Genome assembly and annotation of Babesia rossi, a protozoan parasite for canine babesiosis
Abstract Background Apicomplexan parasite, Babesia rossi, is an Ixodid tick-transmitted pathogen that causes the most severe form of canine babesiosis disease. Compared to other Babesia pathogens of dogs, B. rossi exhibits unique pathophysiology, virulence, and a responsiveness to drugs that differs...
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2025-07-01
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| Online Access: | https://doi.org/10.1186/s12864-025-11495-z |
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| author | Neelam Redekar Xu Wang Luis Neves Steven Brooks Justin Lack Andrew Leisewitz Hans Ackerman |
| author_facet | Neelam Redekar Xu Wang Luis Neves Steven Brooks Justin Lack Andrew Leisewitz Hans Ackerman |
| author_sort | Neelam Redekar |
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| description | Abstract Background Apicomplexan parasite, Babesia rossi, is an Ixodid tick-transmitted pathogen that causes the most severe form of canine babesiosis disease. Compared to other Babesia pathogens of dogs, B. rossi exhibits unique pathophysiology, virulence, and a responsiveness to drugs that differs from the small Babesia parasites. Results Here we report the first near-complete chromosome-level assembly of Babesia rossi strain PMB – isolated from a sick dog from Pietermaritzburg, South Africa. Assembly with long-read HiFi data yielded 21.06 Mbp genome size, spanning across five gene-dense chromosome-level scaffolds, a single apicoplast scaffold, and a remaining 54 unplaced low gene density scaffolds with 1.32 Mb N50 and 96.6% BUSCO Apicomplexan completeness. The genome annotation identified a total of 3,098 protein-coding genes, 71 tRNA, and 16 rRNA genes. The mitochondrial genome (6.4 Kbp) was also identified. Genome assemblies of two additional field isolates of B. rossi were also reported. Comparative genomic analyses revealed four syntenic genomic inversions and multiple polymorphisms across three B. rossi isolates, although SNP and indel density was higher within the gene desserts of the genomes. Despite these differences, three B. rossi isolates’ genome assemblies showed 99% conserved orthologous gene sets. About 76% of protein-coding genes of Babesia rossi isolate PMB were shared with four other Babesia species. Conclusion This report provides valuable genomic information that is crucial to comprehend B. rossi evolution, virulence, and potential drug targets for canine babesiosis. |
| format | Article |
| id | doaj-art-e8322d65ee0d45ad8b28b1aa1ed3a065 |
| institution | DOAJ |
| issn | 1471-2164 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | BMC |
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| series | BMC Genomics |
| spelling | doaj-art-e8322d65ee0d45ad8b28b1aa1ed3a0652025-08-20T03:03:27ZengBMCBMC Genomics1471-21642025-07-0126111310.1186/s12864-025-11495-zGenome assembly and annotation of Babesia rossi, a protozoan parasite for canine babesiosisNeelam Redekar0Xu Wang1Luis Neves2Steven Brooks3Justin Lack4Andrew Leisewitz5Hans Ackerman6Integrated Data Sciences Section, Research Technologies Branch, National Institute of Allergy and Infectious Diseases, National Institutes of HealthDepartment of Pathobiology, College of Veterinary Medicine, Auburn UniversityVeterinary Tropical Diseases, Faculty of Veterinary Science, University of PretoriaLaboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Disease, National Institutes of HealthIntegrated Data Sciences Section, Research Technologies Branch, National Institute of Allergy and Infectious Diseases, National Institutes of HealthDepartment of Clinical Sciences, College of Veterinary Medicine, Auburn UniversityLaboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Disease, National Institutes of HealthAbstract Background Apicomplexan parasite, Babesia rossi, is an Ixodid tick-transmitted pathogen that causes the most severe form of canine babesiosis disease. Compared to other Babesia pathogens of dogs, B. rossi exhibits unique pathophysiology, virulence, and a responsiveness to drugs that differs from the small Babesia parasites. Results Here we report the first near-complete chromosome-level assembly of Babesia rossi strain PMB – isolated from a sick dog from Pietermaritzburg, South Africa. Assembly with long-read HiFi data yielded 21.06 Mbp genome size, spanning across five gene-dense chromosome-level scaffolds, a single apicoplast scaffold, and a remaining 54 unplaced low gene density scaffolds with 1.32 Mb N50 and 96.6% BUSCO Apicomplexan completeness. The genome annotation identified a total of 3,098 protein-coding genes, 71 tRNA, and 16 rRNA genes. The mitochondrial genome (6.4 Kbp) was also identified. Genome assemblies of two additional field isolates of B. rossi were also reported. Comparative genomic analyses revealed four syntenic genomic inversions and multiple polymorphisms across three B. rossi isolates, although SNP and indel density was higher within the gene desserts of the genomes. Despite these differences, three B. rossi isolates’ genome assemblies showed 99% conserved orthologous gene sets. About 76% of protein-coding genes of Babesia rossi isolate PMB were shared with four other Babesia species. Conclusion This report provides valuable genomic information that is crucial to comprehend B. rossi evolution, virulence, and potential drug targets for canine babesiosis.https://doi.org/10.1186/s12864-025-11495-zBabesiaGenomeBabesia rossiCanineProtozoanApicomplexa |
| spellingShingle | Neelam Redekar Xu Wang Luis Neves Steven Brooks Justin Lack Andrew Leisewitz Hans Ackerman Genome assembly and annotation of Babesia rossi, a protozoan parasite for canine babesiosis BMC Genomics Babesia Genome Babesia rossi Canine Protozoan Apicomplexa |
| title | Genome assembly and annotation of Babesia rossi, a protozoan parasite for canine babesiosis |
| title_full | Genome assembly and annotation of Babesia rossi, a protozoan parasite for canine babesiosis |
| title_fullStr | Genome assembly and annotation of Babesia rossi, a protozoan parasite for canine babesiosis |
| title_full_unstemmed | Genome assembly and annotation of Babesia rossi, a protozoan parasite for canine babesiosis |
| title_short | Genome assembly and annotation of Babesia rossi, a protozoan parasite for canine babesiosis |
| title_sort | genome assembly and annotation of babesia rossi a protozoan parasite for canine babesiosis |
| topic | Babesia Genome Babesia rossi Canine Protozoan Apicomplexa |
| url | https://doi.org/10.1186/s12864-025-11495-z |
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