongke Wei Xiaomin Wang Jiaqi Wang Shijie Ren Luis A.J. Mur Dong Lu Duo Cao |
| author_facet | Hongke Wei Xiaomin Wang Jiaqi Wang Shijie Ren Luis A.J. Mur Dong Lu Duo Cao |
| author_sort | Hongke Wei |
| collection | DOAJ |
| description | To valorize sour jujube (Ziziphus acidojujuba) leaves, this work focused on the extraction, quantification, and bioactivity assessment of flavonoids. First, ultrasound-assisted extraction (UAE) was employed to extract total flavonoids from sour jujube leaves (SJL-TF), with the procedure optimized through single-factor design and response surface methodology (RSM). SJL-TF yield reached 48.47 ± 0.36 mg/g under optimal circumstances, which included 61 % ethanol as extraction medium, an ultrasound power of 300 W, an extraction time of 33 min, and a liquid–solid ratio of 16 mL/g, showing higher extraction efficiency in comparison to the traditional Soxhlet extraction method. Scanning electron microscopy (SEM) analysis indicated that ultrasound treatment severely damaged the structural integrity of sour jujube leaves, which was more conducive to improving the SJL-TF yield. Then, polyamide resin chromatography was used to purify the crude SJL-TF extracts, increasing the SJL-TF purity by 3.2-fold to 74.58 ± 0.63 %. By developing and validating a UPLC-QQQ-MS/MS method, ten main flavonoids in SJL-TF extracts, including catechin, rutin, isoquercetin, narirutin, nicotiflorin, quercitrin, phlorizin, luteolin, quercetin, and apigenin, were successfully detected concurrently for quality control purposes. Furthermore, the purified SJL-TF showed a strong ameliorative effect on dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) in mice, as evidenced by significant mitigation of colonic inflammation and pathological damage. Thus, this work will contribute to improving the application of sour jujube leaves, especially in the pharmaceutical sector. |
| format | Article |
| id | doaj-art-e836c178a6e94f1f9a87b4376fee5ed1 |
| institution | DOAJ |
| issn | 1350-4177 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
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| series | Ultrasonics Sonochemistry |
| spelling | doaj-art-e836c178a6e94f1f9a87b4376fee5ed12025-08-20T03:11:55ZengElsevierUltrasonics Sonochemistry1350-41772025-03-0111410727910.1016/j.ultsonch.2025.107279Flavonoids from sour jujube leaves: Ultrasound-assisted extraction, UPLC-QQQ-MS/MS quantification, and ameliorative effect on DSS-induced ulcerative colitis in miceHongke Wei0Xiaomin Wang1Jiaqi Wang2Shijie Ren3Luis A.J. Mur4Dong Lu5Duo Cao6Shaanxi Key Laboratory of Research and Utilization of Resource Plants on the Loess Plateau, College of Life Sciences, Yan’an University, Yan’an 716000 Shaanxi, ChinaShaanxi Key Laboratory of Research and Utilization of Resource Plants on the Loess Plateau, College of Life Sciences, Yan’an University, Yan’an 716000 Shaanxi, ChinaShaanxi Key Laboratory of Research and Utilization of Resource Plants on the Loess Plateau, College of Life Sciences, Yan’an University, Yan’an 716000 Shaanxi, ChinaShaanxi Key Laboratory of Research and Utilization of Resource Plants on the Loess Plateau, College of Life Sciences, Yan’an University, Yan’an 716000 Shaanxi, ChinaDepartment of Life Sciences, Aberystwyth University, Ceredigion SY23 3DA, UKShanghai Frontiers Science Center of TCM Chemical Biology, Institute of Interdisciplinary Integrative Medicine Research, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China; Corresponding authors.Shaanxi Key Laboratory of Research and Utilization of Resource Plants on the Loess Plateau, College of Life Sciences, Yan’an University, Yan’an 716000 Shaanxi, China; Corresponding authors.To valorize sour jujube (Ziziphus acidojujuba) leaves, this work focused on the extraction, quantification, and bioactivity assessment of flavonoids. First, ultrasound-assisted extraction (UAE) was employed to extract total flavonoids from sour jujube leaves (SJL-TF), with the procedure optimized through single-factor design and response surface methodology (RSM). SJL-TF yield reached 48.47 ± 0.36 mg/g under optimal circumstances, which included 61 % ethanol as extraction medium, an ultrasound power of 300 W, an extraction time of 33 min, and a liquid–solid ratio of 16 mL/g, showing higher extraction efficiency in comparison to the traditional Soxhlet extraction method. Scanning electron microscopy (SEM) analysis indicated that ultrasound treatment severely damaged the structural integrity of sour jujube leaves, which was more conducive to improving the SJL-TF yield. Then, polyamide resin chromatography was used to purify the crude SJL-TF extracts, increasing the SJL-TF purity by 3.2-fold to 74.58 ± 0.63 %. By developing and validating a UPLC-QQQ-MS/MS method, ten main flavonoids in SJL-TF extracts, including catechin, rutin, isoquercetin, narirutin, nicotiflorin, quercitrin, phlorizin, luteolin, quercetin, and apigenin, were successfully detected concurrently for quality control purposes. Furthermore, the purified SJL-TF showed a strong ameliorative effect on dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) in mice, as evidenced by significant mitigation of colonic inflammation and pathological damage. Thus, this work will contribute to improving the application of sour jujube leaves, especially in the pharmaceutical sector.http://www.sciencedirect.com/science/article/pii/S1350417725000586Sour jujube leavesFlavonoidsUltrasound-assisted extractionUPLC-QQQ-MS/MSUlcerative colitis |
| spellingShingle | Hongke Wei Xiaomin Wang Jiaqi Wang Shijie Ren Luis A.J. Mur Dong Lu Duo Cao Flavonoids from sour jujube leaves: Ultrasound-assisted extraction, UPLC-QQQ-MS/MS quantification, and ameliorative effect on DSS-induced ulcerative colitis in mice Ultrasonics Sonochemistry Sour jujube leaves Flavonoids Ultrasound-assisted extraction UPLC-QQQ-MS/MS Ulcerative colitis |
| title | Flavonoids from sour jujube leaves: Ultrasound-assisted extraction, UPLC-QQQ-MS/MS quantification, and ameliorative effect on DSS-induced ulcerative colitis in mice |
| title_full | Flavonoids from sour jujube leaves: Ultrasound-assisted extraction, UPLC-QQQ-MS/MS quantification, and ameliorative effect on DSS-induced ulcerative colitis in mice |
| title_fullStr | Flavonoids from sour jujube leaves: Ultrasound-assisted extraction, UPLC-QQQ-MS/MS quantification, and ameliorative effect on DSS-induced ulcerative colitis in mice |
| title_full_unstemmed | Flavonoids from sour jujube leaves: Ultrasound-assisted extraction, UPLC-QQQ-MS/MS quantification, and ameliorative effect on DSS-induced ulcerative colitis in mice |
| title_short | Flavonoids from sour jujube leaves: Ultrasound-assisted extraction, UPLC-QQQ-MS/MS quantification, and ameliorative effect on DSS-induced ulcerative colitis in mice |
| title_sort | flavonoids from sour jujube leaves ultrasound assisted extraction uplc qqq ms ms quantification and ameliorative effect on dss induced ulcerative colitis in mice |
| topic | Sour jujube leaves Flavonoids Ultrasound-assisted extraction UPLC-QQQ-MS/MS Ulcerative colitis |
| url | http://www.sciencedirect.com/science/article/pii/S1350417725000586 |
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